Gretchen and Charles Lambert
home page: http://depts.washington.edu/ascidian/
Number 67 December 2010
Thanks so much to all of you who sent in the many contributions to this issue; it is gratifying to know that AN is still an important resource. We also enjoy hearing from you. There are 115 new publications listed at the end of this newsletter, many abstracts from recent meetings, announcements of upcoming meetings in 2011, and much more! We hope you find it useful and interesting.
We spent most of June and July at the Friday Harbor labs where Gretchen continued her work on the Pacific distribution of the Atlantic Molgula citrina (Aquatic Invasions 5 (4): 369-378) and Charley worked on aspects of germinal vesicle breakdown in Boltenia villosa oocytes. In late July we joined a team of biologists on a rapid assessment survey for invasive marine species in New Hampshire, Maine, Mass. and Rhode Island. The RAS was organized by Judy Pederson and Jan Smith from Mass. Sea Grant and Coastal Zone Management. In September we attended the Invertebrate Fertilization meeting at the Friday Harbor Labs organized by Steve Stricker and Gary Wessels. Charley presented a paper on Boltenia GVBD. In October we joined a survey group from Oregon State University organized by John Chapman. On this RAS we investigated ascidians on solid substrates in Coos Bay, Newport Bay and elsewhere along the coast. We were surprised to see large numbers of Corella inflata in Coos Bay where we had never seen more than a few, and a large number of the Atlantic Molgula citrina. The newsletter is a bit late this time because December has taken us to Sacramento, California where Charley is undergoing medical treatment, but we are greatly enjoying our new granddaughter Alise.
*Ascidian News is not part of the scientific literature and should not be cited as such.
1. The next Ascidian Workshop will be held at the Panama Bocas del Toro Smithsonian Tropical Research Institute June 9-30, 2011.
Pan American Advanced Studies Institute (PASI): Advanced Tunicate Biology: Integrating Modern and Traditional Techniques for the Study of Ascidians
Notice that this is a 3 week workshop, which will include:
following link gives background information on the participating experts:
For more information, go to the following link:
and click on the Download PDF link (4.36mb).
2. From Ken Hastings,
The Sixth International Tunicate Meeting will be held in Montréal, Québec, Canada, at McGill University, July 3-7, 2011.
Abstracts may be submitted describing any aspect of tunicate research, such as developmental biology, regeneration, genomics, genetics, cell biology, physiology, neurobiology, biochemistry, allorecognition, evolution/systematics, ecology. Abstracts describing research on tunicates as invasive species are welcome as this will be a topic of special interest at this meeting (see #5 below from Mary Carman). In addition, abstracts describing research on other organisms that sheds light on deuterostome evolution and chordate origins are also welcome. Abstract submission details are at http://apps.mni.mcgill.ca/tunicate/.
Ken Hastings on behalf of the Program Committee Chris Cameron, Mary Carman, Jeff Davidson, Robert Lauzon, Alex McDougal, Tom Meedel, Ian Meinertzhagen, Yutaka Satou.
3. 7th Intl. Conference on Marine Bioinvasions will be held at the CosmoCaixa Science Museum, Barcelona, Spain, 23-25 August 2011.
Entitled Advances and Gaps in Understanding Marine Bioinvasions, the conference will encompass the following themes:
Development and Tests of Invasion Theory; Drivers of Invasibility; Patterns of invasion and spread at local, regional, and global scales; Impact of bioinvasions on ecosystem structure and function, including the biology and ecology of invasive species; New tools for identification, monitoring, risk assessment, and management
We encourage you to submit proposals for special sessions related to these themes to Jeb Byers (email@example.com); the deadline for submission is 06 December 2010. [The Ascidian News editors apologize for the lateness of this newsletter but if you have a proposal, please submit anyway.] Sessions may be a half or a full day long; please include a title and list of potential speakers. Dr. Gemma Quilez-Badia firstname.lastname@example.org
4. Very interesting recent news release from EastBayRI.com on Univ. of Rhode Island research showing effects of Didemnum vexillum on infaunal diversity and winter flounder nutrition on the Georges Bank, off New England, USA http://www.eastbayri.com/detail/140050.html Are Sea Squirts Flounder-Friendly?
