Information for Baker Lab Members
  • Stopped-flow instrument maintenance and information
  • Journal Club/Group Meeting rotation
  • Decoy Test Results Subdirectory (limited access)
  • Lab Assistant Information

    Protocols

  • Chemically Competent Cells
  • Chemical Transformation
  • Colony Lift for Protein L
  • Colony Lift for SH3
  • DEAE DNA extraction from agarose gels
  • Electro Transformation Protocol
  • Electrocompetent Cells
  • Making Phagemids
  • Midi prep of DNA
  • Panning
  • Phage Amplification
  • Phage Precipitation/Purification
  • Protein Expression and Purification
  • Sequencing using the Big Dye Terminator
  • Tris-Tricine Buffered Electrophoresis for Protein Separation
  • Auto-Induction Media

    • Plasmid Maps

    phagemid constructs

  • pLt :phagemid construct w/ protein L B1 IgG binding domain, GeneVIII, tac promoter. ASCII
  • pLt_trp :phagemid construct w/ protein L (trp), GeneVIII, tac promoter. ASCII
  • pSH2t :phagemid construct w/ SH2, GeneVIII, tac promoter. ASCII
  • pSH3t :phagemid construct w/ src SH3, GeneVIII, tac promoter. ASCII

    Expression Constructs

  • pet15b: pdf file of Novagen pet15b plasmid map. Adobe Acrobat Reader needed to view pdf file.
  • pL_trp(pet15_delta_Mlu): pet15b construct with protein L (trp). ASCII
  • pet15_SH3: pet15b construct with src SH3 domain. ASCII

    • Tools

  • Oligonucleotide primer check (for mutagenesis).
  • Oligonucleotide primer generator (for mutagenesis).
  • DNA Primer Melt Calculator by Charlie Strauss
  • [GuHCl] and [Urea] Calculator
  • Biochemistry purchase request generator
  • Search past purchases

    • Home | Overview | Highlights | Publications | Members | Links

    http://depts.washington.edu/bakerpg