ASCIDIAN NEWS*
Gretchen Lambert
12001 11th
Ave. NW,
206-365-3734 gretchen.lambert00@gmail.com
home page: http://depts.washington.edu/ascidian/
Number
69 June 2012
Thanks to all
of you who sent the many contributions for this issue. There are 145 new publications listed at the end
of this newsletter, many abstracts from recent meetings, announcements of
upcoming meetings in 2012 and 2013, and much more. I hope you find it
interesting.
*Ascidian News is not part of the scientific
literature and should not be cited as such.
1. From Gretchen: It
has been a busy year ascidiologically (and otherwise) for me. In February I
taught an ascidian identification workshop at the Moss Landing Marine Lab in
California, and in April I taught another one in Nelson, New Zealand, at the
Cawthron Institute, which concluded with a very interesting boat ride out into
the Marlborough Sounds to visit two green-lipped mussel farms, watch them being
harvested, and examine the lines for ascidian fouling. I continue to identify
ascidians for individuals and institutions. I greatly miss my other half
though.
It is now 1 year since Charles’s death June
1, 2011. You can find a biography of Charles, that I was invited to write, at http://onlinelibrary.wiley.com/doi/10.1002/mrd.21384/pdf
.
I set up two
scholarship funds in his name last year, and they have received a number of contributions.
I am very happy to report that the first awards have been made, to very
deserving students. If you would like to continue to honor his
memory, and his accomplishments as a researcher and teacher, your contributions
will be greatly appreciated.
a) http://depts.washington.edu/fhl/help_endowments.html
The Charles Lambert Memorial Endowment. Provides assistance to graduate
students for research and/or coursework at the Univ. of Washington Friday
Harbor Labs that includes cell or developmental biology of marine
invertebrates, or any aspect of ascidian biology. Charley and I met at the Labs in
1964, when we went there to take summer classes. Without scholarship help we
could not have attended, and we would not have met! We soon transferred to UW
where we continued our graduate work. Charley received his Ph.D. in 1970, and
we returned to the Labs many times for research and teaching. We have been very
dedicated to helping others attend this fine institution.
b) http://www.fullerton.edu/foundation/direct/nsm/nsm-scholarships.asp
The Charles Lambert Memorial Scholarship fund. Charley taught in the Calif.
State Univ. Fullerton Biol. Dept. for 28 years, was the campus Outstanding
Professor for 1986 and earned other awards as well.
We also set up an online photo album: http://charlesclambertmemorial.shutterfly.com/
. You can click on an album photo and then scroll through for larger images
with labels. There are many recent additions I hope you will enjoy viewing. You
can add a comment or photos if you wish; they will be much appreciated.
2. From
Hitoshi Sawada: Director, Sugashima Marine
Biological Laboratory, Sugashima, Toba, Japan. hsawada@bio.nagoya-u.ac.jp
The
International Symposium on the Mechanisms of Sexual Reproduction in Animals and
Plants, a joint meeting of the 2nd Allo-authentication meeting and the 5th
Egg-coat Meeting (MCBEEC), will be held November 12-16, 2012, in Nagoya,
Japan.
Deadlines for
registration and abstract submission: Early: July 31, 2012; Regular: August 31,
2012. http://allo-authentication.net/5thMCBEEC/index.html
The
official URL of the 4th Egg-coat (MCBEEC) meeting, which was held in 2004 in
Ise-Shima, Japan, was also up-loaded at the following website:
http://www.bio.nagoya-u.ac.jp/~SugashimaMBL/4thMCBEEC/index.html There is a photo album in this website.
Please enjoy.
3. Dr. Patricia Mather (published as Patricia Kott), passed away on
Wednesday 4 January 2012 in Brisbane, Australia. She published many monographs
on the ascidians of Australia and worked for many years at the Queensland
Museum in Brisbane. A press release can be seen at http://www.couriermail.com.au/ipad/obituary-patricia-mather/story-fn6ck8la-1226251460148
. A full length biography with photos is forthcoming in Memoirs of the
Queensland Museum - Nature 2012 56(1):237-255. In Memorium
Patricia Mather (née Kott) (1925–2012) by Peter J.F. Davie. (Peter.Davie@qm.qld.gov.au). http://www.qm.qld.gov.au/About+Us/Publications/Memoirs+of+the+Queensland+Museum
4. From Gretchen: I recently donated my large collection of worldwide
samples of Didemnum vexillum to Dr. Sarah Cohen at the Romberg Tiburon
Center for Environmental Studies, San Francisco State University. There are 70
samples in 95% ethanol from the east and west coasts of the U.S. including
Alaska, Pacific Canada, France, Netherlands, Ireland, Italy, New Zealand, and
Japan. There are also 42 samples in 10% buffered seawater formalin from most of
the same locations; these latter may ultimately be housed at the Calif. Academy
of Sciences in San Francisco. Anyone wishing to borrow or obtain a small sample
should contact Dr. Cohen, as this was the arrangement for the donation. sarahcoh@rtc.sfsu.edu
5. From Christina
Simkanin, Univ. of Victoria, British Columbia, Canada and Graduate Research
Fellow, Smithsonian Environmental Research Center, Edgewater, Maryland. csimkanin@gmail.com
I have been
working with Greg Ruiz's Marine Invasions Lab at the Smithsonian Environmental
Research Center on a database of the introduced marine and estuarine
invertebrate species in the continental U.S., Alaska and Hawaii (The National
Estuarine and Marine Exotic Species Information System - NEMESIS). The tunicate
portion of the database, which includes 30 species, was launched publicly at
the beginning of March http://invasions.si.edu/nemesis/browseDB/GroupSummary.jsp?GRP=Tunicates.
It provides information
on the invasion history and current distribution of each species, and
summarizes key information on the ecology and known impacts of each invader. I
hope it will be useful for some of your work. If you see something that you
think is an omission or mistake, please let me know.
6. From Gemma Quilez-Badia (gquilez@atw-wwf.org
), Barcelona, Spain:
a. The
next International Conference on Marine Bioinvasions will be held at the end of
August 2013 in Vancouver, Canada. We look forward to seeing you there and
plan to issue a call for special sessions this fall.
b. New journal, BioInvasions Records: http://www.reabic.net/journals/bir/2012/Issue1.aspx
This is an open access, peer-reviewed
international journal focusing on applied research on alien species and
biological invasions in aquatic and terrestrial ecosystems of Europe, North
America and other regions. The journal provides the opportunity of timely
publication of first records of aquatic and terrestrial invaders and other
relevant information needed for risk assessments and early warning systems.
Also, relevant technical reports and conference proceedings can be considered
for publication in this journal. We
welcome submissions to the journal and of course encourage you to use it as a
resource. Frances Lucy lucy.frances@itsligo.ie and Vadim Panov bir_editor@reabic.net, vepanov@gmail.com
7. From David Kipling, Cardiff University, UK. kiplingd@cardiff.ac.uk
One thing of
note this year is that there are now Facebook Groups for people interested in
Tunicates. There is a general Tunicata group, plus a NE Atlantic Tunicates
group. If you use Facebook do have a look and join!
[From the editor: the Tunicata listserv has
been ongoing for many years, is easy to join, and continues to be a valuable
means of communication: Tunicata email discussion forum
http://www.jiscmail.ac.uk/lists/tunicata.html]
8. From Noa Shenkar, Zoology Department,
Tel-Aviv University, Israel.
The
“Ascidiacea World Database of Marine Species” (WoRMS) http://www.marinespecies.org/ascidiacea/
is being updated and revised constantly. In order to keep it up to date please
e-mail PDF files of new species publications to noa.shenkar@gmail.com. Any comments and corrections are greatly
appreciated.
Congratulations to Noa! She won the Marie
Curie reintegration grant, 150,000 euros for 3 years! She will use it to
establish her new ascidian lab.
9. From Geoff
Nette, Director,
Independent Marine Bio-Chemistry Research Pty Ltd, Point Lookout, Queensland,
Australia (geoffnette@imbcr.com.au) .
Geoff reports the death of Dr. Silvia Scippa, of the Stazione
Zoologica Anton Dohrn in Naples, on April 4. “I had the extremely good fortune
to work in Naples with Silvia from 1996 to 1998. It was the marine taxonomists
of the19th century, Cuvier of course comes to mind, and a few intrepid
investigators of the blood chemistry of these enigmatic organisms [the
ascidians], such as Harless and Winterstein, who must surely have fired the
imagination of one Martin Henze at the Stazione Zoologica 'Anton Dohrn' at the
turn of the last century. His own studies precipitated a generous portion of
research into ascidian chemistry right through the twentieth century, all from
his discovery of the Vanadium Assimilation phenomenon in the blood of
ascidians, and in particular within the so called morula cells of Phallusia mammillata, or so we all
thought. Little question of the animal, big question of the blood cells!