5. From Noa Shenkar, Arjan Gittenberger, Gretchen Lambert, Marc Rius, Rosana Moreira da Rocha, and Billie J Swalla
NEW World Ascidiacea Database.
The World Ascidiacea Database has just gone online as a part of the World Register of Marine Species initiative. It contains all currently accepted scientific names for ascidians and is progressively extended to include all published combinations, allowing both specialists and non-specialists to find the currently accepted name of a certain species.
Some taxonomic groups are complemented with available literature and pictures.
6. From Mary Carman, Biology Dept., Woods Hole Oceanographic Institution, Woods Hole, MA email@example.com
The papers from the International Invasive Sea Squirt Conference III (held at Woods Hole MA April 2010) will be published in an upcoming issue of Aquatic Invasions. Guest editors Andrea Locke and Mark Hanson have been receiving manuscripts of work presented at the conference and are in the process of putting together another important collection of research papers.
A new session, Invasive Tunicate Ecology, has been added to the next scheduled International Tunicate Meeting. Jeff Davidson, University of Prince Edward Island, and Mary Carman will serve as co-chairs for the session. Please see Ken Hastings’ 6th ITM announcement above and call for abstracts for the meeting to be held at McGill University in July 2011.
7. My name is Gil Koplovitz and I will be finishing my PhD dissertation at the University of Alabama at Birmingham about chemical defenses in Antarctic and subtropical ascidians around July 2011. I am currently looking for post-doc opportunities with a suitable laboratory. My research interests include chemical ecology, taxonomy and biology of ascidians, polar ecology, marine biological invasions and molecular ecology. My CV can be found at https://sites.google.com/site/gilkop/ and my email is firstname.lastname@example.org . Tel. 205-934-8322
WORK IN PROGRESS
1. Dermatan sulfate in urochordate phylogeny: Order-specific sulfation pattern and the effect of [→4IdoA(2-Sulfate)β-1→3GalNAc(4-Ssulfate) β -1→] motifs in dermatan sulfate on heparin cofactor II activity.
Eliene O. K. Farias1, Paula Lima1, Cristina P. Vicente2, Xingfeng Bao3, Kazuyuki Sugahara4 and Mauro S. G. Pavăo1,2 email@example.com
1Laboratório de Tecido Conjuntivo, Hospital Universitário Clementino Fraga Filho and Programa de Glicobiologia, Instituto de Bioquímica Médica, Univ. Fed. do Rio de Janeiro, Rio de Janeiro, RJ 21941-913, Brasil. 2Instituto de Biologia, Universidade Estadual de Campinas, Săo Paulo, Brasil. 3Glycobiology Unit, Tumor Microenvironment Program, Cancer Research Center, Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA. 4Graduate Sch. of Life Sci., Hokkaido Univ., Sapporo 001-0021, Japan.
The present study aimed to investigate the structure of dermatan sulfate (DS) and its ability to potentiate heparin cofactor II during urochordate evolution. To approach that we compared the disaccharide composition of DS polymers obtained from ascidian species of the Orders, Stolidobranchia (considered more primitive) - Herdmania momus, Halocynthia roretzi, Halocynthia pyriformis and Styela plicata and Phlebobranchia (considered more evolved) - Phallusia nigra and Ciona intestinalis. The disaccharide analysis indicated high content of disulfated disaccharide units in the DSs from both Orders. Interestingly, the degree of DS sulfation decreased from the more primitive to the more evolved ascidians. Thus, 76%
of the disaccharide unities in the DSs from primitive stolidobranchia ascidians were disulfated, compared to 53% in the DSs from more evolved phlebobranchia ascidians. The sulfation pattern of the Urochordate DS disaccharides showed interesting results, pointing to an evident structural difference: DSs from evolved Phlebobranchia ascidians contain mainly 2,6-sulfated disaccharides, such as echinoderms and vertebrate. However, DSs from the more primitive stolidobranchia ascidians contain mostly 2,4-sulfated disaccharides. Examining the phylogenetic relations among them, we can observe an alteration from 2,4 disaccharide units [ΔUA(2S)-GalNAc(4S)] in Echinoderms to 2,6 disaccharide units [ΔUA(2S)-GalNAc(6S)] in Phlebobranchia, during Urochordate evolution. Furthermore, in ascidians evolution, we observed an event of evolutionary regression to a 2,4-sulfated DS in Stolidobranchia. Ascidians
DS with a high 2,4-sulfated disaccharide content showed high anticoagulant activity (H. momus and H. roretzi). Assays with C. intestinalis, a 2,6-rich DS, showed low anticoagulant activity. The analysis of the DS structure and anticoagulant activity through HCII indicates a clear correlation between the anticoagulant activity of DS and the presence of 2,4-sulfated units.
