David G. Castner

Joint Professor of Bioengineering and Chemical Engineering

Phone: (206)543-8094
Office: MolES 225

UW Bioengineering faculty David Casnter

Lab Website

How I am inventing the future of medicine

The objective of my research is to improve our understanding of the relationship between the surface properties of a biomaterial and the biological reactions occurring on that material.
Research Interests

Biomedical Surface Analysis
Surface & Interface Characterization
Surface Modification
Research Description

Our research is focused on determining in detail the surface structure and composition of a material, as well as the interaction of biomolecules with the surface of materials. Surface analysis methods used include x-ray photoelectron spectroscopy, static time-of-flight secondary ion mass spectrometry, scanning force microscopy, near edge x-ray adsorption spectroscopy, sum frequency generation and surface plasmon resonance. Current research projects include the characterization of model biomaterial systems (self-assembled monolayers, peptides, ordered proteins, biosensors, DNA microarrays, etc.), the development of 2D and 3D chemical state imaging methods for analysis of organic and biological samples, and characterization of surface immobilized biomolecules.

PhD, Physical Chemistry, University of California at Berkeley, 1979
BS, Chemistry, Oregon State University, 1975
Postdoc Information

Awards and Honors

2014 Rivière Prize, UK Surface Analysis Group
2013 Fellow, American Institute of Medical and Biological Engineering
2003-2015 Visiting Professor, Université Paris
2009-2011 President-Elect (09), President (10), Immediate Past President (11), American Vacuum Society
2005-2007 Board of Directors, American Vacuum Society
2004 Program Chair, American Vacuum Society International Symposium
2004 Clemson Award for Basic Research, Society for Biomaterials
2004 Fellow, Biomaterials Science and Engineering
2003 Excellence in Surface Science Award, Surfaces in Biomaterials Foundation
1999 Fellow, American Vacuum Society
UW Bioengineering Courses Taught

BIOEN 493/593: Advanced Surface Analysis
Selected Publications

“X-Ray Photoelectron Spectroscopy Investigation of the Nitrogen Species in Photoactive Perfluorophenylazide-Modified Surfaces,” G. Zorn, L.-H. Liu, L. Árnadóttir, H. Wang, L.J. Gamble, D.G. Castner and M. Yan, Journal of Physical Chemistry C, 118, 376-383, 2014.

“ToF-SIMS 3D Imaging of Native and Non-Native Species within HeLa Cells,” J. Brison, M.A. Robinson, D.S.W. Benoit, S. Muramoto, P.S. Stayton and D.G. Castner, Analytical Chemistry, 85, 10869-10877, 2013.

“Sodium Dodecyl Sulfate Adsorption onto Positively Charged Surfaces: Monolayer Formation with Opposing Headgroup Orientations,” S. Song, P. Koelsch, T. Weidner, M.S. Wagner and D.G. Castner, Langmuir, 29, 12710-12719, 2013.

“Low Energy Ion Scattering: Determining Overlayer Thickness for Functionalized Gold Nanoparticles,” A. Rafati, R. ter Veen and D.G. Castner, Surface and Interface Analysis, 45,1737-1741, 2013.

“Characterization of Sample Preparation Methods of NIH/3T3 Fibroblasts for ToF-SIMS Analysis,” M.A. Robinson and D.G. Castner, Biointerphases, 8, paper 15 (12 pages), 2013.

“SFG Analysis of Surface Bound Proteins: A Route Towards Structure Determination,” T. Weidner and D.G. Castner, Physical Chemistry Chemical Physics, 15, 12516-12524, 2013.

“Multivariate Analysis of ToF-SIMS Data from Multicomponent Systems: The Why, When, and How,” D.J. Graham and D.G. Castner, Biointerphases, 7, 2012, paper 49.

“NEXAFS Analysis of Individual Phenylalanine Orientations in Statherin Bound to Hydroxyapatite,” T. Weidner, M. Dubey, N.F. Breen, J. Ash, J. E. Baio, C. Jaye, D.A. Fischer, G.P. Drobny and D.G. Castner, J. American Chemical Society, 134, 8750-8753, 2012.

“ToF-SIMS Depth Profiling of Cells: Z-correction, 3D Imaging, and Sputter Rate of Individual NIH/3T3 Fibroblasts,” M.A. Robinson, D.J. Graham and D.G. Castner, Analytical Chemistry, 84, 4880-4885, 2012.

“Probing the Orientation of Electrostatically Immobilized Protein G B1 by Time of Flight Secondary Ion Spectrometry, Sum Frequency Generation and Near-edge X-Ray Adsorption Fine Structure Spectroscopy,” J.E. Baio, T. Weidner, L. Baugh, L.J. Gamble, P.S. Stayton and D.G. Castner, Langmuir, 28, 2107-2112, 2012.

“Exploring the Surface Sensitivity of ToF-SIMS by Measuring the Implantation and Sampling Depths of Bin and C60 Ions in Organic Films,” S. Muramoto, J. Brison and D.G. Castner, Analytical Chemistry, 84, 365-372, 2012.

“ToF-SIMS Analysis of Adsorbed Proteins: Principal Component Analysis of the Primary Ion Species Effect on the Protein Fragmentation Patterns” S. Muramoto, D.J. Graham, M.S. Wagner, T.G. Lee, D.W. Moon and D.G. Castner, Journal of Physical Chemistry C, 115, 24247-24255, 2011.

“Simulation and Modeling of Self-Assembled Monolayers of Carboxylic Acid Thiols on Flat and Nanoparticle Gold Surfaces,” S. Techane, D.R. Baer and D.G. Castner, Analytical Chemistry, 83, 6704-6712, 2011.

“Multi-technique Characterization of Self-assembled Carboxylic Acid Terminated Alkanethiol Monolayers on Nanoparticle and Flat Gold Surfaces,” S.D. Techane, L.J. Gamble and D.G. Castner, Journal of Physical Chemistry C, 115, 9432-9441, 2011.

“Structure and Order of Phosphonic Acid-Based Self-Assembled Monolayers on Si(100),” M. Dubey, T. Weidner, L.J. Gamble and D.G. Castner, Langmuir, 26, 14747-14754, 2010.

“A Sum Frequency Generation and Solid-State NMR Study of the Structure, Orientation and Dynamics of Polystyrene-Adsorbed Peptides,” T. Weidner, N.F. Breen, K. Li, G.P. Drobny and D.G. Castner, Proceedings of the National Academy of Sciences, 107, 13288-13293, 2010.


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