High-resolution structural validation of the computational redesign of human U1A protein

TitleHigh-resolution structural validation of the computational redesign of human U1A protein
Publication TypeJournal Article
Year of Publication2006
AuthorsDobson, N., Dantas G., Baker D., & Varani G.
JournalStructure
Volume14
Issue5
Pagination847-56
Date Published2006 May
ISSN0969-2126
KeywordsAmino Acid Sequence, Collaborative Publication, Computer Simulation, Crystallography, X-Ray, Humans, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Protein Conformation, Ribonucleoprotein, U1 Small Nuclear, RNA-Binding Proteins, Thermodynamics
Abstract

Achieving atomic-level resolution in the computational design of a protein structure remains a challenging problem despite recent progress. Rigorous experimental tests are needed to improve protein design algorithms, yet studies of the structure and dynamics of computationally designed proteins are very few. The NMR structure and backbone dynamics of a redesigned protein of 96 amino acids are compared here with the design target, human U1A protein. We demonstrate that the redesigned protein reproduces the target structure to within the uncertainty of the NMR coordinates, even as 65 out of 96 amino acids were simultaneously changed by purely computational methods. The dynamics of the backbone of the redesigned protein also mirror those of human U1A, suggesting that the protein design algorithm captures the shape of the potential energy landscape in addition to the local energy minimum.

Alternate JournalStructure
AttachmentSize
dobson06A.pdf448.74 KB