Lara Gamble

Research Associate Professor

lgamble@uw.edu
Phone: (206)616-4173
Office: MolES 226

UW Bioengineering faculty Lara Gamble

Lab Website
How I am inventing the future of medicine
My group is developing and applying new surface analysis methods to achieve a better understanding of biomedical problems.
Research Interests
Surface Analysis
Surface Modification
Biomaterials
Mass Spectral Imaging
Research Description
My research involves many different areas, but the main tie involves surface modification and characterization. One of my long-standing interests has been in development and analysis of biomaterials including fundamental research for the preparation and characterization of DNA and protein microarrays. By understanding how molecules interact with surfaces, it is possible to create well understood and better characterized microarrays.

My newer research involves the development of techniques for improved analysis of the biomolecule-surface interfaces (for example nanoparticles) as well as improved chemical imaging of biologically relevant samples. Imaging secondary ion mass spectrometry is a surface characterization technique that can provide molecular information with sub-micron resolution. Utilizing the newer cluster ion sources allows us to get 3-D images of cells and tissues as well. The ability to get sub-cellular images of cell and tissues with chemical and molecular specificity would open up a range of possibilities for better understanding and diagnosing biological processes such as tumor microenvironments, lipid metabolomics relationship to cancer, tissue repair, and chemical analysis of tissue and/or cells on a cellular and sub-cellular level. My research involves development of the sample preparation, technique development, data processing, and data analysis is critical to make this technology more applicable to the biological/medical community.

Education
PhD, Physical Chemistry, University of Washington
BA, University of California, Santa Cruz
Postdoc Information
Research Scientist, Zyomyx, Inc.
Senior Scientist, Space Dynamics Laboratory
Awards and Honors
UW Bioengineering Courses Taught
BIOEN 511: Biomaterials Seminar
Selected Publications
A. Harrison, H. Binder, A. Buhot, C. J. Burden, E. Carlon, C. Gibas, L. J. Gamble, A. Halperin, J. Hooyberhs, D. P. Kreil, R. Levicky, P. A. Novel, A. Ott, B. M. Pettitt, D. Tautz, A. E. Pozhitkov, “Physico-chemical Foundations underpinning microarray and next-generation sequencing experiments”, Nucleic Acids Research, 2013, 1-18. DOI:10.1093/nar/gks1358

Archana N. Rao, Nicolas Vandencasteele, Lara J. Gamble, and David W. Grainger, “High-Resolution Epifluorescence and Time-of-Flight Secondary Ion Mass Spectrometry Chemical Imaging Comparisons of Single DNA Microarray Spots”, Analytical Chemistry 2012 84 (24), 10628-10636. DOI: 10.1021/ac3019334

C.-Y. Lee, P. Gong, G.M. Harbers, D.W. Grainger, D.G. Castner, and L.J. Gamble, “Surface Coverage and Structure of Mixed DNA/Alkylthiol Monolayers on Gold: Characterization by XPS, NEXAFS, and Fluorescence Intensity Measurements”, Anal. Chem., 78, p3316-3325, 2006.

P. Gong, C.-Y. Lee, L.J. Gamble, D.G. Castner, and D.W. Grainger, “Hybridization Behavior of Mixed DNA/Alkylthiol Monolayers on Gold: Characterization by Surface Plasmon Resonance and 32P Radiometric Assay”, Anal. Chem., 78, p3326-3334, 2006

 

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