Stopped-flow maintenance and information
For more information go to the
Bio-Logic Stopped-flow instruments website
After using the stopped-flow instrument please follow the general
maintenance procedure below.
1. Make sure all solutions that leak from the syring barrels and
observation head are wiped and washed off thoroughly with water using
kimwipes.
2. Push 10 volumes of milli-Q water through the instrument from each
syringe.
3. Push 2 volumes of air through the instrument from each syringe.
4. Leave syringe pistons completely down.
5. Make sure syringe pistons are clean by wiping them with water using
kimwipes (leave no trace of GuHCl since it could scratch the barrels).
6. If you used the water circulator, empty the water in the stopped-flow
unit by:
a. Turning the water circulator off first.
b. Opening the 3-way valve located on the outflow tubing
to allow the water to flow back into the circulator.
DO
Make sure the instrument and bench is clean after you use them.
Filter your solutions before using them in the instrument
Log your usage and the condition of the instrument in the logbook.
Make a note in the logbook and/or notify David Kim (J559) if there are
any leaks or if the instrument is not working properly.
Remove your data on the PC (disk space is running out)
DON'T
Loosen and/or remove the observation head
Spill your solutions all over the place
Cleaning after extensive use (should be done weekly)
A 2% Hellmanex solution should be used to thoroughly clean the
instrument. Use gloves when handling the solution.
1. Rinse each syringe barrel with 2% hellmanex 2 times.
2. Follow the general cleaning procedure above.
HDS mixer position
The HDS stainless steel mixer should be placed with the four holes
pointing down away from the cuvette. It should always be in the observation
head (last mixer).
made Feb. 22, 1999; Baker Laboratory
Home |
Overview | Highlights |
Publications | Members | Information |
Links
http://depts.washington.edu/bakerpg