2. From Patrick Lemaire: Summary of the conclusions of the 1st International Workshop on Tunicate information systems, Nice, France, November 11-14, 2010
50 people, mainly PIs from Japan, Europe and the USA gathered to plan the future of databases in the tunicate fields. Model species represented included: solitary ascidians (Ciona intestinalis and C. savignyi, Halocynthia roretzi, Phallusia mammillata, Molgula occulta and oculata), colonial ascidians (Botryllus schlosseri, Botrylloides leachi) and larvaceans (Oikopleura dioica). The meeting, sponsored by AVIESAN (France, http://www.aviesan.fr/en) and the DOPAMINET FP7 EU network (http://www.dopaminet.eu/) had two aims: 1) Take census of the current and foreseeable needs of the community, 2) Organize the integration of the current systems to satisfy best the needs of the community.
The meeting was highly successful and led to a 3 yr plan with the aims of fusing together existing databases while a new, more comprehensive system is developed, which will include multiple data sets (genomic, anatomy, embryonic models), and cover solitary and colonial ascidians, as well as larvaceans/thaliaceans, and offer more powerful search interfaces.
The meeting website can be found at http://www.tunicate-portal.org/TunicateDBMeeting_website/. This site hosts a participant list, slides of most talks, and a summary of the main conclusions of the meeting. patrick.lemaire@CRBM.CNRS.FR , on behalf of all co-organizers (T. Endo, K. Hotta, K. Inaba, Y. Satou, T. De Tomaso) and Delphine DAUGA, Aniseed Curator Delphine.DAUGA@ibdml.univmed.fr
3. Correlating abiotic factors and genetic patterns in marine invasive species: Importance for their distribution and spread. Mari Carmen Pinedaa, Victor Ordońezb, Christopher McQuaidc, Susanna López-Legentila, Xavier Turond, Marc Riuse
a Dept. de Biologia Animal, Facultat de Biologia, Univ. de Barcelona (firstname.lastname@example.org) b Dept. de Genčtica, Facultat de Biologia, Univ. de Barcelona
c Department of Zoology and Entomology, Rhodes Univ. d Centre d’Estudis Avançats de Blanes, CSIC e Centre for Invasion Biology, Zoology Dept., Univ. of Cape Town
Since September 2010, I have been working at the Department of Zoology and Entomology, Rhodes University (Grahamstown, South Africa), where I am studying the relationship between physical factors (temperature, salinity and pollutant concentrations) and genetic structure of globally distributed invasive ascidian species. The introduced solitary ascidians Styela plicata and Microcosmus squamiger are good model species because they are globally distributed, their phylogeography is now well understood, they can inhabit polluted waters although their ontogenic stages are likely to be sensitive to pollution stress. Nevertheless, these species differ ecologically within the study area: S. plicata is not present at some localities where M. squamiger can be easily found, suggesting a different tolerance to some abiotic factors. S. plicata usually inhabits polluted environments (harbours and marinas), while M. squamiger can also be found in more undisturbed habitats. In addition, previous genetic studies using the mitochondrial marker COI found important differences between these two species: S. plicata has different genetic composition when comparing two populations (Port Elizabeth and Knysna) along the south coast of South Africa, while the phylogeography of M. squamiger shows no significant genetic differentiation between these two populations.