After some seventy years of research into
the accumulation of vanadium, by groups all around the globe, it was
Professoressa Scippa and her stalwart mentor and colleague Professor Mario de
Vincentiis and their co-workers who laid to rest the question of which cell
type actually assimilated the vanadium - the Signet Ring Cell! It is a natural
phenomenon poorly understood and even now Silvia's research does not receive
the recognition her discovery deserves, even though it is reflective of her
lifelong dedication to the study of these animal. Thank you Silvia for
the opportunity... and I know this to be a sentiment echoed by too many
researchers to name individually right here and right now. Vale Professoresa.”
WORK
IN PROGRESS
1. From Juan Bellas, Centro Oceanografico de Vigo, Spain (juan.bellas@vi.ieo.es): Last year we were
granted the following patent in Spain: Procedure for assessing the efficacy of
antifouling compounds by using the larval settlement bioassay with ascidians. Inventors: Juan Bellas and
Ricardo Beiras. Application number: ES 2333573 B1. Award date: 01/04/2011.
Publication date: 04/05/2011. Priority country: Spain. Holder entity:
Universidade de Vigo. This invention is related to
the use of the ascidian larval settlement bioassay in assessing the efficacy of
antifouling compounds used to prevent undesirable growth of organisms on the
surface of boat hulls and other submerged artificial structures. This
biological assay is easy to carry out in the laboratory and presents a rapid
response (48 h), allowing to evaluate the effectiveness of antifouling
compounds before the application on surfaces submerged in the marine
environment. The ascidian larval settlement bioassay has been successfully
applied to the evaluation of the efficacy of the two most widely used
antifouling compounds, TBT and copper. This bioassay could be used by the
maritime industry in routine or pre-industrial evaluation of the efficacy of
new antifouling biocides. The invention is also applicable to environmental
risk studies and to the monitoring of the pollution caused by the use of
antifouling paints in port areas.
2. From Christian Sardet, BioMarCell
group UMR 7009 BioDev CNRS / Univ P. et M. Curie,
Villefranche sur Mer, France. csardet@gmail.com , http://www.biologymultimedia.com
I
retired last August but with emeritus status which allows me to continue
working in the Villefranche laboratory. I have participated actively in the
Tara Oceans Expedition and in that context started the Plankton Chronicles
project mixing art and science: http://www.planktonchronicles.org.
One episode (videos and photos) concerns larvaceans:
http://www.planktonchronicles.org/en/episode/new-larvaceans-their-houses-are-nets
I also pursue some research on the egg
cortex with Japanese colleagues and hope to complete a map of the egg cortex
and of its changes after fertilization.
3. Temporal and spatial variation in ascidian
settlement within fouling communities in Mission Bay, San Diego, California Brianna
M. Tracy and N. Reyns. Univ. of San Diego, Dept. of Marine
Science and Environmental Studies, San Diego, CA 92108. briannamtracy@gmail.com
Ascidians are ecologically important members
of fouling communities, and often dominant organisms on Southern California
docks. While previous studies have documented the presence of native and
non-indigenous ascidians in the region, generally little is known about their
spatial and temporal settlement patterns, and how such variability influences
population dynamics and overall fouling community development. The purpose of
this study was to examine weekly ascidian settlement on replicate PVC plates
deployed at five locations in Mission Bay, an urbanized estuary in San Diego,
CA. Sampling locations were selected to represent the range of environmental
conditions in Mission Bay. Settlement was related to weekly measures of sea
surface temperature, salinity, turbidity, and flow. While this study is
ongoing, our preliminary results suggest that settlement rates may be highest
at sites that receive greatest tidal flushing. Additionally, ascidian die-offs
have occurred within one week of increased freshwater exposure following rain
events. This study will provide valuable insight about population
dynamics in coastal environments, and will serve as a valuable comparison to
studies documenting ascidian settlement in other California regions.
1. California
State Univ. Biotechnology Symposium, January 7, 2012, Santa Clara, CA.
The Rho GTPase Cdc42
is a physiological switch in the integrin-triggered signaling pathway of ascidian
sperm activation. Bridgett
A. Santos, Lamar Blackwell, Katie Richards, Adilene Sandoval and Robert A. Koch,
Dept. of Biol. Sci., Calif. State Univ. Fullerton.
The phylogenetic proximity to vertebrates of
the sea squirt, Ascidia ceratodes, makes
it a good model for studying fertilization. Activation of ascidian sperm cell
to penetrate the egg
barriers
permitting fusion with the oocyte involves actin-dependent motility. In ascidians
sperm binds eggs via sperm â1-like
integrin triggering focal adhesion kinase to initiate an actin polymerization
signaling cascade known in non-sperm mammalian cell motility to require Rho
GTPases (Cdc42, Rac1, RhoA). In this study we
investigated ascidian sperm cell activation using constitutively active (ca) and dominant negative (dn)
Cdc42 to test its role in ascidian integrindependent signaling. Cdc42ca and Cdc42dn were presented to live sperm cells by
liposomal delivery and livecell permeabilization. Liposomes loaded with Cdc42
protein variants (1mg/ml) were tested against “empty” liposomes filled with
140mM KCl to mimic cytosol. Sperm cells that fused with Cdc42dn liposomes were subsequently challenged
with integrin-activating mAb12G10 (10μM). Live-cell permeabilization
was achieved by incubating sperm cells with octyl-betaglucopyranoside and were
then incubated with Cdc42 variants (1.33 nM) in an intracellular buffer. Cells
incubated with Cdc42dn will
be challenged with mAb12G10 as above. Sperm cells exposed to Cdc42ca liposomes exhibited 50% of maximum
activation, a percentage equivalent to mAb12G10-initiated activation, whereas
sperm cells incubated with empty liposomes showed %-activation equal to
naturally occurring spontaneous activation. Only a small
percentage (15%) of sperm cells previously exposed to Cdc42dn liposomes could be activated by
mAb12G10, thus exhibiting a 70% inhibition. Permeabilized live sperm
cells incubated with Cdc4ca were
activated at 106.2±30% (n=6) of maximum responsivity. That liposomal delivery
of Cdc42ca induced
sperm cell activation established a potential role for Cdc42. That
mAb12G10-initiated activation was inhibited by Cdc42dn showed that wild type Cdc42 is
necessary for the process. That infusion of Cdc42ca (1.33nM) induced activation of sperm
cells established that physiological levels are sufficient to switch-on the
process. We predict that exposure to Cdc42dn
and challenge with mAb12G10 will show
inhibition similar to that found in liposomal delivery experiments. We
conclude that Cdc42 is physiologically involved in the â1-like
integrin-initiated pathway of ascidian sperm cells activation.
2. Lower Invertebrate Symbiosis
With Microorganisms. 25 February to 2 March 2012, Tel Aviv
and Eilat, Israel.
a. A case of sponge-envy? An emerging picture of ascidian-associated bacterial communities.
Susanna
López-Legentil, Dept. of Animal Biology (Invertebrates), Univ. of Barcelona, Barcelona,
Spain. slopez@ub.edu
Sponges (Porifera) and ascidians (Chordata) have followed distinct
evolutionary trajectories, however, both taxa have
converged to similar ecological lifestyles. Ascidians resemble sponges as
sessile filter-feeding organisms that disperse by motile larva, contain mineral
spicules and produce defensive secondary metabolites. In addition, the few studies
investigating ascidian microbial diversity have suggested rich and diverse bacterial
communities in their tissues. Here, we review the current status of ascidian microbiology
and compare the diversity, composition and stability of the ascidian microbiota
with symbiotic bacteria in sponges. Similarly to sponges, the Proteobacteria (Alpha,
Gamma and Delta) appear to be the predominant bacterial group in ascidians, with
Actinobacteria and Cyanobacteria also present in many ascidian species. Other bacterial
phyla such Bacteroidetes, Chloroflexi, Spirochaetes and Verrucomicrobia are only
retrieved occasionally. Phylogenetic analyses indicate that most
ascidian-associated bacteria are closely related to sponge symbionts, with the
remaining microbes clustering with seawater and marine sediment bacteria. We
also present the first data on the temporal stability of ascidian bacterial
symbionts, by characterizing and monitoring the microbial community in the
Mediterranean ascidian Didemnum fulgens. D. fulgens harbors a
bacterial consortium typical of ascidians, including numerous
Proteobacteria, and a few
Cyanobacteria and Acidobacteria. The overall bacterial community in D. fulgens
had a distinct signature from the surrounding seawater and, as found for
Mediterranean sponges, was stable over
time. Finally, both sponges and ascidians have often been reported to form
obligate symbiotic relationships with cyanobacteria. Undoubtedly, the most
emblematic and well-studied symbiotic relationship in ascidians is with Prochloron
(Prochlorales) and Synechocystis (Chroococcales), a genus closely related
to Prochloron. Our own research on the cyanobacterial populations of
didemnid species from the Bahamas confirmed the prevalence of these symbionts,
with most of the photosymbionts belonging to these two cyanobacterial genera.
