Structural genomics of pathogenic protozoa: an overview

TitleStructural genomics of pathogenic protozoa: an overview
Publication TypeJournal Article
Year of Publication2008
AuthorsFan, E., Baker D., Fields S., Gelb M. H., Buckner F. S., Van Voorhis W. C., Phizicky E., Dumont M., Mehlin C., Grayhack E., Sullivan M., Verlinde C., Detitta G., Meldrum D. R., Merritt E. A., Earnest T., Soltis M., Zucker F., Myler P. J., Schoenfeld L., Kim D. E., Worthey L., Lacount D., Vignali M., Li J., Mondal S., Massey A., Carroll B., Gulde S., Luft J., Desoto L., Holl M., Caruthers J., Bosch J., Robien M., Arakaki T., Holmes M., Le Trong I., & Hol W. G. J.
JournalMethods in molecular biology
Volume426
Pagination497-513
Date Published2008
ISSN1064-3745
KeywordsAnimals, Crystallization, Crystallography, X-Ray, Genomics, Plasmodium, Primary Publication, Protozoan Proteins, Trypanosomatina
Abstract

The Structural Genomics of Pathogenic Protozoa (SGPP) Consortium aimed to determine crystal structures of proteins from trypanosomatid and malaria parasites in a high throughput manner. The pipeline of target selection, protein production, crystallization, and structure determination, is sketched. Special emphasis is given to a number of technology developments including domain prediction, the use of "co-crystallants," and capillary crystallization. "Fragment cocktail crystallography" for medical structural genomics is also described.

Alternate JournalMethods Mol. Biol.