Individuals of S. plicata and M. squamiger have been collected from Port Elizabeth and Knysna and we have studied them using a variety of methods: 1) Adults were exposed to different conditions (temperature, salinity and environmentally realistic copper concentrations) and their stress protein (Hsp70) levels were analyzed using western blots; 2) In vitro fertilization was achieved and the effect of the same factors on gametes, fertilization, larvae survival, settlement and metamorphosis was assessed; 3) Sequences of the COI gene of each individual were obtained to correlate the different responses with genetic patterns. This work will be completed in the next few months and will provide important information on the role of stress tolerance for the distribution and spread of these widespread introduced species.
4. Does ascidian MIS activate a proteinase activated receptor?
Charles Lambert, Univ. of Washington Friday Harbor Labs, Friday Harbor WA email@example.com
At the end of oogenesis many large oocytes with large germinal vesicles (diploid nucleus) are ovulated to be stored in the ovary until spawning. In most ascidian species transfer of ovarian oocytes to sea water results in the onset of meiosis (germinal vesicle breakdown; GVBD). In most other deuterostomes the maturation inducing substance (MIS) is a product of the follicle cells that induces GVBD in the oocytes. In 1991 Sakairi and Shirai proposed that the ascidian MIS was a trypsin-like protease. Removal of the follicle cells or treatment with soy bean trypsin inhibitor or other protease inhibitors blocked GVBD. These findings were verified in Lambert 2005, 2008. In the latter paper I also showed that isolated follicle cells released trypsin activity in response to an increase in pH. Current studies indicate that the ovarian pH of Boltenia villosa is below pH 6.
By the early new century scientists recognized that under certain circumstances proteases could activate receptors by proteolytic cleavage (Steinhoff et al 2005). In some ways this is similar to classical ligand–receptor activation except that it was irreversible. These receptors were termed proteinase activated receptors (PARs). Currently 4 types of PARs are known, PARs 1,3 and 4 which are activated by thrombin and PAR 2 which requires trypsin and not thrombin for activation (Steinhoff et al 2005). The original 1991 paper of Sakairi and Shirai contains several experiments demonstrating irreversible kinetics and this past summer I carried out additional experiments with Boltenia villosa oocytes showing that activation by trypsin is irreversible and cannot be activated by thrombin. My current hypothesis is that MIS release is inhibited by the acidity of the ovary. Trypsin is released from the follicle cells when the oocytes reach SW (pH 8.2) which binds to and activates an oocyte surface PAR 2 receptor. This irreversibly causes GVBD without the possibility of dilution of the ligand from the transfer from the ovary to SW.
Lambert , C.C. 2005. Signaling pathways in ascidian oocyte maturation: Effects of various inhibitors and activators on germinal vesicle breakdown. Dev. Growth & Differ. 47: 265–272.
Lambert , C.C. 2008. Signaling pathways in ascidian oocyte maturation: The role of cyclic AMP and follicle cells in germinal vesicle breakdown. Dev. Growth & Differ. 50: 181–188.
Sakairi, K. and H. Shirai 1991. Possible MS production by follicle cells in spontaneous oocyte maturation of the ascidian, Halocynthia roretzi. Dev. Growth & Differ. 33(2): 155-162.
Steinhoff, M. et al. 2005. Proteinase-activated receptors: transducers of proteinase-mediated signaling in inflammation and immune response. Endocrine Reviews 26(1):1–43.
1. III Meeting Italian Ascidiologists, Palermo, September 2010
Immunolocalizzazione di un peptide antimicrobico nella tunica di Ciona intestinalis (Tunicata). Di Bella M. A.1, Fedders H.2, Leippe M. 2 , De Leo G. 1 firstname.lastname@example.org
1 Dipartimento di Biopatologia e Biotecnologie mediche e forensi. Univ. di Palermo, Palermo, Italy; 2 Dept. of Zoophysiology,. Univ. of Kiel, Kiel, Germany.