In addition, didemnid species from the genus Lissoclinum harbored unique
cyanobacteria related to Acaryochloris, which uses the recently
described chlorophyll d as a predominant photosynthetic pigment. Similar
to observations of Prochloron, Acaryochloris-like symbionts also
appear to be vertically transmitted to progeny. As described for sponges, the
photosymbiont communities investigated were mainly structured by host
phylogeny, with some evidence of structuring by sampling location. In
conclusion, results to date suggest that the overall bacterial diversity in
ascidians is similar to sponges, with symbiont communities mostly host-specific
and stable over time. However, some
compositional differences are evident, for example
at the cyanobacterial level, where ascidians mostly harbor symbionts from the
genus Prochloron and sponges from the genus Synechococcus.
b. Not so lower: Bacterial diversity in ascidians from
the Great Barrier Reef. Mari Carmen Pineda1, Nicole
Webster2, Xavier Turon3, Susanna López-Legentil1 1 – Animal Biology Dept., Univ. of
Barcelona, Barcelona, Spain ; 2 – Australian Institute of Marine Science
(AIMS), Townsville, Australia; 3 - Centre for Advanced Studies of Blanes (CEAB, CSIC), Blanes, Spain. mcpineda@ub.edu
Ascidians
or sea-squirts are sessile filter-feeding organisms within the sub-phylum
Tunicata. Similar to other marine invertebrates, ascidians are known to harbor
microbial symbionts but have received much less research attention than sponges and corals. Here, 19 species of ascidians from the
Great Barrier Reef (order Aplousobranchia n=15, Phlebobranchia n=2, and
Stolidobranchia n=21) were studied to characterize the bacterial diversity
hosted in their tunics. Denaturing gradient gel electrophoresis (DGGE) profiles
of 16S rRNA revealed a high diversity of microbes in most of the collected
ascidians. Multidimensional Scaling (MDS) plots based of banding pattern
similarities showed that Rhopalaea sp. and Eudistoma amplum had
bacterial profiles similar to seawater samples. The symbiont communities of the
Didemnidae species (Aplousobranchia) Lissoclinum badium and Lissoclinum
patella were somewhat set apart from the rest of the ascidians investigated
in the MDS plot, while replicates from the same species generally grouped
closely together. Amplification and sequencing of DGGE bands revealed 82
different OTU’s (99% sequence identity), with Proteobacteria (Alpha, Gamma and
Delta) roughly representing 75% of the recovered microbes. Actinobacteria and
Cyanobacteria were present in most (n=14) of the species, while Bacteroidetes,
Chloroflexi, Spirochaetes and Verrucomicrobia were unevenly distributed among
the ascidian hosts. Further in-depth analyses using massively parallel 16S rRNA
gene tag pyrosequencing are currently underway. Here, we demonstrated that like
other marine invertebrates, ascidians harbor rich microbial communities that
exhibit varying levels of diversity and host-specificity.
3. Ocean Sciences Meeting, Salt Lake City, Utah,
February 20-25, 2012.
Seasonal variability in settlement and abundance of the non-indigenous
ascidian, Botryllus schlosseri, in a
subarctic harbor. Kevin C. K.
Ma (kevin.ma@mun.ca), J. Ben Lowen, Don Deibel, and Cynthia H. McKenzie, Ocean
Sciences Centre, Memorial University of Newfoundland, St. John’s, Canada.
Population
dynamics remain enigmatic for the non-indigenous temperate ascidian, Botryllus
schlosseri, which was identified on the south coast of Newfoundland in
2006. This represents a unique opportunity to study B. schlosseri in
subarctic waters characterised by a short productive season. The seasonal
cycles of colony abundance and larval settlement rates were determined in
Arnold’s Cove, Newfoundland, by (1) deploying an experimental array of
artificial settlement plates and (2) surveying a transect of 140 wharf pilings
using HD video. Coincident with maximum seasonal temperatures, peak settlement
was at 30-40 m⁻²d⁻¹ in
September and peak cover of colonies was 3% in October. Settlement rate was
greater at 1 and 2.5 m than 4 m depths and on PVC in preference to aluminum and
wood substrates. These results have ecological, economic, and management
implications as they address competition of space in subtidal benthic
communities, potential threats to sustainable bivalve aquaculture, and the
implementation of monitoring and mitigation efforts.
4. 41st Benthic Ecology meeting, Norfolk, Virginia,
March 23-25, 2012.
Life-history constraints affecting invasion
success in Botryllus schlosseri. Lowen, Ben1(jlowen@mun.ca); Deibel, Don1; Ma,
Kevin1; McKenzie, Cynthia H.2; Thompson, Ray J.1 1Ocean
Sciences Centre, Memorial Univ. of Newfoundland, St. John's, Canada; 2Northwest Atlantic Fisheries
Centre, Dept. of Fisheries and Oceans, St. John's, Canada.
The reproductive success of ectotherms in
temperate seasonal environments is constrained by temperature limits for growth
and sexual reproduction. Botryllus
schlosseri (class Ascidiacea) is a temperate ectotherm that disrupts
benthic communities and mariculture operations. Its dispersal from temperate to
colder subarctic waters raises the question as to how it could adapt to an
increasingly short season for growth and reproduction, and a longer
overwintering period. To answer this question, we followed cohorts of marked
colonies in Arnold's Cove, Newfoundland. B.
schlosseri grew and reproduced within the thermal limits described in
studies of temperate populations. Consequently, temporal windows for growth and
reproduction were shorter by 3 months in colder sub-arctic waters than in
temperate waters, indicating that the life cycle was time constrained. Compared
with colonies in temperate waters, these time constraints selected for enhanced
early reproductive effort (during 1-3 cycles), thereby shortening generation
time at the expense of future growth and reproduction of the parent colony.
This strategy is adaptive, as fitness in ectotherms is maximized by their
producing as many generations as possible within a season. Coupled with high
overwintering survival in autumn born colonies, these results help explain the
persistence of temperate B. schlosseri
in sub-arctic waters.
5. VI Intl. Meeting of the Latin
American Society for Developmental Biology, Montevideo, Uruguay, 26-29 April
2012.
Limited sex but lots of buds in the
colonial chordates or Budding in colonial chordates. A.
Stefania Gutierrez, P. Endara, Billie J. Swalla, Federico D. Brown (Universidad de los Andes, Bogotá, Colombia). fedavidbrown@googlemail.com,
fd.brown46@uniandes.edu.co
Tunicates show a great plasticity in
propagation mechanisms. Solitary species only reproduce sexually, whereas
colonial species reproduce sexually by internal fertilization and brooding or
asexually by budding. Different adult tissues in colonial species generate new
clonal individuals. We study the transition of a solitary ancestral species
into several colonial species in the Styelidae, in which the evolution of
budding and regenerative abilities can be reconstructed. By the use of recent
molecular phylogenetic data, and direct observations of budding in different
Styelid colonial species, we demonstrate how more recent clades show a higher
integration in the development of zooids and buds, whereas more basal clades
show a higher independence in the development of their zooids. Botryllus and Botrylloides have long been known to regenerate complete bodies
from remnant colony vasculature and circulatory progenitor cells after complete
zooid removal. By stem cell marker expression and cell labeling studies we
provide further evidence for stem cell like features in circulatory blood cells
of the derived Botryllus and Botrylloides. Systemic
removal of buds or zooids in these species show synchrony or size defects in
the development of the colony, suggesting signaling between differentiated and
undifferentiated individuals. In contrast, zooid or bud removal in Symplegma rarely shows an effect in the
development of the colony upon ablation of either buds or zooids demonstrating
a higher independence in a more basal species. Additionally, we find that
vascular budding is the most common mechanism for asexual propagation in Symplegma, in contrast to palleal
(lateral epidermis of zooids) budding in Botryllus
and Botrylloides. Our results from
comparative studies in different species allow us to reconstruct the steps for
the evolution of coloniality in the chordates, in which a release of stem cells
into the vasculature allowed the evolution of vascular budding, and a higher
integration in the development of individuals in the colony allowed the
evolution of palleal budding. Mechanistic developmental changes that occurred
during the evolution of budding in colonial chordates set the framework for
understanding stem cell function in other animals, including vertebrates.
6. 8th Intl. Vanadium Symposium:
Chemistry, Biological Chemistry, & Toxicology will be held August 15-18, 2012 in
Crystal City, VA, USA. Dr. Tatsuya Ueki
will give a talk on Vanabins.
Older
meetings:
7. BIOLIEF 2011 – 2nd World Conference on Biological Invasions and
Ecosystem Functioning, Mar del Plata, Argentina, November 21-24, 2011.
