Number 53 May 2003
In February we flew to Washington DC so that Gretchen
could identify ascidians at the Smithsonian’s Edgewater MD site only to
be marooned in a hotel by snow for several days. We finally got there and
the ascidians got identified although it meant doing some (the remaining
1071!) at home in Seattle. In March we drove to southern California
where we presented a paper on the southern hemisphere Corella eumyota
that we found in France last summer (see AN#52 Work in Progress).
On the way back we visited Bill Smith at UC Santa Barbara to see his very
impressive facilities for rearing mutant Cionas. Soon after
returning from the California trip we flew to Prince Edward Island, Canada,
to participate in a workshop relating to the overgrowth of mussel lines
by millions of invasive Styela clava. Since this part of Canada
is mostly soft bottom, the only firm substrates are pilings, docks, and
the mussels and apparatus used in their culture. Since S. clava
is cultured for food in Korea, we hope that perhaps North American markets
can be developed for S. clava. Ciona intestinalis is
also a big problem particularly on oyster cages. Kevin Heasman from
New Zealand gave a talk on his extensive experience with invasive ascidians
and mussel culture in South Africa and New Zealand. Sea ice 2 feet
thick was a new experience for us. The S. clava survive just
fine all winter below the ice. In May we presented a hands-on display
of the Sea Squirts of the San Juans for the open house at the Friday Harbor
Laboratories which was the formal kick-off of next year’s centennial celebration.
We also gave a short talk on the history of the laboratories. Also
in May we joined the Smithsonian invasions group to survey Tampa Bay, Florida,
for invasive ascidians, and gave an ascidian identification workshop at
Bodega marine lab in northern California. Mid June to late July we
will work at the Friday Harbor Labs, August we’ll participate in a nonindigenous
species survey along the NE U.S. coast from Maine to New York, and in September
we join the Smithsonian group again for a week in the Ketchikan area.
There are 116 New Publications citations at the end of this issue—definitely there is something of interest to everyone working on ascidians. If you have not yet sent us your new publications, we would greatly appreciate receiving a hard copy if available, or pdf.
*Ascidian News is not part of the scientific literature and should not be cited as such.
NEWS AND VIEWS
1. Patrick Lemaire (firstname.lastname@example.org),
Marseille, France is organizing an International Urochordate Meeting
for Oct 11-14, 2003, at Carry-le Rouet, France, near Marseille. This
meeting will focus on evolutionary, developmental and functional genomics
aspects of work carried out in our favorite animals. An advisory
committee (Mike Levine, Hiroki Nishida, Yasushi Okamura, Christian Sardet,
Nori Satoh, and Billie Swalla) will select speakers on the basis of the
submittted abstracts. For registration and abstract forms see: http://www.lgpd.univ-mrs.fr/more_info/lemaire/Front_Page.htm
There you can find the preliminary programme, registration form, abstract submission form and the meeeting venue website. The deadline for the abstract submission is May 31, and the abstracts can only be accepted after your registration (see information on registration form and abstract submission form). For more information write to: email@example.com
The following sessions are planned:
Oct 12 Session 1: Urochordate evolution
Session 2: Early embryonic patterning
Oct 13 Session 3: Cell biological approaches
Session 4: Neural development
Oct 14 Session 5: Of genomes and large scale approaches
Session 6: Round tables on community-oriented tools
2. From Anna Di Gregorio (firstname.lastname@example.org):
After completing her postdoctoral work in the laboratory of Michael Levine
at UC Berkeley, Anna Di Gregorio has recently moved to the East Coast and
has set up her ascidian lab in the Department of Cell and Developmental
Biology, at the Weill Medical College of Cornell University in Manhattan
(NY). The main focus of the new lab is to study the evolutionary origins
of notochord and other chordate tissues using as a main model system Ciona
intestinalis, and in the future, other marine invertebrates, representative
of sister phyla.
Collaborations, visits and suggestions are welcome!
3. The new ascidian website developed by Arjan Gittenberger (email@example.com), National Museum of Natural History, Naturalis, Leiden The Netherlands, is expanding rapidly, with many more species added in the past 6 months. Each entry includes a color photo, location and some information. At present there are a total of 235 photographs of 146 ascidian species/morphs. Please visit the site at www.ascidians.com. Arjan writes: If anyone has any suggestions (e.g. for better identifications of the species), please let me know.” Contributions are welcomed.
4. From David Keys (firstname.lastname@example.org)
and Dario Boffelli (email@example.com)
Ciona intestinalis samples needed for polymorphism study.
We are writing to asking your help putting together a global collection of Ciona intestinalis genomic DNA. As most of you know, the polymorphism rate in Ciona has turned out to be quite high. (OK, amazingly high.) The two haplotypes from the single California Ciona intestinalis used to produce the genomic sequence here at the Joint Genome Institute had a 1.2% polymorphism rate. We (Dario Boffelli at the Lawrence Berkeley National Labs and David Keys at the JGI) are setting up a small project at LBNL to characterize just how much polymorphism is out there. We’ve got several goals. The one we suspect will be most important to the general community will be a simple assessment of how close the sequence hosted at the JGI (http://www.jgi.doe.gov/ciona/index.html) is to the sequences of animals pulled from various locations around the world. In addition we are hypothesizing that it may be possible to locate functional regions, such as coding or perhaps even cis-reg domains, by looking for regions of low polymorphism. This is similar to an approach that Dario has been doing using human, ape and monkey sequences (Boffelli et al. (2003) Phylogenetic shadowing of primate sequences to find functional regions of the human genome. Science. 299:1391-4). Currently we are not sure whether there will be enough polymorphism for this. However, any study to assess this can easily be set up to produce data for the general polymorphism assessment.
Our initial plan is to generate sequence for a few genomic intervals containing enhancer, promoter and exon regions from multiple Ciona intestinalis individuals from as many world wide locations as possible. We would like your help in gathering the tissue samples for this. If possible, we would like to do the DNA preps here to keep the protocol standardized. For any scientist who sends material, we will make sequence data from those animals available to them immediately so they can assess how their experimental population relates to the JGI reference genome.
If you can supply material for this study, please contact us for collecting and shipping info (obviously, we'll pay for shipping). We’d also appreciate any contacts you could send our way for commercial or marine lab collectors who might not otherwise see this message.