I tunicati, sono cordati considerati per la loro posizione filogenetica, modelli significativi nello studio sulla evoluzione del sistema immunitario. Essi mancano di immunitŕ adattativa, ma sono provvisti di un efficient sistema di risposta innata affidata sia a fattori umorali che cellulari.. Di recente č stata identificata nel data base EST dell’ascidia Ciona intestinalis una nuova famiglia di geni putativi di molecole antimicrobiche che č stato dimostrato, sono sintetizzate e accumulate in alcuni emociti. Il peptide sintetico (Ci-MAM-A) esercita
una potente attivitŕ antimicrobica sia nei riguardi dei batteri che contro Candida albicans. Considerato che nei Tunicati, il peculiare tessuto di rivestimento, la tunica, svolge diverse funzioni quail riparazione ferite, attivitŕ immunologiche ed escretorie, riconoscimento del non-self, abbiamo testato anticorpi specifici generati dal peptide sintetico Ci-MAM-A come antigene, sulla tunica di C. intestinalis sia in condizioni fisiologiche, che in condizioni di infiammazione indotta. La molecola naturale č stata localizzata all’interno di alcuni granulociti della tunica e anche nella matrice di essa. Questi dati confermano che interazioni complesse si stabiliscono tra la espressione di molecole antimicrobiche naturali e il coinvolgimento delle cellule durante le reazioni di difesa effettuate contro i microrganismi.
2. XVI Simposio Ibérico de Estudios de Biología Marina, Alicante, Spain, 6-10 September 2010.
a) Ascidian-Cyanobacteria Symbiosis. Susanna López-Legentila, Bongkeun Songb, Joseph R. Pawlikb, Xavier Turonc
aDept. of Animal Biology (Invertebrates), Univ. of Barcelona. 645 Diagonal Avenue, 08028 Barcelona, Spain (email@example.com)
bCenter for Marine Science, University of North Carolina Wilmington. 5600 Marvin K. Moss Lane, Wilmington NC 28409, USA
cCentre d’Estudis Avançats de Blanes (CEAB, CSIC), c/ Accés cala St Francesc, 14 17300 Blanes-Girona, Spain
Symbiotic interactions between ascidians and microbes are only beginning to be explored, with few studies employing the molecular approaches required to accurately assess marine bacterial biodiversity. The majority of ascidian-microbe studies have focused on species within the family Didemnidae that establish symbiotic relationships with unicellular cyanobacteria from the genera Prochloron (Prochlorales) and Synechocystis (Chroococcales). These photosynthetic symbionts provide the host ascidians with a green or red pigmentation and an array of secondary metabolites, some of which have promising pharmaceutical properties. We have reviewed the existent literature on ascidian-cyanobacteria symbiosis and added our own research to the field by characterizing the cyanobacterial population of the didemnind species Trididemnum solidum, Trididemnum aff. cyanophorum, and Lissoclinum fragile. We sequenced first a fragment of the16S rRNA of 771 bp, and then extended these sequences to 1491 bp by including the entire 16S-23S rRNA internal transcribed spacer (ITS). Blast searches in GenBank showed that the best match for the main symbiont of Trididemnum solidum and Trididemnum aff. cyanophorum was Prochloron didemni (98% identity, 97% coverage; and 98% identity, 94% coverage respectively). For Lissoclinum fragile Blast searches with the 16S rRNA fragment of its main symbiont showed that the closer match was the freshwater cyanobacteria Leptolyngbya badia (95% max. identity, 100% coverage); while Blast searches using the full length of our sequences returned both Acaryochloris marina (95% identity; 85% coverage) and Leptolyngbya badia (100% identity, 61% coverage) as best matches. Unlike Prochloron, which was originally described as an ascidian symbiont, there is no record of ascidian symbiosis with Leptolyngbia. More intriguing will be a symbiosis with a cyanobacteria closely related to A. marina. A. marina is known to be the only oxygenic photoautotroph that uses chl d as the predominant photosynthetic pigment, and has been found growing on biofilms beneath didemnid ascidians, and more recently at the base of the ascidian Cystodytes dellechiajei (Policytoridae). Electron transmission microscope (TEM) observations revealed the presence in the tunic and the larvae of L. fragilum of cyanobacteria similar in structure and size to A. marina, suggesting that the symbionts were vertically transmitted to the progeny and reinforcing the symbiotic character of this group. Finally, we point out that, as found for sponges, ITS gene sequences displayed much higher variability than 16S rRNA sequences, highlighting the utility of ITS sequences in determining the genetic diversity and host specificity of symbiont populations among ascidian species.