Ascidians (Tunicata,
Ascidiacea) colonizing hard substrates at Patagonian harbours. Marcos Tatián, Cristian Lagger, Evangelina Schwindt. mtatian@com.uncor.edu
Harbours are primary
areas of invasion for invertebrate benthic marine species. Depletion of
succesional plates along 6 major harbours of Patagonia in Argentina allowed the
analysis of the ascidian community and the detection of those considered
invasive species.
8.
2011 Joint annual meeting, Italian Society of Immunology, Clinical Immunology
and Allergology (SIICA) and German Society for Immunology (DGfI) – Riccione
(Italy) September, 28 – October 1, 2011.
Immune
roles of a rhamnose-binding lectin from the colonial urochordate Botryllus schlosseri. Ballarin L., Dept.
of Biology, University of Padova, Padova, Italy. ballarin@bio.unipd.it
Differently
from gnatostome vertebrates, invertebrate chordates rely only on innate
immunity for immune surveillance and lectins exert a pivotal role in
invertebrate immune defence. Here, we describe the immune roles played by a
recently identified member of the rhamnose-binding lectin (RBL) family from a
basal chordate, the colonial ascidian Botryllus
schlosseri. B. schlosseri RBL
(BsRBL) can activate phagocytes through: (i) induction of their directional
movement towards the source of the molecule; (ii) modification of cytoskeleton,
required for shape changes; (iii) stimulation of the respiratory burst, and
consequent production of reactive oxygen species (ROS) with microbicidal
activity, including superoxide anions and peroxides; and (iv) increase in the
ability to phagocytose foreign particles. BsRBL also induces the synthesis and
release, by cytotoxic morula cells (MCs), of cytokines recognised by anti-ILla
and anti-TNFa antibodies. At high concentrations, BsRBL induces degranulation
of MCs and cell death consequent to the release of the cytotoxic enzyme phenoloxidase
into the medium. Results are consistent with the existence of cross-talk
between B. schlosseri immunocytes (phagocytes and MCs). In addition, a
three-dimensional model for BsRBL is presented.
THESIS ABSTRACTS
1.
Lauren Marie Stefaniak.
Didemnum vexillum: identity,
origin, and life history of an invasive ascidian. University
of Connecticut, 2012. Ph.D. thesis advisor Dr. Robert
Whitlatch. lauren.stefaniak@uconn.edu
Over the past forty years, an increasing number of previously unrecorded
populations of a colonial ascidian belonging to the genus Didemnum have been documented in most temperate coastal regions of
the world, impacting aquaculture operations, natural rocky habitats,
cobble/gravel substrates, and eelgrass beds.
Recent morphological analyses identified the populations as Didemnum vexillum Kott, 2002. However, because of incomplete historical
records and the numerous mis-identifications of this species, the native range
of D. vexillum has not been
conclusively known. Also, little is
known about the life history cycle, population biology, and native ecology of
the species. The goals of this
dissertation are four-fold. First,
support the morphological identification of D.
vexillum using molecular characters.
Second, use population genetics to determine the native region of this
now cosmopolitan species. Third,
describe observations on the distribution and ecology of D. vexillum in Japan, a region where there is a dearth of information
on the species. And fourth, quantify
aspects of D. vexillum’s life history
cycle to examine the relative importance of sexual and asexual pathways to
reproduction and dispersal of D. vexillum.
[Editor’s note: much of this work has been published or is in press and in
preparation.]
2. Studies on some ascidians of
Tuticorin coast [Phallusia nigra]. D. Shanmuga Priya, A.P.C. Mahalaxmi College for Women, Thoothukudi-628 002, India.
Ph.D thesis, Manonmaniam Sundaranar Univ., Tirunelveli;
advisors Drs. S. Gopalakrishnan and V.K. Meenakshi.
The scope of the present investigation was
to perform pharmacognostical and phytochemical determinations, antibacterial
and antifungal activities studies, pharmacological studies such as
anti-inflammatory, antipyretic, analgesic, anaesthetic and wound healing
activities of Phallusia nigra Sav. from Tuticorin coast, in addition to its macroscopic and
microscopic characters. The results of physico-chemical properties, the
fluorescence analysis of the various solvent extracts such as petroleum ether
(400 - 600C), benzene, chloroform, methanol and water are
discussed. Major phytochemical
constituents such as alkaloids, steroids, flavonoids, quinones, saponins and
anthraquinones are present in almost all the extracts. Thin layer chromatographic studies of
the various extracts of P. nigra were
carried out in different solvent systems using Silica gel ‘G’ plate. Paper
chromatographic studies were performed for aqueous extract. The fluorescing
spots were located using UV-fluorescence viewing cabinet (365 nm) and the Rf values of the spots were measured
and compared. GC-MS analysis of the methanolic extract of P. nigra identified eleven chemical constituents; the major are methyl 3-bromo-1-adamantaneacetate (24.65%), n-hexadecanoic
acid (24.45%), (Z)-11-hexadecen-1-ol, (17.64%) and 2,6-dimethyl-6-trifluoroacetoxyoctane
(9.82%). In HPTLC studies, six peaks were observed: gallic acid, ferulic
acid, caffeic acid and the flavonoids rutin, isoquercitrin and quercetin. A
maximum peak area (27773.44) corresponding to quercetin was observed with
221.89 μg/g
concentration. These
pharmacognostical and phytochemical characters of Phallusia nigra can be used as a diagnostic tool for the correct
identification of the animal species.
Anti-bacterial
and anti-fungal screening of extracts of P.
nigra were performed against five gram positive bacteria, Bacillus cereus, Bacillus subtilis, Bacillus
megaterium, Sarcina lutea and Staphylococcus
aureus, six gram negative bacteria, Escherichia
coli, Pseudomonas aeruginosa,
Klebsiella pneumonia, Salmonella typhi, Proteus mirabilis and Pseudomonas pyocyanus and three fungi, Candida albicans, Aspergillus niger and Saccharomyces cerevisiae. The
antibiotics ofloxacin and nystatin were used as standard for bacteria and fungi
respectively. Antimicrobial activity was measured by Disc Diffusion method.
Highly significant activity was noticed in methanol and water extracts against
both bacteria and fungi. The minimum inhibitory concentration (MIC) of the
methanolic extract of P. nigra is 2-3
µg/ml. Phallusia nigra displayed good
antimicrobial potential and showed inhibition over the growth of the test
organisms used. The methanolic extract of P.
nigra was also screened for acute and chronic anti-inflammatory activity (carrageenan
induced paw edema, cotton pellet granuloma). The reduction in paw volume was
highly significant in Group V treated with 150 mg/kg bw
followed by Group IV (100 mg/kg bw), indicating a dose dependent activity
compared to that of the standard drug, Indomethacin (10 mg/kg bw). A highly
significant inhibition in the antiproliferative activity of the granulomatous
tissue was observed with 150 mg/kg bw in chronic
inflammatory studies. Studies on biochemical parameters of the serum, liver and
exudates indicated lower lipid peroxide content and γ-glutamyl
transpeptidase activity. The crude extracts were observed to decrease the
increased acid and alkaline phosphatase activity and decrease the serum albumin
content during chronic inflammation. Methanolic extract of P. nigra was subjected to antipyretic and analgesic activity by
Brewer’s yeast-induced pyrexia method and Eddy’s hot plate and tail immersion
methods respectively. The extract, when administered at a dose of 150 mg/kg bw caused significant antipyretic activity by lowering the
body temperature at fourth hour when compared to that of the standard drug,
paracetamol (10 mg/kg bw). Groups treated with 100 mg/kg bw
and 150 mg/kg bw had highly significant analgesic activity in both the methods
when compared to that of the standard drug, diclofenac sodium (10 mg/kg bw).
Methanolic extract of P. nigra was subjected to anaesthetic activity by intracutaneous
wheal method. The extract, when administered at a dose of 10% and 15% caused
highly significant anaesthetic activity when compared to that of the standard
drug, xylocaine (1%). The mean sleeping time and percentage relaxation of
muscle was also highly significant in the extract treated groups compared to
that of the standard drug, aminobarbitone. The methanolic extract of P. nigra was
screened for wound healing activity (Incision wound model, Excision wound model
and dead space model). In excision and incision wound models, the wound
contraction was highly significant in Group V treated with 15% followed by
Group IV (10%) indicating a dose dependent activity compared to that of the
standard drug, framycetin sulphate (2%). Epithelialization period was also
found to be highly significant when compared with that of the standard, in both
models. In the dead space model, significant increase in the wet and dry
weight of the granulation tissues, tensile strength and hydroxyproline content
of the granulation tissue was observed in the animals treated with the
methanolic extract of P. nigra. The findings reveal the presence of
bioactive components in P. nigra
which can be important in the synthesis of new drugs. [some editorial
shortening]
NEW PUBLICATIONS
Aguirre,
J. D. and Marshall, D. J. 2012. Does genetic diversity reduce sibling
competition? Evolution 66: 94-102.