5. The element vanadium was named “in honor of Vanadis, the Norse goddess of beauty” (quoted from Uncle Tungsten, Memories of a Chemical Boyhood by Oliver Sacks 2001). Vanadium salts occur in many beautiful colors, depending on what it is combined with and which valency is present. Even a very tiny amount, just a few atoms, of this transition element, can be enough to produce brilliant colors. “Because it had four strikingly different valencies or oxidation states, and it was easy to transform these into one another, vanadium was an ideal element to experiment with.” Sacks as a boy would start with a test tube of pentavalent ammonium vanadate in solution, add zinc amalgam, and the solution would change from yellow to royal blue, the color of tetravalent vanadium. If allowed to react further until the vanadium changed to trivalent, the solution turned green. “If one waited still longer, the green would disappear and be replaced by a beautiful lilac, the color of divalent vanadium. The reverse experiment was even more beautiful, especially if one layered potassium permanganate, a deep purple layer, over the delicate lilac; this would be oxidized over a period of hours and form separate layers, one above the other, of lilac divalent vanadium on the bottom, then green trivalent, then blue tetravalent, then yellow pentavalent vanadium.”
WORK IN PROGRESS
1. Elisabetta Tosti, Cell Biology lab, Stazione
Zoologica, Villa Comunale, 80121 Napoli, Italy firstname.lastname@example.org
: Bioactive aldehydes from diatoms block the fertilization current in
ascidian oocytes by E. Tosti, G. Romano, I. Buttino, A. Cuomo, A. Ianora,
A. Miralto, in press, Molecular Reproduction and Development.
Abstract: We studied the effects of bioactive aldehydes from diatoms, unicellular algae at the base of the marine food web, on fertilization and early developmental processes of the ascidian Ciona intestinalis. Using electrophysiological techniques, we showed that the fertilization current which is generated in oocytes upon interaction with the spermatozoon was inhibited when oocytes were pre-incubated in 2-trans-4-trans-decadienal (DD). This event was accompanied by the inhibition of the voltage-gated calcium current activity of the plasma membrane, whereas DD did not inhibit the subsequent contraction of the cortex. DD did not act as a channel inhibitor since it did not affect the steady state conductance of the plasma membrane or gap junctional communication within blastomeres of the embryo. On the other hand, DD did affect actin reorganisation differently from that of other actin blockers. Possibly this effect on actin reorganisation was responsible for the subsequent teratogenic action on larval development. A reversibility of DD effect indicates that it may specifically target certain fertilization mechanisms. Diatom aldehydes have been shown to interfere with reproductive mechanisms in copepods, polychaetes and echinoderms. Our data provide evidence that such compounds may also adversely affect ascidian fertilization mechanisms.
In this paper we show that diatom reactive aldehydes such as DD may have a dual effect on reproductive processes, influencing primary fertilization events such as gating of fertilization channels and secondary processes such as actin reorganisation which is responsible for the segregation of cell lines. These findings add to a growing body of evidence on the antiproliferative effects of diatom-derived aldehydes. Our results also report, for the first time, on the action of a fertilization channel blocker in marine invertebrates.
2. George Mackie and C.L. Singla,
Univ. of Victoria, Victoria, BC, Canada. email@example.com:
The cupular strand of Corella inflata (Ascidiacea): a sense organ with GnRH-immunoreactive primary sensory neurons. In preparation.
The cupular strand of Corella resembles the cupular organs of Ciona but unlike the latter, which are isolated, dome-shaped structures located in the inner mantle epithelium, each containing 15-20 sensory cells, the cupular strand is a single long structure containing some 1500 sensory cells and located not in the mantle but in the branchial sac, where it runs along the dorsal midline of the dorsal fold. Preparations have been examined by optical and electron microscopy, and also by immunofluorescence microscopy using anti-tubulin and anti-gonadotropin-releasing hormone (GnRH) antisera. The sensory cells and their axons were immunoreactive with anti-tunicate I GnRH antiserum, but not with antisera against catfish and chicken GnRH. All three GnRH antisera however labelled the dorsal strand plexus, a nerve net that runs in the dorsal blood sinus with extensions around the brain and in the vicinity of the gonads and gonoducts. It is suggested that the sensory neurons contain a different form of GnRH from the one(s) contained in the dorsal strand plexus and that it may function as a neuromodulator affecting ciliary beating in the branchial sac. The dorsal strand plexus on the other hand is probably the source of a form (or forms) of GnRH that regulate reproductive processes. It was possible to follow the sensory axons from the macula of the cupular strand to points where they disappeared into branches of the visceral nerve, which enters a nerve root at the back of the brain.
The function of the cupular strand has not been investigated physiologically but it seems likely that it is a hydrodynamic sensor registering changes in water flow velocity through the atrial cavity. In discussing these findings we compare the cupular strand of Corella with putative hydrodynamic sensors described in other ascidians, and we consider the distribution and possible functions of GnRH cells in Corella in the context of existing information on the locations of such cells and their significance in ascidians generally. [This paper continues similar research on a related species reported in a recently published article: Mackie GO & Singla CL 2003. The capsular organ of Chelyosoma productum (Ascidiacea: Corellidae):a new tunicate hydrodynamic sense organ. Brain Behav. Evol. 61:45-58.]
3. From Patrick Frank, Dept. of Chemistry, Stanford Univ., Stanford, CA 94305-5080 (Frank@ssrl.slac.stanford.edu): We recently completed a study on vanadium in blood cells from Ascidia ceratodes as found in Bodega Bay, California, and included a comparison with analogous results for A. ceratodes in Monterey Bay, CA (P. Frank, R.M.K. Carlson, E.J. Carlson and K.O. Hodgson 2003 "The vanadium environment in blood cells of Ascidia ceratodes is divergent at all organismal levels: an XAS and EPR spectroscopic study" J. Inorg. Biochem. 94: 59-71). Essentially, both the blood cell vanadium content and the intracellular acidity vary virtually from cell-to-cell within a given animal, from animal-to-animal, and, on average, between the populations of Monterey Bay and Bodega Bay, CA. The signet-ring blood cell intravacuolar acidity in the Bodega A. ceratodes is as low as pH 0, and on average is noticably more acidic than the Monterey population. This pH difference produces observable differences in the distributions of dissolved vanadium complexes. We've also noted that Bodega individuals average much larger than the Monterey. Frozen blood cells also vary from uniformly canary-yellow (Bodega) to yellow-green (Monterey). One wonders whether there may be an incipient speciation event in progress there.
