Research Themes:
Instrumentation, Imaging and Image-Guided Therapy
Biomaterials and Regenerative Medicine
Molecular and Cellular Engineering

Education

PhD (physical chemistry), University of California Berkeley, 1979
BS (chemistry), Oregon State University, 1975

Research Interests

• Surface Analysis
• Surface Modification
• Biomaterials

Contact Information

Department of Bioengineering
University of Washington
Box 355061
William H. Foege Building, Room N310G
Seattle, WA 98195-1750
Phone: 206-543-8094
Fax: 206-543-3778
E-mail
Web site: http://www.nb.engr.washington.edu

Research Description

The surface region of a biomaterial is the interface between that material and the biological environment. Thus, the surface structure and composition of a biomaterial mediates the biological reactions that occur when biomaterials are placed into the body. Our research at the National ESCA and Surface Analysis Center for Biomedical Problems is directed at obtaining detailed information about the surface composition and structure of biomaterials and the interaction of biomolecules with those biomaterials.

 

Recent advances have increased our ability to prepare complex, well-defined surface chemistries. The dimensions of lateral chemical variations are diminishing, the complexity of the molecules attached to the surface is increasing, and the manipulations of surface moieties are becoming more sophisticated. Surface analysis methods used include x-ray photoelectron spectroscopy, static time-of-flight secondary ion mass spectrometry, scanning force microscopy, near edge x-ray adsorption spectroscopy, sum frequency generation and surface plasmon resonance.

Current research projects include the characterization of model biomaterial systems (self-assembled monolayers, peptides, ordered proteins, biosensors, DNA microarrays, etc.), the development of chemical state imaging methods, and characterization of surface immobilized biomolecules. The objective of these research projects is to improve our understanding of the relationship between the surface properties of a biomaterial and the biological reactions occurring on that material.

 

 

 

Teaching Activities

• BIOEN 201: Tools for Bioengineering

Honors and Awards

1991-92 Chair, Pacific Northwest Chapter of the American Vacuum Society
1993-97 Chair, Surface Characterization & Modification Group, Society for Biomaterials
1996-97 Chair, Membership Committee, Society for Biomaterials
1994-99 Associate Editor, Plasmas and Polymers
1995-99 Secretary, Applied Surface Science Division, American Vacuum Society
1999 Local Arrangements Chair, American Vacuum Society
1999 Fellow, American Vacuum Society
2000 Program Chair, Biomaterials Interfaces Group, American Vacuum Society
2001 Chair, Biomaterials Interfaces Group, American Vacuum Society
2003 Excellence in Surface Science Award, Surfaces in Biomaterials Foundation
2004 Clemson Award for Basic Research, Society for Biomaterials
2004 Fellow, Biomaterials Science and Engineering
2004 Program Chair, American Vacuum Society International Symposium
2005-07 Board of Directors, American Vacuum Society

Selected Publications

• “Surface Characterization of the Extracellular Matrix Remaining after Cell Detachment from a Thermoresponsive Polymer,” H.E. Canavan, X. Cheng, D.J. Graham, B.D. Ratner and D.G. Castner, Langmuir, 21, 1949-1955, 2005.
• “Maximizing Information Obtained from Secondary Ion Mass Spectra of Organic Thin Films Using Multivariate Analysis,” M.S. Wagner, D.J. Graham, B.D. Ratner and D.G. Castner, Surface Science, 570, 78-97, 2004.
• “Analysis of Adsorbed Protein Films by Static ToF-SIMS,” M.S. Wagner and D.G. Castner, Applied Surface Science, 231-232, 366-376, 2004.
• "Methods for Generating Protein Molecular Ions in ToF-SIMS," S.L. McArthur, M.C. Vendettuoli, B.D. Ratner and D.G. Castner, Langmuir, 20, 3704-3709, 2004.
• "A Streptavidin Linker Layer That Functions After Drying," N. Xia, J. Shumaker-Parry, H. Zareie, C.T. Campbell and D.G. Castner, Langmuir, 20, 3710-3716, 2004.
• "Quantitative XPS and ToF-SIMS Characterization of the Components in DNA Microarrays," C.J. May, H.E. Canavan and D.G. Castner, Analytical Chemistry, 76, 1114-1122, 2004.
• "Characterization of Poly(L-Lysine)-graft-Poly(Ethylene Glycol) Assembled Monolayers on Niobium Pentoxide Substrates using ToF-SIMS and Multivariate Analysis," M. S. Wagner, S. Pasche, D. G. Castner, and M. Textor, Analytical Chemistry, 76, 1483-1492, 2004.
• "Covalent Coupling and Characterization of Supported Lipid Layers," S.L. McArthur, M.W. Halter, V. Vogel and D.G. Castner, Langmuir, 19, 8316-8324, 2003.
• "Denoising and Multivariate Analysis of ToF-SIMS Images," B. Wickes, Y. Kim and D.G. Castner, Surface and Interface Analysis, 35, 640-648, 2003.
• "Preserving the Structure of Adsorbed Protein Films for Time-of-Flight Secondary Ion Mass Spectrometry Analysis," N. Xia and D.G. Castner, J. Biomed. Mater. Res., 67A, 179-190, 2003.
• "ToF-SIMS: Techniques and Applications for the Characterization of Biomaterial Surfaces," A. Belu, D.J. Graham and D.G. Castner, Biomaterials, 24, 3635-3653, 2003.
• "Characterization of the Structure of Binary and Ternary Adsorbed Protein Films using Radiolabeling, ESCA and ToF SIMS," M.S. Wagner, T.A. Horbett and D.G. Castner, Langmuir, 19, 1708-1715, 2003.
• "Ultrasensitive Probing of the Protein Resistance of PEG Surfaces by Secondary Ion Mass Spectrometry," P. Kingshott, S.L. McArthur, H. Thissen, D.G. Castner and H.J. Griesser, Biomaterials, 23, 4775-4785, 2002.
• "ToF-SIMS Analysis of Conformational Changes in Adsorbed Protein Films," N. Xia, C.J. May, S.L. McArthur and D.G. Castner, Langmuir, 18, 4090-4097, 2002.
• "Interpretation of ToF-SIMS Spectra of Adsorbed Protein Films by Multivariate Pattern Recognition," M.S. Wagner, B.J. Tyler and D.G. Castner, Anal. Chem., 74, 1824-1835, 2002.
• "Surface Structure and Orientation of PTFE Films Determined by Experimental and FEFF8-Calculated NEXAFS Spectra," L.J. Gamble, B. Ravel, D.A. Fischer, and D.G. Castner, Langmuir, 18, 2183-2189, 2002.
• "Biomedical Surface Science: Foundations to Frontiers," D.G. Castner and B.D. Ratner, Surface Science, 500, 28-60, 2002.
• "Static ToF SIMS and XPS Characterization of Adsorbed Albunim and Fibronectin Films," C.D. Tidwell, D.G. Castner, S.L. Golledge, B.D. Ratner, K. Meyer, B. Hagenoff, and A. Benninghoven, Surface and Interface Analysis, 31, 724-733, 2001.
• "Surface Characterization of Mixed Self-Assembled Monolayers Designed for Streptavidin Immobilization," K.E. Nelson, L. Gamble, L.S. Jung, M.S. Boeckl, E. Naeemi, S.L. Golledge, T. Sasaki, D.G. Castner, C.T. Campbell, and P.S. Stayton, Langmuir, 17, 2807-2816, 2001.