Albalat,
R., Marti-Solans, J. and Canestro, C. 2012. DNA methylation in amphioxus: from
ancestral functions to new roles in vertebrates. Brief. Funct.
Genomics Proteomics 11: 142-155.
Aplin,
L. M. and Cockburn, A. 2012.
Ecological selection and sexual dimorphism in the sooty
oystercatcher, Haematopus fuliginosus. Austral Ecol. 37:
248-257.
Atapattu,
S. S. 2009. The effect of two common reef organisms on the growth of the common
reef coral Acropora formosa. Mar. Biodiversity
Rec. 2: e61
Ballarin,
L., del Favero, M. and Manni, L. 2011. Relationships Among hemocytes, tunic cells, germ cells, and
accessory cells in the colonial ascidian Botryllus schlosseri. J,
Exp, Zool, B 316: 284-295.
Behrendt,
L., Larkum, A. W., Trampe, E., Norman, A., Sorensen, S. J. and Kuhl, M. 2012.
Microbial diversity of biofilm communities in microniches associated with the
didemnid ascidian Lissoclinum patella. ISME J. 6: 1222-1237.
Berna,
L., D'Onofrio, G. and Alvarez-Valin, F. 2012. Peculiar patterns
of amino acid substitution and conservation in the fast evolving tunicate Oikopleura
dioica. Molec. Phylogen.
& Evol. 62: 708-717.
Birdsey,
E. M., Johnston, E. L. and Poore, A. G. B. 2012. Diversity and cover of a sessile
animal assemblage does not predict its associated mobile fauna. Mar. Biol. 159:
551-560.
Bock,
D. G., Macisaac, H. J. and Cristescu, M. E. 2012. Multilocus genetic analyses
differentiate between widespread and spatially restricted cryptic species in a
model ascidian. Proc. R. Soc. B: Biol. Sci. 279: 2377-2385.
Bonnet,
N. Y. K. and Rocha, R. M. 2011.
The Ascidiidae (Ascidiacea: Tunicata) of coastal Brazil. Zool. Studies 50:
809-825.
Brunetti,
R. 2011. Fixation and description of a neotype for Polycyclus
renierii Lamarck, 1815 (Tunicata, Ascidiacea, Styelidae, Botryllinae).
Boll. Mus. civ. St. nat.
Venezia 62: 105-113.
Brunetti,
R. and Mastrototaro, F. 2012. Botrylloides pizoni, a new
species of Botryllinae (Ascidiacea) from the Mediterranean Sea. Zootaxa 3258:
28–36.
Bumbeer,
J. A. and Rocha, R. M. 2012.
Detection of introduced sessile species on the near shore
continental shelf in southern Brazil. Zoologia 29: 126–134.
Cahill,
P., Heasman, K., Jeffs, A., Kuhajek, J. and Mountfort, D. 2012. Preventing
ascidian fouling in aquaculture: screening selected allelochemicals for
anti-metamorphic properties in ascidian larvae. Biofouling 28: 39-49.
Cameron,
C. B. and Bishop, C. D. 2012. Biomineral ultrastructure,
elemental constitution and genomic analysis of biomineralization-related
proteins in hemichordates. Proc. R. Soc. B: Biol. Sci. in press.
Carbone,
M., Nunez-Pons, L., Paone, M., Castelluccio, F., Avila, C. and Gavagnin, M.
2012. Rossinone-related meroterpenes from the Antarctic ascidian Aplidium
fuegiense. Tetrahedron 68: 3541-3544.
Caro,
A. U., Guiñez, R., Ortiz, V. and Castilla, J. C. 2011. Competition between
a native mussel and a non-indigenous invader for primary space on intertidal
rocky shores in Chile. Mar. Ecol. Prog. Ser. 428: 177–185.
Chebbi,
N., Mastrototaro, F. and Missaoui, H. 2009. Occurrence of Polycarpa
kornogi in the Mediterranean Sea. Mar. Biodiversity Rec. 2:
1-3.
Chen,
J. S., Pedro, M. S. and Zeller, R. W. 2011. miR-124
function during Ciona intestinalis neuronal development includes
extensive interaction with the Notch signaling pathway. Development 138:
4943-4953.
Chow,
B., Wray, M., Villines, B., Pinnick, G., Sheets, E., Spaulding, J. and Cohen,
C. S. 2012. Experimental whole body regeneration among botryllid ascidian
species in San Francisco Bay. Integr. Comp.
Biol. 52: E225.
Cima,
F. and Ballarin, L. 2012.
Immunotoxicity in ascidians: Antifouling compounds alternative to organotins III . The case of copper(I) and
Irgarol 1051. Chemosphere epub April 27.
Claar,
D. C., Edwards, K. F. and Stachowicz, J. J. 2011. Positive and
negative effects of a dominant competitor on the settlement, growth, and
survival of competing species in an epibenthic community. J. Exp. Mar.
Biol. Ecol. 399: 130–134.
Cohen,
C. S., McCann, L., Davis, T., Shaw, L. and Ruiz, G. 2011. Discovery and
significance of the colonial tunicate Didemnum vexillum in Alaska.
Aquatic Invasions 6: 263-271.
Cole,
L. L. 2012. Diversity and distribution of Tunicata
(Urochordata) in Tobago. J. Life Sci. 6: 221-232.
Crean,
A. J., Monro, K. and Marshall, D. J. 2011. Fitness consequences of larval traits
persist across the metamorphic boundary. Evolution 65: 3079-3089.
Denker,
E. and Jiang, D. 2012. Ciona intestinalis notochord as
a new model to investigate the cellular and molecular mechanisms of
tubulogenesis. Semin. Cell Dev. Biol. 23:
308-319.
Dishaw,
L. J., Flores-Torres, J. A., Mueller, M. G., Karrer, C., Skapura, D. P.,
Melillo, D., Zucchetti, I., De Santis, R., Pinto, M. R. and Litman, G. W. 2012.
A basal chordate model for studies of gut microbial immune
interactions. Frontiers in Molecular Innate Immunity 3: 1-10.
Donia,
M. S., Fricke, W. F., Partensky, F., Cox, J., Elshahawi, S. I., White, J. R.,
Phillippy, A. M., Schatz, M. C., Piel, J., Haygood, M. G., Ravel, J. and
Schmidt, E. W. 2011. Complex microbiome underlying secondary
and primary metabolism in the tunicate-Prochloron symbiosis.
Proc. Natl. Acad. Sci. 108: E1423-1432.
Dumont,
C. P., Gaymer, C. F. and Thiel, M. 2011. Predation contributes to invasion
resistance of benthic communities against the non-indigenous tunicate Ciona
intestinalis. Biol. Invasions 13: 2023–2034.
Echevarria,
M., Naar, J. P., Tomas, C. and Pawlik, J. R. 2012. Effects of Karenia
brevis on clearance rates and bioaccumulation of brevetoxins in benthic
suspension feeding invertebrates. Aquatic Toxicol.
106-107: 85-94.
Ercolesi,
E., Tedeschi, G., Fiore, G., Negri, A., Maffioli, E., d'Ischia, M. and Palumbo,
A. 2012. Protein nitration as footprint of oxidative stress-related nitric
oxide signaling pathways in developing Ciona intestinalis. Nitric
Oxide: Biology and Chemistry epub 3
April 2012.
Fedonkin,
M. A., Vickers-Rich, P., Swalla, B. J., Trusler, P. and Hall, M. 2012. A new metazoan from
the Vendian of the White Sea, Russia, with possible affinities to the
ascidians. Paleontological J. 46: 1–11.
Fidler,
A. E., Holland, P. T., Reschly, E. J., Ekins, S. and Krasowski, M. D. 2012. Activation of a tunicate (Ciona intestinalis) xenobiotic
receptor orthologue by both natural toxins and synthetic toxicants.
Toxicon 59: 365-372.
Floerl,
O. and Inglis, G. J. 2005.
Starting the invasion pathway: the interaction between source
populations and human transport vectors. Biol. Invasions 7:
589–606.
Franchi,
N., Ferro, D., Ballarin, L. and Santovito, G. 2012. Transcription of genes
involved in glutathione biosynthesis in the solitary tunicate Ciona
intestinalis exposed to metals. Aquatic Toxicol. 114–
115: 14– 22.
Fujiwara,
S., Isozaki, T., Mori, K. and Kawamura, K. 2011. Expression and
function of myc during asexual reproduction of the budding ascidian Polyandrocarpa
misakiensis. Develop. Growth Differ. 53: 1004–1014.
Groener,
F., Lenz, M., Wahl, M. and Jenkins, S. R. 2011. Stress resistance in two colonial
ascidians from the Irish Sea: The recent invader Didemnum vexillum is
more tolerant to low salinity than the cosmopolitan Diplosoma listerianum.