4. Ryo Koyanagi
and Thomas G. Honegger (firstname.lastname@example.org)
, Zool. Inst., Univ. of Zürich, CH-8057 Zürich, Switzerland.
cloning and sequence analysis of an ascidian egg b-N-acetylhexosaminidase
with potential role in fertilization. In press, Develop. Growth
Beta-N-acetylhexosaminidase, which is found almost ubiquitously in sperm of invertebrates and vertebrates, supposedly mediates a carbohydrate-based transient sperm-egg coat binding. In ascidians and mammals beta-hexosaminidase released at fertilization from eggs has been proposed to modify sperm-receptor glycoproteins of the egg envelope, thus setting up a block to polyspermy. Previously, we showed that in potential sperm-receptor glycoproteins of the ascidian Phallusia mammillata N-acetylglucosamine is the prevailing glycoside residue and that the egg harbors three active molecular forms of b-hexosaminidase. In the present study, we have isolated and characterized P. mammillata beta-hexosaminidase cDNA from an ovarian cDNA library. The deduced amino acid sequence showed high similarities with other known b-hexosaminidases, on the other hand P. mammillata beta-hexosaminidase has an unique potential N-glycosylation site. A phylogenetic analysis suggests that the P. mammillata beta-hexosaminidase developed independently after having branched off from the common ancestor gene of the chordate enzyme before two isoforms of the mammalian enzyme appeared. In situ hybridization revealed stage-specific expression of b-hexosaminidase mRNA during oogenesis in the oocyte and the accessory test and follicle cells. This suggests that the three egg beta-hexosaminidase forms are specific for the oocyte, the test and follicle cells respectively.
5. Gretchen Lambert: New records of ascidians
from the NE Pacific: a new species of Trididemnum, range extension
and redescription of Aplidiopsis pannosum (Ritter, 1899) including
its larva, and several nonindigenous species. In press, Zoosystema
Abstract: A new species of aplousobranch ascidian, Trididemnum alexi, is described from the San Juan Archipelago of Washington state, USA. Colonies are smooth dark reddish brown; the largest is 8 cm in maximum width and up to 2 cm thick in some regions because of the large and complex hypozooidal cloacal canals. A thick (200-400 µm) superficial bladder cell layer is present. The spicules are 20-30 µm in diameter, irregularly stellate with short pointed rays (8-10 in optical equatorial plane) and scattered thinly through the tunic but absent from the bladder cell layer. Zooids have 3 rows of stigmata, with 12-13 stigmata per side in the first two rows in most zooids (occasionally 11 or 14) and usually 1-2 fewer in the third row. The tubular atrial siphon opens dorsal to the middle row of stigmata. The single testis is covered by a sperm duct with 8-9 coils. Larvae form in the basal portion of the colony, are 0.8-1 mm in trunk length with 3 adhesive papillae and usually 7 pairs of lateral ampullae with curved tips in the fully formed tadpoles. A redescription of Aplidiopsis pannosum (Ritter, 1899) includes the morphological analysis of several larval stages. This species, though widespread in the north Pacific, was not previously known to occur south of Alaska on the west coast of North America. Range extensions are also included for several nonindigenous ascidians: Ciona savignyi, Botrylloides violaceus, Styela clava and Molgula manhattensis.
6. Paolo Burighel,
L. Manni, G.
Zaniolo, N.J. Lane and colleagues (email@example.com),
Univ. of Padua, Italy.
We have demonstrated the presence of a new, possibly mechanoreceptor organ, "the coronal organ" in the oral siphons of botryllids (Botryllus schlosseri and Botrylloides leachi) (in press in J. Comparative Neurology vol. 461). This organ is composed of a line of sensory cells, accompanied by supporting cells that runs along the margin of the velum and the tentacles of the siphon. The sensory cells are "hair cells" resembling those of the vertebrate lateral line, or, in general, the acoustico-lateralis system, because they bear a single cilium, located centrally or eccentrically to a hair bundle of numerous stereovilli. In contrast to other sensory cells of ascidians, the coronal hair cells are secondary sensory cells, since they lack axonal processes directed towards the cerebral ganglion. Moreover, at their base, they form synapses with nerve fibres, most of which exhibit acetylcholinesterase activity. The absence of axonal extensions was confirmed by experiments with lipophilic dyes. Different kinds of synapses were recognised: usually, each hair cell forms a few afferent synapses with dendrites of neurons located in the ganglion; efferent synapses, both axo-somatic (between an axon coming from the ganglion and the hair cell) and axo-dendritic (between an axon coming from the ganglion and an afferent fibre) were occasionally found. In the paper we compare the secondary sensory cells of ascidians with the sensory cells and placodes of vertebrates and propose that the coronal organ is homologous to the vertebrate acoustico-lateralis system.
7. Carlo Brena (firstname.lastname@example.org),
Univ. of Padua, Italy.
During my PhD thesis I studied with light and electron microscopy (applying to it also histochemistry and immunohistochemistry) the alimentary tract of several species of appendicularian belonging to the three families of these tunicates. The results (The alimentary system of Appendicularians, simplifications, specializations, adaptation [in Italian]), show that the three families, especially from the point of view of the gut, are strikingly different from one another, with a strong separation, in particular, between Oikopleurids on one side and Fritillarids and Kowalevskids on the other side. From both the general morphology and the cytological differentiation, there are many more differences between these two groups than among all the other tunicates. In particular, they show ultrastructural specializations, so far apparently unique, e.g. some apical junctions between cells in Kowalevskia. The three families, in particular fritillarids and kowalevskids, show the body with strong simplification which might account for their extremely short life cycles and high biomass and dispersal in all oceans. In particular, fritillarids have some gut cells extremely specialized, probably involved in osmotic regulation. The results should arouse interest on these so far poorly known animals, from the ultrastructural, ecological/physiological and evolutionary points of view. As for this last point, a picture and some references on the exceptionality of these tunicates has been introduced in the newly released book The Development of Animal Form: Ontogeny Morphology and Evolution, by Alessandro Minelli, Cambridge Press.
Most of my thesis material has been or will soon be published; in addition to the articles already published on Oikopleura dioica digestive system (TEM: Burighel et al. 2001) and histochemistry (Cima et al. 2002) [see past issues of AN for complete citations], three articles are in press:
Brena, C., F. Cima & P. Burighel 2003. The highly specialised gut of Fritillariidae (Appendicularia, Tunicata). Marine Biology, in press.
Brena, C., F. Cima & P. Burighel 2003. The exceptional “blind” gut of Appendicularia sicula (Appendicularia, Tunicata). Zoologischer Anzeiger in press.
Brena, C., F. Cima & P. Burighel 2003. The alimentary tract of Kowalevskiidae (Appendicularia, Tunicata) and evolutionary implications. Journal of Morphology in press.