b) Introductions and expansions: genetic differentiation and phylogeographic patterns in the solitary ascidian Styela plicata. Mari Carmen Pineda a, Susanna López-Legentila, Xavier Turonb
aDept. de Biologia Animal, Facultat de Biologia, Univ. de Barcelona (firstname.lastname@example.org)
bCentre d’Estudis Avançats de Blanes, CSIC
Marine introductions have increased considerably during the last century and are threatening native biodiversity while interfering with shellfish aquaculture and other fishery activities. Styela plicata (Lesueur, 1823) is a solitary ascidian widely distributed in both tropical and temperate waters, although its origin remains unclear. The aim of this study was to infer the genetic structure and phylogeography of S. plicata on a worldwide scale and to assess the potential role of ship transport on the current distribution of this species. To address this question we have analyzed two genetic markers: a fragment of the mitochondrial gene Cytochrome Oxidase subunit I (COI) and a fragment spanning the intron of the nuclear gene Adenine Nucleotide Transporter/ADP-ATP Translocase (ANT). A total of 344 individuals for COI and 291 for ANT were obtained from 2 locations on the Mediterranean Sea, 6 from the Atlantic, 6 from the Pacific and 2 from the Indian Ocean. The nuclear marker had a higher gene diversity (mean 0.764) and nucleotide diversity (mean 0.029) than the mitochondrial marker (0.487 and 0.005, respectively). The Mediterranean populations showed the least diversity for both markers, while the Indian Ocean and Western Atlantic (ANT) and Western Atlantic (COI) had the highest diversity in terms of both haplotypes and nucleotides. For COI we found two well-supported groups of haplotypes (>3% of divergence) but no clear phylogenetic structure was retrieved with ANT. Genetic divergence was significant for many population-pairs, especially for COI, irrespective of the geographic distance among them (i.e., no evidence of isolation by distance). Our results suggest that S.plicata has been present in all the studied oceans for a long time, possibly through recurrent introduction events via maritime transport. The source of the species’ expansion could not be unambiguously identified, although overall the Western Atlantic populations were the most diverse. The Mediterranean Sea seems to have been colonized more recently, judging from the low diversity and number of private haplotypes (only one for each marker). Stochasticity of the introduction events is reflected in the uneven distribution of both COI haplotype groups, the significant differences in haplotype frequencies among many populations, and the fact that there are populations with an excess or deficit of homozygotes for ANT. Although this species has been mainly found in harbour and artificial platforms, the potential spread of its populations to natural substrates cannot be discounted. Further studies regarding the invasive potential of S.plicata on a smaller geographic scale are necessary, together with an in-depth characterization of its reproductive cycles, recruitment rates and growth patterns.
c) Reproductive cycle of the invasive ascidian Styela plicata in the NW Mediterranean Sea. Mari Carmen Pinedaa, Alba Muntadasa, Roger Esplugaa, Susanna López-Legentila, Xavier Turonb
aDept. de Biologia Animal, Facultat de Biologia, Univ. de Barcelona (email@example.com)
bCentre d’Estudis Avançats de Blanes (CEAB, CSIC)
ascidian Styela plicata (Lesuer,
1823) presents nowadays a wide distribution in both tropical and temperate
waters, being some of the main components of the fouling communities in most
harbours and marinas all around the world. The geographical origin of this
species is uncertain but at present it can be certainly considered as
introduced in most of its distributional range. Although this species has the
ability to modify natural communities and hence produce important economical
impacts, studies about the biological cycle of this species are scarce. Our aim
was to study the reproductive cycle of Styela
plicata in the NW Mediterranean and for that purpose on January 2009 we
started collecting monthly samples of this species at two localities within the
Catalan coast. These localities were situated in the interior of the harbours
of Vilanova i
Once in the
laboratory, the animals were measured (length, width and height) and after
removing the tunic and opening the mantle, the gonads were dissected. Wet and
dry weights of gonads and mantle were used to calculate a gonadosomatic index
(GSI). Moreover, one of the gonads on the right side of the animal was used to
assess the maturity state through histological sections. Simultaneously,
temperature and salinity of the water at both localities was registered, as
well as recruitment observed, growth and population structure in the Vilanova i
3. 81st Annual Meeting of the Zoological Society of Japan, Tokyo, Japan, 21-23 September 2010.
