Figure 2.
Fully simplified variants. a, Filter binding assay (top) for streaks
of E. coli cells (bottom) bearing phagemids encoding wild type SH3,
the two fully simplified variants, and a negative control
(pBluescript) b, Sequences of fully simplified variants. Red: I,K,E,A,G. Black: residues
which did not tolerate simplification. Light blue: residues where simplification was
not attempted. c, Diagram showing the positions of simplified residues for
FP2 in the wild type SH3 structure (1SRL). Side chains involved in ligand
binding are displayed; the peptide ligand is in orange. The color scheme is as in
b.
Riddle, D., Santiago, J.,
Grantcharova, V. and Baker, D. (1997).
Functional rapidly folding proteins from simplified amino
acid sequences. Nature Structural Biology 4, 805-809.
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