1. Aplousobranch ascidians (Tunicata: Ascidiacea)
from southern Africa. Shirley Parker-Nance, Dept. of Zool.,
Univ. of Port Elizabeth, S. Africa. Ph.D. thesis, awarded April 2003.
Advisor: Prof. T. Wooldridge Univ. of Port Elizabeth and Prof. M. Davies-Coleman,
Rhodes University. email@example.com,
The ascidian (subphylum Tunicata: class Ascidiacea) fauna along the southern African coast constitutes an important component of the sessile benthic reef fauna. Little is known of the species composition, biodiversity and distribution of ascidians on southern African intertidal and subtidal reefs. Past research on the ascidian fauna of South Africa was sporadic and limited, with only about one hundred and seventy species recorded during the last one hundred and twenty years. This is the first taxonomic study undertaken by a South African resident scientist. The study focused on six genera in four families (Euherdmaniidae, Polyclinidae, Pseudodistomidae and Didemnidae) belonging to the class Ascidiacea and suborder Aplousobranchia. Five species new to science, two Polyclinum, two Pseudodistoma and one Polysyncraton species are described. New additional information on the distribution of five species previously known to science, including one recorded for the first time along the South African coast, is presented. A literature review and comparison of the taxonomic important characteristics is made of all species known globally for the six genera. These six genera comprise 12 Euherdmania, 15 Aplidiopsis, 40 Polyclinum, 31 Pseudodistoma, 6 Atriolum and 68 Polysyncraton species.
2. Phylogeny of the Order Aplousobranchia (Tunicata,
Ascidiacea). Tatiane Regina Moreno, Dept. of Zoology, Universidade
Federal do Paraná, Brazil. (firstname.lastname@example.org)
Ph.D. Thesis, March 2003. Advisors Dr. Rosana Moreira da Rocha & Dr.
Walter A. P. Böeger.
The phylogeny of Aplousobranchia Lahille (Tunicata, Ascidiacea) sensu Kott (1969, 1990, 1992) was for the first time reconstructed using morphological characters. The relationships between genera and families were analyzed employing the principles of phylogenetic systematics (Hennig 1966; Wiley 1981) with PAUP. Monophyly of the Aplousobranchia and of the families from this order were tested with ascidians from 14 different families. A matrix with 40 taxa and 59 characters was analyzed using a heuristic search strategy: random stepwise addition (n=1000 replicates). Pyura Molina, 1782 and Molgula Forbes & Hanley, 1848 (Stolidobranchia) and Ascidia Linnaeus, 1767 and Perophora Wiegmann, 1835 (Phlebobranchia) were defined as outgroups. The analysis resulted in 154 equally most parsimonious trees (length 173, CI=0.5549, RI=0.8046). Characters correlated with replication process and the formation of colony systems, and branchial wall characters were more important in the phylogenetic reconstruction of Aplousobranchia than other characters traditionally used in Ascidiacea taxonomy, such as body division and position of the heart, gonads and epicardium. New characters included here for the first time such as body wall muscles, muscles associated with transversal blood vessels and arrangement of the larval papillae also have phylogenetic importance. The monophyly of Aplousobranchia sensu Lahille, including only Polycitoridae, Polyclinidae, and Didemnidae ascidians is supported. However, Aplousobranchia sensu Kott (1969, 1990, 1992) is not monophyletic because Perophora from outgroup was included in the cladogram in the ingroup. Several Aplousobranchia families are monophyletic. We proposed a classification based on the phylogenetic analysis, in which Aplousobranchia now has 17 families (two new), and some indeterminant taxa.
Molecular Biology Society of Japan 25th annual
meeting, December 11-14, 2002. Yokohama, Japan.
Michibata, H., Ueki, T. and Yamaguchi, N. Molecular physiological analysis of the mechanisms of vanadium accumulation in ascidians. [no abstract].
Adams, B. A., Tello, J. A., Erchegyi, J., Warby, C., Hong, D. J., Akinsanya, K. O., Mackie, G. O., Vale, W., Rivier, J. E. and Sherwood, N. M. 2003. Six novel gonadotropin-releasing hormones are encoded as triplets on each of two genes in the protochordate, Ciona intestinalis. Endocrinology 144: 1907-1919.
Aiello, A., Fattorusso, E., Luciano, P., Menna, M., Esposito, G., Iuvone, T. and Pala, D. 2003. Conicaquinones A and B, two novel cytotoxic terpene quinones from the Mediterranean ascidian Aplidium conicum. Europ. J. Org. Chem. March 2003: 898-900.
Aiello, A., Fattorusso, E., Mangoni, A. and Menna, M. 2003. Three new 2,3-dihydroxy fatty acid glycosphingolipids from the Mediterranean tunicate Microcosmus sulcatus. Europ. J. Org. Chem. Feb. 2003: 734-739.
Aizenberg, J., Weiner, S. and Addadi, L. 2003. Coexistence of amorphous and crystalline calcium carbonate in skeletal tissues. Conn. Tiss. Res. 44 Suppl. 1: 20-22.
Artman, G. D. and Weinreb, S. M. 2003. An approach to the total synthesis of the marine ascidian metabolite perophoramidine via a halogen-selective tandem Heck/carbonylation strategy. Org. Lett. 5: 1523-1526.
Azumi, K., Kuribayashi, F., Kanegasaki, S. and Yokosawa, H. 2002. Zymosan induces production of superoxide anions by hemocytes of the solitary ascidian Halocynthia roretzi. Comp. Biochem. Physiol. C 133: 567-574.
Boorman, C. J. and Shimeld, S. M. 2002. The evolution of left-right asymmetry in chordates. BioEssays 24: 1004-1011.
Burton, A. 2002. Sea squirt assaults sarcomas. Lancet Oncol. 3: 648.
Canestro, C., Bassham, S. and Postlethwait, J. H. 2003. Seeing chordate evolution through the Ciona genome sequence. Genome Biol. 4: 208.
Canestro, C., Godoy, L., Gonzalez-Duarte, R. and Albalat, R. 2003. Comparative expression analysis of Adh3 during arthropod, urochordate, cephalochordate, and vertebrate development challenges its predicted housekeeping role. Evol. & Dev. 5: 157-62.
Catlow, K., Deakin, J. A., Delehedde, M., Fernig, D. G., Gallagher, J. T., Pavao, M. S. G. and Lyon, M. 2003. Hepatocyte growth factor/scatter factor and its interaction with heparan sulphate and dermatan sulphate. Biochem. Soc. Trans. 31: 352-353.