Organization of the neural complex of Symplegma sp. (viride?). Hiromichi Koyama, College of Nursing, Sch. of Medicine, Yokohama City Univ.
I examined the structure of the neural complex of Symplegma sp. by light microscopic sections of Technovit 7100. The ciliated funnel has a simple slit-like structure. The cytoplasm of the funnel cells with inward long cilia stains faintly with cresyl violet. This tendency has been seen in all species studied so far. The cerebral ganglion is ovoid, and has a maximum diameter of 90 μm and a maximum length of 120 μm. The cerebral ganglion consists of cellular cortex and fibrous medulla. Most of the somata of the neurons are small (about 5 μm in diameter), but several huge somata (more than 10 μm in diameter) are observed. The neural gland is small and located above the anterior part of the cerebral ganglion. The neural gland consists of the cells with a large vacuole. The dorsal strand does not form a dorsal organ like Botryllus schlosseri, which belongs to the same subfamily as Symplegma. The dorsal strand descends a little behind the cerebral ganglion, and expands. The dorsal strand cells are connected loosely near the caudal end of it, and some cells become free. This situation is similar to the terminal cells of the dorsal strand of Polyandrocarpa misakiensis, a colonial styelid ascidian. Just as the terminal cells of P. misakiensis, the terminal cells of Symplegma sp. have a large inclusion stained red with cresyl violet. (Prof. T. Nishikawa is identifying the material of this study right now.)
4. 7th Intl. Symposium on the Chemistry and Biological Chemistry of Vanadium, October 6-9, 2010, Toyama City, Japan (http://www.vanadiumseven.com/).
The symposium was chaired by Prof. Hitoshi Michibata, Hiroshima University, and two co-chairs, Prof. Kan Kanamori, University of Toyama, and Prof. Toshikazu Hirao, Osaka University. A special issue in Coordination Chemistry Reviews will be released in early 2011.
The following presentations were presented from Prof. Michibata's laboratory, Dept. of Biological Sci., Graduate School of Science, Hiroshima Univ., 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8526, Japan. firstname.lastname@example.org Asterisks indicate presenting author.
a) Molecular mechanism of the transport and reduction pathway of vanadium in ascidians.
*Tatsuya Ueki and Hitoshi Michibata. email@example.com
b) poster: Identification of genes regulated by excess vanadium ions in ascidians, focusing on redox and accumulation of metals. *Satoshi Kume, Tatsuya Ueki and Hitoshi Michibata. Dept. of Biological Sci., Graduate School of Science, Hiroshima Univ. The gold prize of Outstanding Poster Awards for Young Scientists was given to Mr. Satoshi Kume for this poster.
c) poster: Expressed sequence tag (EST) analysis of the intestine from the most vanadium-rich ascidian Ascidia gemmata, and their application to heavy metal biosorption system. *Setijono Samino, Tatsuya Ueki, and Hitoshi Michibata
d) poster: Measurement and comparison of V(V)-reductase activity of Vanabin family of a vanadium-rich ascidian Ascidia sydneiensis samea. *Tomoya Kimizu, Tatsuya Ueki and Hitoshi Michibata.