J. Exp. Mar. Biol. Ecol. 409: 48-52.
Hall,
B. K. and Gillis, J. A. 2012.
Incremental evolution of the neural crest, neural crest cells
and neural crest-derived skeletal tissues. J. Anat. Epub.
Hanashima,
A., Ogasawara, M., Nomiya, Y., Sasaki, T., Bao, Y. and Kimura, S. 2012.
Genomic- and protein-based approaches for connectin (titin) identification in
the ascidian Ciona intestinalis. Methods 56: 18-24.
Haydar,
D. 2012. What is natural? The scale of cryptogenesis in the
North Atlantic Ocean. Diversity and Distributions 18: 101-110.
Hedge,
L. H. and Johnston, E. L. 2012.
Propagule pressure determines recruitment from a commercial shipping pier.
Biofouling 28: 73-85.
Hirose,
E. and Su, S.-W.
2011. A new record of a photosymbiotic ascidian from Kenting, Taiwan with a key
to the photosymbiotic species of the genus Diplosoma recorded in the
Western Pacific. Collection and Research 24: 83-86.
Hirose,
E., Nakashima, K. and Nishino, A. 2012. Is there intracellular cellulose in the appendicularian
tail epidermis? A tale of the adult tail of an invertebrate
chordate. Commun. Integr.
Biol. 4: 768-771.
Hirose,
E., Nozawa, A., Kumagai, A. and Kitamura, S. 2012. Azumiobodo hoyamushi gen. nov. et sp. nov. (Euglenozoa,
Kinetoplastea, Neobodonida): a pathogenic kinetoplastid causing the soft tunic
syndrome in ascidian aquaculture. Dis. Aquat. Organ. 97:
227-235.
Hopmann,
K. H., Sebestik, J., Novotna, J., Stensen, W., Urbanova, M., Svenson, J.,
Svendsen, J. S., Bour, P. and Ruud, K. 2012.
Ishijima,
S. 2012. Mechanical constraint converts planar waves into helices on tunicate
and sea urchin sperm flagella. Cell Struct. Funct. 37: 13-19.
Kanamori,
M., Baba, H., Hasegawa, N. and Nishikawa, T. 2012. Biological
characteristics, distinction and identification of Ascidiella aspersa
(Muller, 1776), as an alien ascidian in northern Japan. Sci. Rep.
Hokkaido Fisheries Res. Inst. 81: 151-156.
Kawamura,
K., Takakura, K., Mori, D., Ikeda, K., Nakamura, A. and Suzuki, T. 2012.
Tunicate cytostatic factor TC14-3 induces a polycomb group gene and histone
modification through Ca(2+) binding and protein
dimerization. BMC Cell Biol. 13:
Khalaman,
V. V. and Komendantov, A. Y. 2011.
Structure of fouling communities formed by Halichondria panicea (Porifera:
Demospongiae) in the White Sea. Russian J. Ecol. 42: 493-501.
Khalaman,
V. V., Mukhina, Y. I. and Komendantov, A. Y. 2011. The effects of the
excretory-secretory products of fouling organisms on settlement of larvae of
the sponge Halichondria panicea (Pallas, 1766) (Porifera: Demospongiae).
Russian J. Mar. Biol. 37: 494-500.
Kojima,
A. and Hirose, E. 2012.
Transmission of cyanobacterial symbionts during embryogenesis
in the coral reef ascidians Trididemnum nubilum and T. clinides
(Didemnidae, Ascidiacea, Chordata). Biol. Bull. 222: 63–73.
Koman,
J. S. and Tomanek, L. 2012. Proteomic analysis of hyposalinity stress in the
ascidian species Ciona savignyi and C. intestinalis. Integr. Comp. Biol. 52: E96.
Kostetsky,
E. Y., Velansky, P. V. and Sanina, N. M. 2012. Phospholipids of the organs and
tissues of echinoderms and tunicates from peter the
great bay (Sea of Japan). Russian J. Mar. Biol. 38: 64-71.
Kott,
P. and Esnal, G. 2009. Tunicata. In
New Zealand Inventory of Biodiversity. Gordon, D. ed.
Koyama,
H., Taneda, Y. and Ishii, T. 2012. The postbranchial digestive tract of the
ascidian, Polyandrocarpa misakiensis (Tunicata: Ascidiacea). 2. Stomach.
Zool. Sci. 29: 97-110.
Kremer,
L. P., Metri, R. and Rocha, R. M. 2011. Description of Sidneioides peregrinus sp. nov. (Tunicata: Ascidiacea: Polyclinidae): a possible exotic
species in the Atlantic Ocean. Zoologia 28: 784–788.
Lakhrach,
H., Hattour, A., Jarboui, O., Elhasni, K. and Ramos Espla, A. A. 2012. Spatial distribution and abundance of
the megabenthic fauna community in Gabes gulf (Tunisia, eastern Mediterranean
Sea). Medit. Mar. Sci. 13: 12-29.
Lee,
J., Currano, J. N., Carroll, P. J. and Joullie, M. M. 2012. Didemnins,
tamandarins and related natural products. Nat. Prod. Rep.
29: 404-424.
Lenz,
M., de Gama, B. A. P., Gerner, N. V., Gobin, J., Groener, F., Harry, A.,
Jenkins, S. R., Kraufvelin, P., Mummelthei, C., Sareyka, J., Xavier, E. A. and
Wahl, M. 2011. Non-native marine invertebrates are more tolerant towards
environmental stress than taxonomically related native species: Results from a
globally replicated study. Env. Res. 111:
943-952.
Lewis,
P. N., Bergstrom, D. M. and Whinam, J. 2006. Barging in: a
temperate marine community travels to the subantarctic. Biol. Invasions 8:
787–795.
Liberio,
M. S., Sooraj, D., Williams, E. D., Feng, Y. and Davis, R. A. 2011. Kingamide A, a new indole alkaloid
from the ascidian Leptoclinides kingi. Tet. Lett. 52:
6729-6731.
Lukowiak,
M. 2012. First Record of Late Eocene ascidians (Ascidiacea,
Tunicata) from southeastern Australia. J. Paleontology 86: 521-526.
Maeng,
S., Lee, J. H., Kim, G. J., Kim, S. H., Kwon, H. C., Shin, Y. K. and Sohn, Y.
C. 2012. Molecular and expression analysis of the farnesoid X
receptor in the urochordate Halocynthia roretzi. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 161: 189-196.
Makabe,
K. W. and Nishida, H. 2012. Cytoplasmic localization and reorganization in
ascidian eggs: role of postplasmic/PEM RNAs in axis formation and fate
determination. WIREs Dev. Biol. 1-18.
Maliska,
M. E. and Swalla, B. J. 2012.
Geographic range size and diversification in tailed and
tailless molgulid ascidians. Integr. Comp.
Biol. 52: E289.
Maliska,
M. E., Pierce, T., Hausch, P., Brown, C. T. and Swalla, B. J. 2012. Molgulid
ascidians show an early heterochronic shift in the expression of genes critical
for metamorphosis in other ascidians. Integr. Comp.
Biol. 52: E112.
McDougall,
A., Chenevert, J. and Dumollard, R. 2012. Cell-cycle control
in oocytes and during early embryonic cleavage cycles in ascidians.
Intl. Rev. Cell Mol. Biol. 297: 235-264.
McKindsey,
C. W., Landry, T., O'beirn, F. X. and Davies, I. M. 2007. Bivalve aquaculture and exotic
species: a review of ecological considerations and management issues. J.
Shellfish Res. 26: 281-294.
Meenakshi,
V. K., Gomathy, S. and Chamundeswari, K. P. 2012. GC-MS analysis of
the simple ascidian Microcosmus exasperatus Heller 1878. Intl. J.
ChemTech Res. 4: 55-62.
Meenakshi,
V. K., Gomathy, S. and Chamundeswari, K. P. 2012. Acute and
subchronic oral toxicity of Microcosmus exasperatus Heller, 1878.
J. Microbiol. Biotech. Res. 2: 94-98.
Meenakshi,
V. K., Paripooranaselvi., M., Gomathy, S. and
Chamundeswari, K. P. 2012. GC-MS analysis of the ethanolic
extract of Phallusia nigra Savigny, 1816. Proc. Natl. Conf.
Front. in Spectrosc. 2012 12-20.
Michibata,
H. and Ueki, T. 2012. High levels of vanadium in ascidians.
In Vanadium: Biochemical and Molecular Biological Approaches. Michibata, H., ed. Springer UK.
Monniot,
F. 2012. Some ascidians from the southern coast of Madagascar collected during
the “AtimoVatae” survey. Zootaxa 3197: 1-42.
Morris,
J. A. and Carman, M. R. 2012.
Fragment reattachment, reproductive status, and health indicators of the
invasive colonial tunicate Didemnum vexillum with implications for dispersal.