Chiba, S., Awazu, S., Itoh, M., Chin-Bow, S. T., Satoh, N., Satou, Y. and Hastings, K. E. 2003. A genomewide survey of developmentally relevant genes in Ciona intestinalis IX. Genes for muscle structural proteins. Dev Genes Evol 10: 10.
Coles, S. L. and Eldredge, G. S. 2002. Nonindigenous species introductions on coral reefs: a need for information. Pac. Sci. 56: 191-209.
Cooper, E. L. and Parrinello, N. 2001. Immunodefense in tunicates: cells and molecules. In: Sawada, H., Yokosawa, H. and Lambert, C. C. (ed.), The Biology of Ascidians. Tokyo, Springer-Verlag, pp. 383-394.
Cruz-Monserrate, Z., Vervoort, H. C., Bai, R., Newman, D. J., Howell, S. B., Los, G., Mullaney, J. T., Williams, M. D., Pettit, G. R., Fenical, W. and Hamel, E. 2003. Diazonamide a and a synthetic structural analog: disruptive effects on mitosis and cellular microtubules and analysis of their interactions with tubulin. Mol. Pharmacol. 63: 1273-1280.
Dalfo, D., Albalat, R., Molotkov, A., Duester, G. and Gonzales-Duarte, R. 2002. Retinoic acid synthesis in the prevertebrate amphioxus involves retinol oxidation. Dev. Genes Evol. 212: 388-393.
D'Ambrosio, P., Fanelli, A., Pischetola, M. and Spagnuolo, A. 2003. Ci-GATAa, a GATA-class gene from the ascidian Ciona intestinalis: Isolation and developmental expression. Dev. Dyn. 226: 145-148.
Davis, R. A., Sandoval, I. T., Concepcion, G. P., Rocha, R. M. and Ireland, C. A. 2003. Lissoclinotoxins E and F, novel cytotoxic alkaloids from a Philippine didemnid ascidian. Tetrahedron 59: 2855-2859.
De Tomaso, A. W. and Weissman, I. L. 2003. Construction and characterization of large-insert genomic libraries (BAC and fosmid) from the ascidian Botryllus schlosseri and initial physical mapping of a histocompatibility locus. Mar. Biotechnol. 5: 103-115.
Dehal, P., Satou, Y., Campbell, R. K., Chapman, J., Degnan, B., De Tomaso, A., Davidson, B., Di Gregorio, A. and al., e. 2002. The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins. Science 298: 2157-2167.
Delfourne, E. and Bastide, J. 2003. Marine pyridoacridine alkaloids and synthetic analogues as antitumor agents. Med. Res. Rev. 23: 234-252.
Deschet, K., Nakatani, Y. and Smith, W. C. 2003. Generation of Ci-Brachyury-GFP stable transgenic lines in the ascidian Ciona savignyi. Genesis 35: 248-259.
Dumollard, R., Hammar, K., Porterfield, M., Smith, P. J., Cibert, C., Rouviere, C. and Sardet, C. 2003. Mitochondrial respiration and Ca2+ waves are linked during fertilization and meiosis completion. Development 130: 683-692.
Elphick, M. R., Satou, Y. and Satoh, N. 2003. The invertebrate ancestry of endocannabinoid signalling: an orthologue of vertebrate cannabinoid receptors in the urochordate Ciona intestinalis. Gene 302: 95-101.
Endo, Y., Nonaka, M., Saiga, H., Kakinuma, Y., Matsushita, A., Takahashi, M., Matsushita, M. and Fujita, T. 2003. Origin of mannose-binding lectin-associated serine protease (MASP)-1 and MASP-3 involved in the lectin complement pathway traced back to the invertebrate, amphioxus. J. Immunol. 170: 4701-4707.
Frank, P., Carlson, R. M. K., Carlson, E. J. and Hodgson, K. O. 2003. The vanadium environment in blood cells of Ascidia ceratodes is divergent at all organismal levels: an XAS and EPR spectroscopic study. J. Inorg. Chem. 94: 59-71.
Frank, P., Carlson, R. M. K., Carlson, E. J. and Hodgson, K. O. 2003. Medium-dependence of vanadium K-edge X-ray absorption spectra with application to blood cells from phlebobranch tunicates. Coord. Chem. Rev. 237: 31-39.
Fujita, M., Nakao, Y., Matsunaga, S., Nishikawa, T. and Fusetani, N. 2002. Sodium 1-(12-hydroxy)octadecanyl sulfate, an MMP2 inhibitor, isolated from a tunicate of the family Polyclinidae. J. Nat. Prod. 65: 1936-8.
Gajate, C., An, F. and Mollinedo, F. 2003. Rapid and selective apoptosis in human leukemic cells induced by aplidine through a fas/CD95-and mitochondrial-mediated mechanism. Clin. Cancer Res. 9: 1535-1545.
Garrido, L., Zubia, E., Ortega, M. J. and Salva, J. 2003. Haouamines A and B: a new class of alkaloids from the ascidian Aplidium haouarianum. J. Org. Chem. 68: 293-299.
Gorbman, A., Whiteley, A. and Kavanaugh, S. 2003. Pheromonal stimulation of spawning release of gametes by gonadotropin releasing hormone in the chiton, Mopalia sp. Gen. Comp. Endocrinol. 131: 62-65.
Gostling, N. J. and Shimeld, S. M. 2003. Protochordate Zic genes define primitive somite compartments and highlight molecular changes underlying neural crest evolution. Evol. & Dev. 5: 136-144.
Green, P. L., Nair, S. V. and Raftos, D. A. 2003. Secretion of a collectin-like protein in tunicates is enhanced during inflammatory responses. Dev. Comp. Immunol. 27: 3-9.
Hirose, E. 2003. Colonial allorecognition, hemolytic rejection,
and viviparity in botryllid ascidians. Zool. Sci. 20: 387-394.
Holland, P. W. 2002. Ciona. Curr. Biol. 12: R609.
Hudson, C., Darras, S., Caillol, D., Yasuo, H. and Lemaire, P. 2003. A conserved role for the MEK signalling pathway in neural tissue specification and posteriorisation in the invertebrate chordate, the ascidian Ciona intestinalis. 130: 147-159.
Imai, K. S., Satoh, N. and Satou, Y. 2002. Region specific gene expressions in the central nervous system of the ascidian embryo. Gene Expr. Patterns 2: 319-321.
