Project Description

Joint A. Bruce Montgomery Professor of Chemistry, Professor of Bioengineering
Phone: (206)543-1655
Office: Bagley 204


I am developing new technologies to address pressing needs in biomedicine.
Advance optical instrumentation
Single-molecule studies
Single-cell biology
Oncology; Synaptic Function
My lab is focused on developing new methods for probing complex biological processes at the single-cell and single-molecule level, and on applying these new techniques for addressing pressing biological problems.
Ph.D in Chemistry, Stanford University, 1998
B.A. in Neurobiology, B.S. in Chemistry, University of California at Berkeley, 1993
Postdoctoral Fellow, Harvard University, 2000
2012 Elected AAAS Fellow
2011 UW Presidential Entrepreneurial Faculty Fellow
2010 Analytical Chemistry Young Innovator Award
2009 Pittcon Achievement Award
2007 American Chemical Society National Fresenius Award
2005 Alfred P. Sloan Fellow
2004 McKnight Technological Innovations in Neuroscience Award
2003 Keck Distinguished Young Scholar in Biomedical Research
2002 NIH Cutting-Edge Technology in Basic Research Award
2002 National Science Foundation Career Award
2000 Research Corporation’s Research Innovation Award
2000 Dreyfus New Faculty Award
J. P. Shelby, D.S.W. Lim, J.S. Kuo, D.T. Chiu (2003) “Generation of ultra-high radial accelerations in microvortices” Nature 425, 38.

B. Sun and D. T. Chiu (2003) “Spatially and temporally resolved delivery of stimuli to single cells” J. Am. Chem. Soc. 125, 3702–3703.

J.P. Shelby, J. White, K. Ganesan, P.K. Rathod, D.T. Chiu (2003) “A microfluidic model for single-cell capillary obstruction by Plasmodium falciparum-infected erythrocytes” Proc. Natl. Acad. Sci. U.S.A. 100, 14618–14622.

M. He, J.S. Edgar, G.D.M. Jeffries, R.M. Lorenz, J.P. Shelby, D.T. Chiu (2005) “Selective encapsulation of single cells and subcellular organelles into picoliter and femtoliter-volume droplets” Anal. Chem. 77, 1539–1544.

G.D.M. Jeffries, J.S. Kuo, D.T. Chiu (2007) “Dynamic modulation of chemical concentration in an aqueous droplet” Angew. Chem. Int. Ed. 46, 1326-1328.

G.D.M. Jeffries, J.S. Edgar, Y. Zhao, J.P. Shelby, C. Fong, D.T. Chiu (2007) “Using polarization-shaped optical vortex traps for single cell nanosurgery” Nano Lett. 7, 415-420.

S.A. Mutch, B. Fujimoto, C.L. Kuyper, J.S. Kuo, S.M. Bajjalieh, D.T. Chiu (2007) “De-convolving single-molecule intensity distributions for quantitative microscopy measurements” Biophys. J. 92, 1-18.

Y. Zhao, J.S. Edgar, G.D.M. Jeffries, D. McGloin, D.T. Chiu (2007) “Spin-to-orbital angular momentum conversion in a strongly focused optical beam” Phys. Rev. Lett. 99, 073901.

J.S. Edgar, G. Milne, Y. Zhao, C.P. Pabbati, D.S.W. Lim, D.T. Chiu (2009) ”Droplet compartmentalization of chemical separations” Angew. Chem. Int. Ed. 48, 2719-2722.

D.T. Chiu & R.M. Lorenz (2009) “Chemistry and biology in femtoliter and picoliter volume droplets” Accounts of Chem. Res. 42, 649-658.

D.E. Cohen, T. Schneider, M. Wang, D.T. Chiu (2010) “Self-digitization of sample volumes” Anal. Chem. 82, 5707-5717.

C. Wu, T. Schneider, M. Zeigler, J. Yu, P.G. Schiro, D.R. Burnham, J.D. McNeill, D.T. Chiu (2010) “Bioconjugation of ultrabright semiconducting polymer dots for specific cellular targeting” J. Amer. Chem. Soc. 132, 15410-15417.

C. Wu, Y. Jin, T. Schneider, D. Burnham, and D.T. Chiu (2010) “Ultra-bright and bio-orthogonal labeling of cellular targets using semiconducting polymer dots and click chemistry” Angew. Chem. Int. Ed. 49, 9436-9440.

K.A.D. Gregersen, Z.B. Hill, J.C. Gadd, B.S. Fujimoto, D.J. Maly, D.T. Chiu (2010) “Intracellular Delivery of Bioactive Molecules using Light-Addressable Nanocapsules” ACS Nano 4, 7603-7611.

S.A. Mutch, P. Kensel-Hammes, J.C. Gadd, B.S. Fujimoto, R.W. Allen, P.G. Schiro, R.M. Lorenz, C.L. Kuyper, J.S. Kuo, S.M. Bajjalieh, D.T. Chiu (2011) “A quantitative single vesicle imaging technique reveals a subset of synaptic vesicle membrane proteins are trafficked with high precision” J. Neurosci. 31, 1461-1470.

C. Wu, S.J. Hansen, Q. Hou, J. Yu, M. Zeigler, Y. Jin, D.R. Burnham, J.D. McNeill, J.M. Olson, D.T. Chiu (2011) “Design of highly emissive polymer blend bioconjugates for in vivo tumor targeting” Angew. Chem. Int. Ed. 50, 3430-3434.

F. Ye, C. Wu, Y. Jin, Y.H. Chan, X. Zhang, D.T. Chiu (2011) “Ratiometric temperature sensing with semiconducting polymer dots” J. Amer. Chem. Soc. 133, 8146-8149.

P.G. Schiro, M. Zhao, J.S. Kuo, T. Schneider, K.M. Koehler, D.E. Sabath, D.T. Chiu (2012) “Sensitive and high-throughput isolation of rare cells from peripheral blood with ensemble decision aliquot ranking” Angew. Chem. Int. Ed. 51, 4618-4622.

C. Wu and D.T. Chiu (2013) “Semiconducting polymer dots: A new family of fluorescent nanoparticles for biological applications” Angew. Chem. Int. Ed. 52, 3086-3109.

T. Schneider, J. Kreutz, D.T. Chiu (2013) “The potential impact of droplet microfluidics in biology” Anal. Chem. 85, 3476-3482.


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