Biol. Invasions epub 22 April
Morton,
B. and Dinesen, G. E. 2011.
The biology and functional morphology of Modiolarca subpicta (Bivalvia:
Mytilidae: Musculinae), epizoically symbiotic with Ascidiella aspersa
(Urochordata: Ascidiacea), from the Kattegat, northern Jutland, Denmark. J.
Mar. Biol. Ass. U.K. 91: 1637-1649.
Murray,
C. C., Pakhomov, E. A. and Therriault, T. W. 2011. Recreational boating: a large
unregulated vector transporting marine invasive species. Diversity and
Distributions 17: 1161-1172.
Nakajo,
K., Nishino, A., Okamura, Y. and Kubo, Y. 2011. KCNQ1
subdomains involved in KCNE modulation revealed by an invertebrate KCNQ1
orthologue. J. Gen. Physiol. 138: 521-535.
Natale,
A., Sims, C., Chiusano, M. L., Amoroso, A., D'Aniello, E., Fucci, L., Krumlauf,
R., Branno, M. and Locascio, A. 2011. Evolution of anterior Hox regulatory
elements among chordates. BMC Evol. Biol. 11.
Nishida,
H. 2012. The maternal muscle determinant in the ascidian egg.
WIREs Dev. Biol. 1: 425–433.
Nishide,
K., Mugitani, M., Kumano, G. and Nishida, H. 2012. Neurula rotation determines
left-right asymmetry in ascidian tadpole larvae. Development 139:
1467-1475.
Nishitsuji,
K., Horie, T., Ichinose, A., Sasakura, Y., Yasuo, H. and Kusakabe, T. G. 2012. Cell lineage and cis-regulation for a
unique GABAergic/glycinergic neuron type in the larval nerve cord of the
ascidian Ciona intestinalis. Develop. Growth Differ. 54: 177-186.
Noda,
T. 2011. The maternal genes Ci-p53/p73-a and Ci-p53/p73-b regulate zygotic ZicL
expression and notochord differentiation in Ciona intestinalis embryos. Dev. Biol. 360:
216-229.
Obinata,
T. and Sato, N. 2012.
Comparative studies on troponin, a Ca2+-dependent regulator
of muscle contraction, in striated and smooth muscles of protochordates.
Methods 56: 3-10.
Occhipinti-Ambrogi,
A., Marchini, A., Cantone, G., Castelli, A. and al., e. 2011. Alien species along the Italian
coasts: an overview. Biol. Invasions 13: 215–237.
Ohkubo,
S. and Miyashita, H. 2012.
Selective detection and phylogenetic diversity of Acaryochloris
spp. that exist in association with didemnid ascidians and sponge.
Microbes & Environ. epub
Okamura,
K., Yamashita, R., Takimoto, N., Nishitsuji, K., Suzuki, Y., Kusakabe, T. G.
and Nakai, K. 2011. Profiling ascidian promoters as the
primordial type of vertebrate promoter. BMC Genomics 12 Suppl 3:
S7.
Ooishi,
S. 2011. Female and male Enterocola sydnii Chatton & Harant
(Copepoda: Cyclopoida: Ascidicolidae) living in compound ascidians. Proc. Biol.
Soc. Wash. 124: 358-377.
Paetzold,
S. C., Hill, J. and Davidson, J. 2012. Efficacy of high-pressure seawater spray
against colonial tunicate fouling in mussel aquaculture: inter-annual
variation. Aquatic Invasions epub: 1-12.
Pineda,
M. C., Turon, X. and López-Legentil, S. 2012. Stress levels over time in the
introduced ascidian Styela plicata: the effects of temperature and salinity
variations on hsp70 gene expression. Cell Stress and Chaperones epub:
1-10.
Plisson,
F., Conte, M., Khalil, Z., Huang, X. C., Piggott, A. M. and Capon, R. J. 2012. Kinase inhibitor scaffolds against
neurodegenerative diseases from a southern Australian ascidian, Didemnum
sp. ChemMedChem. 7: 983-990.
Rinkevich,
B., Douek, J., Rabinowitz, C. and Paz, G. 2012. The candidate
Fu/HC gene in Botryllus schlosseri (Urochordata) and ascidians'
historecognition--an oxymoron? Develop. and
Comp. Immunol. 36: 718-727.
Rius,
M., Turon, X., Ordóñez, V. and Pascual, M. 2012. Tracking invasion histories in the
sea: facing complex scenarios using multilocus data. PLoS One 7: e35815:
35811-35813.
Rob,
T., Ogi, T., Maarisit, W., Taira, J. and Ueda, K. 2011. Isolation of C(1)(1) compounds and a cyclopropane fatty acid from an
Okinawan ascidian, Diplosoma sp. Molecules 16: 9972-9982.
Robin,
F. B., Dauga, D., Tassy, O., Sobral, D., Daian, F. and Lemaire, P. 2011. Time-lapse imaging of live Phallusia embryos for creating 3D
digital replicas. Cold Spring Harb. Protoc. 2011: 1244-1246.
Robinson,
T. B., Griffiths, C. L., Tonin, A., Bloomer, P. and Hare, M. P. 2005. Naturalized populations of oysters,
Crassostrea gigas along the South African coast: distribution, abundance and
population structure. J. Shellfish Res. 24: 443–450.
Rocha,
R. M., Zanata, T. B. and Moreno, T. R. 2012. Keys for the
identification of families and genera of Atlantic shallow water ascidians.
Biota Neotropica 12: 1-35.
Rocha,
R. M., Kremer, L. P. and Fehlauer-Ale, K. H. 2012. Lack of COI variation for Clavelina
oblonga (Tunicata, Ascidiacea) in Brazil: Evidence for its human-mediated
transportation? Aquatic Invasions 7: 419-424.
Rocha,
R. M., Bonnet, N. Y. K., Baptista, M. S. and Beltramin, F. S. 2012. Introduced
and native Phlebobranch and Stolidobranch solitary ascidians (Tunicata:
Ascidiacea) around Salvador, Bahia, Brazil. Zoologia 29: 39-53.
Rodriguez-Martinez,
R. E., Jordan-Garza, A. G., Baker, D. M. and Jordan-Dahlgren, E. 2012. Competitive
interactions between corals and Trididemnum solidum on Mexican Caribbean
reefs. Coral Reefs 31: 571-577.
Saito,
T., Shiba, K., Inaba, K., Yamada, L. and Sawada, H. 2012. Self-incompatibility response induced
by calcium increase in sperm of the ascidian Ciona intestinalis. Proc.
Natl. Acad. Sci. 109: 4158-4162.
Sams,
M. A. and Keough, M. J. 2012.
Effects of pulse versus steady recruitment on sessile marine
communities. Oecologia epub:
Sasakura,
Y., Mita, K., Ogura, Y. and Horie, T. 2012. Ascidians as
excellent chordate models for studying the development of the nervous system
during embryogenesis and metamorphosis. Develop. Growth Differ. 54:
420-437.
Sasakura,
Y., Kanda, M., Ikeda, T., Horie, T., Kawai, N., Ogura, Y., Yoshida, R., Hozumi,
A., Satoh, N. and Fujiwara, S. 2012. Retinoic acid-driven Hox1 is required in
the epidermis for forming the otic/atrial placodes during ascidian
metamorphosis. Development 139: 2156-2160.
Satake,
H. and Sekiguchi, T. 2012. Toll-like receptors of deuterostome
invertebrates. Frontiers in Immunol. 3:
34.
Satheesh,
S. and Wesley, S. G. 2009.
Vertical distribution of macrofouling communities in
Kudankulam coastal waters, Gulf of Mannar (east coast of India). Mar.
Biodiversity Rec. 2:
Sato,
A., Satoh, N. and Bishop, J. D. D. 2012. Field identification of ‘types’ A and
B of the ascidian Ciona intestinalis in a region of sympatry.
Mar. Biol. epub: 1-9.
Schmidt,
E. W., Donia, M. S., McIntosh, J. A., Fricke, W. F. and Ravel, J. 2012. Origin and variation of tunicate secondary metabolites. J.
Nat. Prod. 75: 295-304.
Sekiguchi,
T., Ogasawara, M. and Satake, H. 2012. Molecular and functional characterization of cionin
receptors in the ascidian, Ciona intestinalis: the evolutionary origin
of the vertebrate cholecystokinin/gastrin family. J. Endocr. 213:
99-106.
Sensui,
N., Yoshida, M. and Tachibana, K. 2012. Role of Mos/MEK/ERK cascade and Cdk1 in Ca(2+) oscillations in fertilized ascidian eggs. Dev. Biol. epub:
Serafini,
L., Hann, J. B., Kultz, D. and Tomanek, L. 2011. The proteomic response of sea
squirts (genus Ciona) to acute heat stress: a global perspective on the
thermal stability of proteins. Comp. Biochem. Physiol. D Genomics Proteomics 6:
322-334.