Irie, T., Kajiwara, S. and Seki, T. 2003. Storage of retinal in the eggs of the ascidian, Halocynthia roretzi. Comp. Biochem. Physiol. B 134: 221-230.
Ishii, T. and Hirose, E. 2003. Fate of tunic phagocytes in the colonial ascidian Aplidium yamazii. Mem. Fac. Educ. Human Studies Akita Univ. 58: 37-41.
Issa, H. H., Tanaka, J., Rachmat, R. and Higa, T. 2003. Floresolides, new metacyclophane hydroquinone lactones from an ascidian, Aplidium sp. Tet. Lett. 44: 1243-1245.
Iwasa, T., Mishima, S., Watari, A., Ohkuma, M., Azuma, T., Kanehara, K. and Tsuda, M. 2003. A novel G protein alpha subunit in embryo of the ascidian, Halocynthia roretzi. Zool. Sci. 20: 141-151.
Kanamori, K. 2003. Structures and properties of multinuclear vanadium(III) complexes: seeking a clue to understand the role of vanadium(III) in ascidians. Coord. Chem. Rev. 237: 147-161.
Kawahara, G., Terakado, K., Tanaka, S. and Kikuyama, S. 2003. Occurrence of prohormone convertase-like substances in the neural complex cells of the ascidian Halocynthia roretzi. Gen. Comp. Endocrinol. 131: 32-37.
Kawano, M., Falandysz, J. and Wakimoto, T. 2003. Instrumental neutron activation analysis of extractable organohalogens (EOX) in Antarctic marine organisms. J. Radioanalyt. & Nuclear Chem. 255: 235-237.
Kawashima, T., Tokuoka, M., Awazu, S., Satoh, N. and Satou, Y. 2003. A genomewide survey of developmentally relevant genes in Ciona intestinalis VIII. Genes for PI3K signaling and cell cycle. Dev. Genes Evol. 13: 13.
Khalturin, K., Becker, M., Rinkevich, B. and Bosch, T. C. 2003. Urochordates and the origin of natural killer cells: identification of a CD94/NKR-P1-related receptor in blood cells of Botryllus. Proc Natl Acad Sci 100: 622-627.
Komiya, T., Fusetani, N., Matsunaga, S., Kubo, A., Kaye, F. J., Kelley, M. J., Tamura, K., Yoshida, M., Fukuoka, M. and Nakagawa, K. 2003. Ritterazine B, a new cytotoxic natural compound, induces apoptosis in cancer cells. Cancer Chemotherapy & Pharmacol. 51: 202-208.
Kondoh, M., Kasai, T., Shimada, M., Kashiwayanagi, M. and Yokosawa, H. 2003. cDNA cloning and characterization of an osmotically sensitive TRP channel from ascidian eggs. Comp. Biochem. Physiol. B 134: 417-423.
Lambert, G. 2003. Marine biodiversity of Guam: the Ascidiacea. Micronesica 35-36: 588-597.
Lemaire, P., Bertrand, V. and Hudson, C. 2002. Early steps in the formation of neural tissue in ascidian embryos. Dev. Biol. 252: 151-69.
Mackie, G. O. and Singla, C. L. 2003. The capsular organ of Chelyosoma productum (Ascidiacea: Corellidae): a new tunicate hydrodynamic sense organ. Brain Behav. & Evol. 61: 45-58.
Manzanares, I., Cuevas, C., Garcia-Nieto, R., Marco, E. and Gago, F. 2001. Advances in the chemistry and pharmacology of ecteinascidins, a promising new class of anti-cancer agents. Curr Med Chem Anti-Canc Agents 1: 257-76.
Marchenkov, A. and Boxshall, G. A. 2003. A new notodelphyid copepod, Paranotodelphys illgi n. sp. (Copepoda: Cyclopoida), parasitic in the ascidian Corynascidia herdmani Ritter in the North Pacific. Syst. Parasitol. 54: 43-52.
Marchini, S., Chiorino, G., Faircloth, G. T. and D'-Incalci, M. 2002. Changes in gene expression profile induced by the anticancer agent aplidine in molt-4 leukemic cell lines. J. Biol. Regulators & Homeostatic Agents 16: 241-248.
Marshall, D. J., Pechenik, J. A. and Keough, M. J. 2003. Larval activity levels and delayed metamorphosis affect post-larval performance in the colonial ascidian Diplosoma listerianum. Mar. Ecol. Prog. Ser. 246: 153–162.
Matsumoto, S. S., Biggs, J., Copp, B. R., Holden, J. A. and Barrows, L. R. 2003. Mechanism of ascididemin-induced cytotoxicity. Chem. Res. Toxicol. 16: 113-122.
McHenry, M. J., Azizi, E. and Strother, J. A. 2003. The hydrodynamics of locomotion at intermediate Reynolds numbers: undulatory swimming in ascidian larvae (Botrylloides sp.). J. Exp. Biol. 206: 327-343.
McKeever, B. and Pattenden, G. 2003. Total synthesis of the cytotoxic cyclopeptide mollamide, isolated from the sea squirt Didemnum molle. Tetrahedron 59: 2701-2712.
Michael, J. P. 2002. Indolizidine and quinolizidine alkaloids. Nat. Prod. Rep. 19: 719-741.
Michibata, H., Yamaguchi, N., Uyama, T. and Ueki, T. 2003. Molecular biological approaches to the accumulation and reduction of vanadium by ascidians. Coord. Chem. Rev. 237: 41-51.
Miya, T. and Nishida, H. 2003. Expression pattern and transcriptional control of SoxB1 in embryos of the ascidian Halocynthia roretzi. Zool. Sci. 20: 59-67.
Mochizuki, Y., Satou, Y. and Satoh, N. 2003. Large-scale characterization of genes specific to the larval nervous system in the ascidian Ciona intestinalis. Genesis 36: 62-71.
Nagatomo, K., Ishibashi, T., Satou, Y., Satoh, N. and Fujiwara, S. 2003. Retinoic acid affects gene expression and morphogenesis without upregulating the retinoic acid receptor in the ascidian Ciona intestinalis. Mech. Dev. 120: 363-72.
Nakajo, K., Katsuyama, Y., Ono, F., Ohtsuka, Y. and Okamura, Y. 2003. Primary structure, functional characterization and developmental expression of the ascidian Kv4-class potassium channel. Neurosci. Res. 45: 59-70.
Nakamura, Y., Makabe, K. W. and Nishida, H. 2003. Localization and expression pattern of type I postplasmic mRNAs in embryos of the ascidian Halocynthia roretzi. Gene Expr Patterns 3: 71-75.