5. 5th National Conference and Expo on Coastal and Estuarine Habitat Restoration, Galveston, Texas, November 13-17, 2010
The impact of invasive tunicates in marine coastal eelgrass habitats in Massachusetts
Grunden, David W.1; Carman, M.R.2; Colarusso, P.3; Chintala, M.M.4; Blackwood, D.S.5
1Oak Bluffs Shellfish Dept., P. O. Box 1327, Oak Bluffs, MA 02557
2Biology Dept., Woods Hole Oceanographic Institution, Woods Hole, MA 02543
3US Environmental Protection Agency, 5 Post Office Square, Suite 100, Boston, MA 02109
4US Environmental Protection Agency, Atlantic Ecology Division, 27 Tarzwell Dr, Narragansett, RI 02882
5US Geological Survey, 360 Woods Hole Rd, Woods Hole, MA 02543
Non-native tunicates have been invading New England marine coastal environments during the past 30 years, and are now posing a potential threat to eelgrass. Tunicates (Ascidiacea) are invertebrate marine filter feeders that overgrow natural and artificial substrates. Eelgrass (Zostera marina) beds are cosmopolitan, productive, and high diversity habitats that provide many significant ecosystem services (e.g., support a trophic food web, stabilize sediments, serve as nursery grounds for commercially important fish and shellfish). The consequences of invasive tunicates in coastal habitats can be devastating. For example, Didemnum vexillum has caused economic loss for New England sea scallop fishermen offshore at Georges Bank and shellfish aquaculturists in near shore waters and caused ecological destruction in intertidal and subtidal benthic communities. In 2008 and 2009, after decades of monitoring eelgrass meadows in Massachusetts, we found Botrylloides violaceus on eelgrass throughout Major’s Cove in Sengekontacket Pond, B. violaceus, D. vexillum, and Diplosoma listerianum attached to eelgrass in the middle area of Lake Tashmoo, and Ascidiella aspersa, B. violaceus, and D. vexillum on eelgrass throughout Stonewall Pond on Martha’s Vineyard; D. listerianum on eelgrass in patches at the east entrance to the Cape Cod Canal; and B. violaceus and D. listerianum on eelgrass at Gloucester Harbor and Cape Ann in northern Massachusetts. In Massachusetts, the exotic tunicates A. aspersa and D. vexillum have adapted to utilizing eelgrass as substrate and B. violaceus and D. listerianum, previously known to use eelgrass as substrate elsewhere, have vigorously spread into eelgrass habitats. This is the first recorded occurrence of A. aspersa and D. vexillum to use eelgrass as substrate. However, it is unknown if these are isolated incidents or indicative of a future trend. Tunicates commonly occurred as small patches on outer eelgrass blades but in some cases, D. vexillum encapsulated the plants to such an extent that they could no longer naturally defoliate or release seed. Tunicates likely block sunlight from reaching the blade, inhibiting necessary photosynthesis. Tunicate growth on eelgrass blades where epiphytic algae would otherwise be present appears to inhibit herbivorous grazers. Invasive tunicates are probably negatively impacting eelgrass, however, their filtration capacity may have a positive impact on the ecosystem by consuming excess phytoplankton and bacteria from the water. Changes in the environment such as warming water temperatures, ocean acidification, and excess nutrients may contribute to the success of these invaders. Climate change has negatively impacted native tunicates thus enabling non-native tunicates that have a greater temperature tolerance. Warming sea temperatures are shifting species’ ranges due to species’ thermal tolerances. Continued monitoring of eelgrass is necessary to determine the full impact of invasive tunicates on eelgrass beds.
6. Proc. of Natl. seminar on Biodiversity Conservation & Management of Aquatic Resources (NSBCMA 2010), 9-10 December 2010
A survey on invasive ascidians in south coast of India
Jaffar Ali H A, Sivakumar V and Tamilselvi M, Directorate of Research and Extension (Fisheries), Tamilnadu Veterinary & Animal Sciences Univ., Thoothukudi, India.
Invasive ascidians are a dominant feature of many sessile marine fouling communities throughout the world and may have negative effects on species diversity. There are 12 major ports and numerous minor ports along the 7,500km long Indian coastline, which may act as gateways for marine bio-invasions. The distribution and impact of invasive ascidians in India are less well documented but unsparing problems are developing and hence the present study was aimed to document the invasive ascidians in the South Indian coastal waters. In this paper both literature data and results from the field surveys conducted at ten coastal areas of south India during 2008-2010 were utilized to compile a checklist of invasive ascidians along the southern coasts of India. A total of sixty one species of ascidians has been reported in the present study. Among these, fifty four ascidians have been believed to be invasive species, with mostly from Australian origin. Seven ascidians are found as established invasive. Their arrival has been mainly due to shipping and ballast waters and some that have arrived recently may have significant future impact. The discovery of several new records during the survey indicates that the rate of introductions has substantially increased over the last two decades. The impact of the established invasive ascidians on sessile invertebrate community in Tuticorin and Vizhinjam Bay has already noted and a prediction has also been made on bioinvasion.
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