Shaposhnikova,
T. G., Stolbovaia, A., Ponomartsev, N. V. and Podgornaia, O. I. 2011. Immuno- and histochemistry characteristics of morula and test cells
in three ascidian species. [In Russian with English abstract.].
Tsitologiya 53: 986-991.
Shenkar,
N. and Loya, Y. 2009. Non-indigenous ascidians (Chordata: Tunicata) along the
Mediterranean coast of Israel. Mar. Biodiversity Rec. 2: e166.
Shoguchi,
E., Fujie, M. and Hamada, M. 2011.
No chromosomal clustering of housekeeping genes in the marine chordate Ciona
intestinalis. Mar. Genomics 4: 151-157.
Simkanin,
C., Davidson, I. C., Dower, J. F., Jamieson, G. and Therriault, T. W. 2012. Anthropogenic
structures and the infiltration of natural benthos by invasive ascidians.
Mar. Ecol. epub:
Skidchenko,
V. S., Vysotskaia, R. U., Krupnova, M. and Khalaman, V. V. 2011. Effect of excretion-secretion
products of some fouling species on the biochemical parameters of blue mussel Mytilus
edulis L. (Mollusca: Bivalvia) in the White Sea. In Russian with English
abstract. Izv. Akad. Nauk. Ser. Biol. Nov.-Dec.: 670-683.
Smale,
D. A. and Childs, S. 2011. The occurrence of a widespread
marine invader, Didemnum perlucidum (Tunicata, Ascidiacea) in Western
Australia. Biol. Invasions epub 23 December.
Smith,
K. F., Thia, J., Gemmill, C. E. C., Cary, S. C. and Fidler, A. E. 2012.
Barcoding of the cytochrome oxidase I (COI) indicates a recent introduction of Ciona
savignyi into New Zealand and provides a rapid method for Ciona
species discrimination. Aquatic Invasions 7: in press.
Smith,
K. F., Stefaniak, L., Saito, Y., Gemmill, C. E. C., Cary, C. and Fidler, A. E.
2012. Increased
inter-colony fusion rates are associated with reduced COI haplotype diversity
in an invasive colonial ascidian Didemnum vexillum. PLoS
One 7: 1-8.
Sommer,
B., Harrison, P. L. and Scheffers, S. R. 2010. Aggressive colonial
ascidian impacting deep coral reefs at Bonaire, Netherlands Antilles.
Coral Reefs 29: 245.
Stefaniak,
L., Zhang, H., Gittenberger, A., Smith, K., Holsinger, K., Lin, S. and
Whitlatch, R. B. 2012.
Determining the native region of the putatively invasive
ascidian Didemnum vexillum Kott, 2002. J. Exp. Mar. Biol. Ecol. 422–423:
64–71.
Stolfi,
A., Wagner, E., Taliaferro, J. M., Chou, S. and Levine, M. 2011. Neural tube patterning by Ephrin, FGF
and Notch signaling relays. Development 138: 5429-5439.
Sugumaran,
M. and Robinson, W. E. 2012.
Structure, biosynthesis and possible function of tunichromes
and related compounds. Comp. Biochem. Physiol. B
Biochem. Mol. Biol. Epub.
Tamilselvi,
M., Sivakumar, V., Jaffar Ali, A. H. and Thilaga, R. D. 2011. Distribution of alien tunicates(ascidians) in Tuticorin Coast, India. World J. Zool. 6:
164-172.
Tatián,
M., Lagger, C., Demarchi, M. and Mattoni, C. 2011. Molecular phylogeny endorses the
relationship between carnivorous and filter-feeding tunicates (Tunicata,
Ascidiacea). Zoologica Scripta 40: 603–612.
Thompson,
H., Shaw, M. K., Dawe, H. R. and Shimeld, S. M. 2012. The formation and positioning of
cilia in Ciona intestinalis embryos in relation to the generation and
evolution of chordate left-right asymmetry. Dev. Biol. 364: 214-223.
Tolkin,
T. and Christiaen, L. 2012.
Development and evolution of the ascidian cardiogenic
mesoderm. Curr. Top.
Dev. Biol. 100: 107-142.
Treberg,
J. R., Stacey, J. E. and Driedzic, W. R. 2012. Vanadium accumulation in ascidian
coelomic cells is associated with enhanced pentose phosphate pathway capacity
but not overall aerobic or anaerobic metabolism. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 161: 323-330.
Tsukimoto,
M., Nagaoka, M., Shishido, Y., Fujimoto, J., Nishisaka, F., Matsumoto, S.,
Harunari, E., Imada, C. and Matsuzaki, T. 2011. Bacterial
production of the tunicate-derived antitumor cyclic depsipeptide didemnin B. J.
Nat. Prod. 74: 2329-2331.
Ueki,
T. and Michibata, H. 2011.
Molecular mechanism of the transport and reduction pathway of
vanadium in ascidians. Coord. Chem. Rev. 255: 2249-2257.
Valero-Jiménez,
C. A., Pérez-Portela, R. and López-Legentil, S. 2011. Characterization
of novel microsatellite markers from the worldwide invasive ascidian Styela
plicata. Conservation Genet. Resour. epub: 1-3.
Vance,
T., Lauterbach, L., Lenz, M., Wahl, M., Sanderson, R. A. and Thomason, J. C.
2009. Rapid invasion and ecological interactions of Diplosoma listerianum
in the North Sea, UK. Mar. Biodiversity Rec. 2: e59.
Veeman,
M. T., Chiba, S. and Smith, W. C. 2011. Ciona
genetics. Methods in Molec. Biol. 770:
401-422.
Vieira,
E., Duarte, L. F. L. and Dias, G. M. 2012. How the timing of predation affects composition and
diversity of species in a marine sessile community. J. Exp. Mar. Biol. Ecol. 412:
126 – 133.
Vierra,
D. A. and Irvine, S. Q. 2012.
Optimized conditions for transgenesis of the ascidian Ciona
using square wave electroporation. Dev. Genes &
Evol. 222: 55-61.
Vizzini,
A., Parrinello, D., Sanfratello, M. A., Salerno, G., Cammarata, M. and
Parrinello, N. 2012.
Inducible galectins are expressed in the inflamed pharynx of the ascidian Ciona
intestinalis. Fish & Shellfish Immunol. 32:
101-109.
Wang,
W. and Christiaen, L. 2012.
Transcriptional enhancers in ascidian development. Curr. Top. Dev. Biol. 98:
147-172.
White,
K. N. and Reimer, J. D. 2012. Commensal Leucothoidae
(Crustacea, Amphipoda) of the Ryukyu Archipelago, Japan. Part I:
ascidian-dwellers. Zookeys (163): 13-55.
Wong,
M. C. and Vercaemer, B. 2012.
Effects of invasive colonial tunicates and a native sponge on
the growth, survival, and light attenuation of eelgrass (Zostera marina).
Aquatic Invasions 7: 315-326.
Wong,
N. A., McClary, D. and Sewell, M. A. 2011. The reproductive ecology of the
invasive ascidian, Styela clava, in Auckland Harbour, New Zealand.
Mar. Biol. 158: 2775-2785.
Woznica,
A., Haeussler, M., Starobinska, E., Jemmett, J., Li, Y., Mount, D. and
Davidson, B. 2012. Title: Initial deployment of the cardiogenic gene regulatory
network in the basal chordate, Ciona intestinalis. Dev. Biol. in press.
Wyche,
T. P., Hou, Y., Vazquez-Rivera, E., Braun, D. and Bugni, T. S. 2012.
Peptidolipins B-F, antibacterial lipopeptides from an ascidian-derived Nocardia
sp. J. Nat. Prod. 75: 735-740.
Yadetie,
F., Butcher, S., Forde, H. E., Campsteijn, C., Bouquet, J. M., Karlsen, O. A.,
Denoeud, F., Metpally, R., Thompson, E. M., Manak, J. R., Goksoyr, A. and
Chourrout, D. 2012. Conservation and divergence of chemical
defense system in the tunicate Oikopleura dioica revealed by genome wide
response to two xenobiotics. BMC Genomics 13:
Yamazaki,
H., Wewengkang, D. S., Nishikawa, T., Rotinsulu, H., Mangindaan, R. E. and
Namikoshi, M. 2012.
Two new tryptamine derivatives, leptoclinidamide and (-)-leptoclinidamine B,
from an Indonesian ascidian Leptoclinides dubius. Mar. Drugs 10:
349-357.
Yoshida,
R. and Sasakura, Y. 2012. Establishment of enhancer detection lines expressing
GFP in the gut of the ascidian Ciona intestinalis. Zool. Sci. 29:
11-20.
Zvyagintsev, A. Y., Radashevsky, V. I.,
Ivin, V. V., Kashin, I. A. and Gorodkov, A. N. 2011. Nonindigenous
species in the far eastern seas of Russia. Russian J. Biol. Invasions 2:
164–182.