Nakashima, Y., Kusakabe, T., Kusakabe, R., Terakita, A., Shichida, Y. and Tsuda, M. 2003. Origin of the vertebrate visual cycle: Genes encoding retinal photoisomerase and two putative visual cycle proteins are expressed in whole brain of a primitive chordate. J. Comp. Neurol. 460: 180-190.
Nakayama, A., Satou, Y. and Satoh, N. 2002. Further characterization of genes expressed during Ciona intestinalis metamorphosis. Differentiation 70: 429-437.
Nishida, H. 2003. Spatio-temporal pattern of MAP kinase activation in embryos of the ascidian Halocynthia roretzi. Dev. Growth Differ. 45: 27-37.
Oda-Ishii, I. and Saiga, H. 2003. Genomic organization and promoter and transcription regulatory regions for the expression in the anterior brain (sensory vesicle) of Hroth, the otx homologue of the ascidian, Halocynthia roretzi. Dev. Dyn. 227: 104-113.
Ogasawara, M. and al., e. 2002. Gene expression profiles in young adult Ciona intestinalis. Dev. Genes Evol. 212: 173-185.
Oku, N., Matsunaga, S. and Fusetani, N. 2003. Shishijimicins A-C, novel enediyne antitumor antibiotics from the ascidian Didemnum proliferum. J. Amer. Chem. Soc. 125: 2044-2045.
Ooishi, S. 2002. Description of Botryllophilus sarsi, new species (Copepoda: Cyclopoida: Ascidicolidae), replacing B. brevipes Sars, 1921. J. Crust. Biol. 22: 819-833.
Ooishi, S. 2002. Redescription of Botryllophilus inaequipes Hansen, 1923 (Crustacea: Copepoda: Cyclopoida: Ascidicolidae). Proc. Biol. Soc. Wash. 115: 636-649.
Padma, P., Satouh, Y., Wakabayashi, K., Hozumi, A., Ushimaru, Y., Kamiya, R. and Inaba, K. 2003. Identification of a novel leucine-rich repeat protein as a component of flagellar radial spoke in the ascidian Ciona intestinalis. Mol. Biol. Cell 14: 774-785.
Parrinello, N., Cammarata, M., Vazzana, M. and Arizza, V. 2001. Immunological activity of ascidian hemocytes. In: Sawada, H., Yokosawa, H. and Lambert, C. C. (ed.), The Biology of Ascidians. Tokyo, Springer-Verlag, pp. 395-401.
Pemberton, A. J., Noble, L. R. and Bishop, J. D. D. 2003. Frequency dependence in matings with water-borne sperm. J. Evol. Biol. 16: 289-301.
Pennati, R., Groppelli, S., Sotgia, C., Zega, G., Pestarino, M. and De Bernardi, F. 2003. WAY-100635, an antagonist of 5-HT(1A) receptor, causes malformations of the CNS in ascidian embryos. Dev. Genes Evol. 213: 187-192.
Pennisi, E. 2002. Comparative genomics. Tunicate genome shows a little backbone. Science 298: 2111-2112.
Perez, L. J. and Faulkner, D. J. 2003. Bistratamides E-J, modified cyclic hexapeptides from the Philippines ascidian Lissoclinum bistratum. J. Nat. Prod. 66: 247-250.
Philp, R. B., Leung, F. Y. and Bradley, C. 2003. A comparison of the metal content of some benthic species from coastal waters of the Florida panhandle using high-resolution inductively coupled plasma mass spectrometry (ICP-MS) analysis. Arch. Environ. Contam. Toxicol. 44: 218-223.
Popov, A. M., Novikov, V. L., Radchenko, O. S. and Elyakov, G. B. 2002. The cytotoxic and antitumor activities of the imidazole alkaloid polycarpin from the ascidian Polycarpa aurata and its synthetic analogues. Dokl. Biochem. Biophys. 385: 213-218.
Proksch, P., Ebel, R., Edrada, R. A., Schupp, P., Lin, W. H., Sudarsono, Wray, V. and Steube, K. 2003. Detection of pharmacologically active natural products using ecology. Selected examples from indopacific marine invertebrates and sponge-derived fungi. Pure & Applied Chem. 75: 343-352.
Raftos, D. A., Robbins, J., Newton, R. A. and Nair, S. V. 2003. A complement component C3a-like peptide stimulates chemotaxis by hemocytes from an invertebrate chordate-the tunicate, Pyura stolonifera. Comp. Biochem. Physiol. A 134: 377-86.
Rocha, R. M. 2002. Trididemnum maragogi sp. nov. (Ascidiacea, Didemnidae) from Alagoas, northeastern Brazil. Revista Brasileira de Zool. 19: 1105-1110.
Rudi, A., Chill, L., Aknin, M. and Kashman, Y. 2003. Didmolamide A and B, two new cyclic hexapeptides from the marine ascidian Didemnum molle. J. Nat. Prod. 66: 575-577.
Sasakura, Y., Awazu, S., Chiba, S., Kano, S. and Satoh, N. 2003. Application of Minos, one of the Tc1/mariner superfamily transposable elements, to ascidian embryos as a tool for insertional mutagenesis. Gene 308: 11-20.
Sasakura, Y., Shoguchi, E., Takatori, N., Wada, S., Meinertzhagen, I. A., Satou, Y. and Satoh, N. 2003. A genomewide survey of developmentally relevant genes in Ciona intestinalis X. Genes for cell junctions and extracellular matrix. Dev. Genes Evol. 10: 10.
Satoh, N. 2003. The ascidian tadpole larva: comparative molecular development and genomics. Nat. Rev. Genet. 4: 285-95.
Satou, Y., Sasakura, Y., Yamada, L., Imai, K. S., Satoh, N. and Degnan, B. 2003. A genomewide survey of developmentally relevant genes in Ciona intestinalis V. Genes for receptor tyrosine kinase pathway and Notch signaling pathway. Dev Genes Evol 9: 9.
Schupp, P., Poehner, T., Edrada, R., Ebel, R., Berg, A., Wray, V. and Proksch, P. 2003. Eudistomins W and X, two new beta-carbolines from the micronesian tunicate Eudistoma sp. J. Nat. Prod. 66: 272-275.
Scotlandi, K., Perdichizzi, S., Manara, M. C., Serra, M., Benini, S., Cerisano, V., Strammiello, R., Mercuri, M., Reverter-Branchat, G., Faircloth, G., D'Incalci, M. and Picci, P. 2002. Effectiveness of ecteinascidin-743 against drug-sensitive and - resistant bone tumor cells. Clin. Cancer Res. 8: 3893-3903.
Segraves, N. L., Lopez, S., Johnson, T. A., A., S. S., Fu, X., Schmitz, F. J., Pietraszkiewicz, H., Valeriote, F. A. and Crews, P. 2003. Structures and cytotoxicities of fascaplysin and related alkaloids from two marine phyla - Fascaplysinopsis sponges and Didemnum tunicates. Tet. Lett. 44: 3471-3475.
Selander, E. and Tiselius, P. 2003. Effects of food concentration on the behaviour of Oikopleura dioica. Mar. Biol. 142: 263–270.
Shida, K., Terajima, D., Uchino, R., Ikawa, S., Ikeda, M., Asano, K., Watanabe, T., Azumi, K., Nonaka, M., Satou, Y., Satoh, N., Satake, M., Kawazoe, Y. and Kasuya, A. 2003. Hemocytes of Ciona intestinalis express multiple genes involved in innate immune host defense. Biochem. Biophys. Res. Commun. 302: 207-218.
Smith, S. E., Douglas, R., da Silva, K. B. and Swalla, B. J. 2003. Morphological and molecular identification of Saccoglossus species (Hemichordata: Harrimaniidae) in the Pacific Northwest. Can. J. Zool. 81: 133–141.
Spagnuolo, A., Ristoratore, F., Di Gregorio, A., Aniello, F., Branno, M. and Di Lauro, R. 2003. Unusual number and genomic organization of Hox genes in the tunicate Ciona intestinalis. Gene 309: 71-79.
Stoner, D. S., Ben-Shlomo, R., Rinkevich, B. and Weissman, I. L. 2002. Genetic variability of Botryllus schlosseri invasions to the east and west coasts of the USA. Mar. Ecol. Prog. Ser. 243: 93-100.
Takahashi, N., Li, W., Banerjee, D., Guan, Y., Wada-Takahashi, Y., Brennan, M. F., Chou, T. C., Scotto, K. W. and Bertino, J. R. 2002. Sequence-dependent synergistic cytotoxicity of ecteinascidin-743 and paclitaxel in human breast cancer cell lines in vitro and in vivo. Cancer Res. 62: 6909-6915.
Tincu, J. A., Menzel, L. P., Azimov, R., Sands, J., Hong, T., Waring, A. J., Taylor, S. W. and Lehrer, R. I. 2003. Plicatamide, an antimicrobial octapeptide from Styela plicata hemocytes. J. Biol. Chem. 278: 13546–13553.
Tiselius, P., Petersen, J. K., Nielsen, T. G., Maar, M., Møller, E. F., Satapoomin, S., Tönnesson, K., Zervoudaki, T., Christou, E., Giannakourou, A., Sell, A. and Vargas, C. 2003. Functional response of Oikopleura dioica to house clogging due to exposure to algae of different sizes. Mar. Biol. 142: 253–261.
Touratier, F., Carlotti, F. and Gorsky, G. 2003. Individual growth model for the appendicularian Oikopleura dioica. Mar. Ecol. Prog. Ser. 248: 141-163.
Tsuda, M., Kawakami, I. and Shiraishi, S. 2003. Sensitization and habituation of the swimming behavior in ascidian larvae to light. Zool. Sci. 20: 13-22.
Tsuda, M., Nozawa, K., Shimbo, K. and Kobayashi, J. 2003. Rigidins B-d, new pyrrolopyrimidine alkaloids from a tunicate Cystodytes species. J. Nat. Prod. 66: 292-294.
Tsuda, M., Sakurai, D. and Goda, M. 2003. Direct evidence for the role of pigment cells in the brain of ascidian larvae by laser ablation. J. Exp. Biol. 206: 1409-1417.
Ueki, T., Adachi, T., Kawano, S., Aoshima, M., Yamaguchi, N., Kanamori, K. and Michibata, H. 2003. Vanadium-binding proteins (vanabins) from a vanadiunm-rich ascidia Ascidia sydneiensis samea. Biochim. Biophys. Acta 1626: 43-50.
Vargas, C. A., Tönnesson, K., Sell, A., Maar, M., Møller, E. F., Zervoudaki, T., Giannakourou, A., Christou, E., Satapoomin, S., Petersen, J. K., Nielsen, T. G. and Tiselius, P. 2002. Importance of copepods versus appendicularians in vertical carbon fluxes in a Swedish fjord. Mar. Ecol. Prog. Ser. 241: 125–138.
Viracaoundin, I., Barnathan, G., Gaydou, E. M. and Aknin, M. 2003. Phospholipid FA from Indian Ocean tunicates Eudistoma bituminis and Cystodytes violatinctus. Lipids 38: 85-88.
Wada, S., Tokuoka, M., Shoguchi, E., Kobayashi, K., Di Gregorio, A., Spagnuolo, A., Branno, M., Kohara, Y., Rokhsar, D., Levine, M., Saiga, H., Satoh, N. and Satou, Y. 2003. A genomewide survey of developmentally relevant genes in Ciona intestinalis II. Genes for homeobox transcription factors. Dev Genes Evol 8: 8.
Weinreb, S. M. 2003. Lepadiformine: a case study of the value of total synthesis in natural product structure elucidation. Acc. Chem. Res. 36: 59-65.
Witman, J. D. and Smith, F. 2003. Rapid community change at a tropical upwelling site in the Galapagos Marine Reserve. Biodiv. & Conserv. 12: 25-45.
Yagi, K., Satou, Y., Mazet, F., Shimeld, S. M., Degnan, B., Rokhsar, D., Levine, M., Kohara, Y. and Satoh, N. 2003. A genomewide survey of developmentally relevant genes in Ciona intestinalis III. Genes for Fox, ETS, nuclear receptors and NFkappaB. Dev. Genes Evol. 13: 13.
Yoshida, M., Ishikawa, M., Izumi, H., De Santis, R. and Morisawa, M. 2003. Store-operated calcium channel regulates the chemotactic behavior of ascidian sperm. Proc. Natl. Acad. Sci. 100: 149-154.
Yund, P. O. and Stires, A. 2002. Spatial variation in population dynamics in a colonial ascidian (Botryllus schlosseri). Mar. Biol. 141: 955–963.
Zvyagintsev, A. Y., Sanamyan, K. E. and Koryakova, M.
D. 2003. The introduction of the ascidian Molgula manhattensis (De
Kay, 1843) into the Peter the Great Bay (Sea of Japan). Sessile Organisms