Project Description

Research Assistant Professor
Phone: (206)685-0452
Office: MolES G21

UW Bioengineering faculty Patrick Koelsch

I am inventing the future of medicine by developing novel experimental tools which allows the examination of biomolecules with molecular-level resolution in situ and in vitro.
Nonlinear Optics
Understanding chemical and structural properties of biomolecules is essential for the optimization and development process of biomedical devices. Biomolecules as well as functional organic molecules are often residing at interphases such as cellular membranes, biomaterials surfaces, DNA chips, switchable surfaces, nanoparticles, or microenvironment of cells – just to name a few. Common to those systems is that the species of interest are located at a buried interface, e.g. the interphase in between an artificial substrate and adherent cells or DNA immobilized on a solid surface adjacent to a solution etc. Our goal is to examine these systems with molecular-level resolution and in their natural occurring environment.

My group is utilizing optical techniques, in particular non-linear optical methods, that can be operated in liquids and have superior surface sensitivity and specificity. We are further developing and applying state-of-the-art spectroscopic and imaging techniques such as picosecond and femtosecond vibrational sum-frequency-generation spectroscopy in reflection and scattering modes as well as second-harmonic-generation imaging.

University of Heidelberg, Heidelberg, Germany, Venia Legendi, Physical Chemistry, 2010

Max-Planck-Institute for Colloid and Interface Science, Potsdam, Germany, Doctor Rerum Naturalium, Physics, 2005

University of Potsdam, Potsdam, Germany, Diplom, Physics, 2003

UK Science and Innovation Collaboration Development Award, 2012, UK Department for Business Innovation and Skills.
Innovations fond FRONTIER, 2009, University of Heidelberg.
Temporary Position for Principle Investigator, 2007, German Science Foundation.
Travel Award, 2008, German Science Foundation.
Prize for the best Ph.D. thesis in the field of colloid- and interface science, 2006, Max-Planck-Society.
BIOEN 511: Biomaterials Seminar
Koelsch, P.; Muglali, M. I.; Rohwerder, M.; Erbe, A., Third-order effects in resonant sum-frequency-generation signals at electrified metal/liquid interfaces. J. Opt. Soc. Am. B 2013, 30, (1), 219-223.

Pop-Georgievski, O.; Verreault, D.; Diesner, M.-O.; Proks, V.; Heissler, S.; Rypáček, F.; Koelsch, P., Nonfouling Poly(ethylene oxide) Layers End-Tethered to Polydopamine. Langmuir 2012, 28, (40), 14273-14283.

Barth, C.; Jakubczyk, D.; Kubas, A.; Anastassacos, F.; Brenner-Weiss, G.; Fink, K.; Schepers, U.; Bräse, S.; Koelsch, P., Interkingdom Signaling: Integration, Conformation, and Orientation of N-Acyl-l-homoserine Lactones in Supported Lipid Bilayers. Langmuir 2012, 28, (22), 8456-8462.

Diesner, M.-O.; Welle, A.; Kazanci, M.; Kaiser, P.; Spatz, J.; Koelsch, P., In vitro observation of dynamic ordering processes in the extracellular matrix of living, adherent cells. Biointerphases 2011, 6, (4), 171-179.

Verreault, D.; Kurz, V.; Howell, C.; Koelsch, P., Sample cells for probing solid/liquid interfaces with broadband sum-frequency-generation spectroscopy. Rev. Sci. Instrum. 2010, 81, (6), 063111.

Kurz, V.; Grunze, M.; Koelsch, P., In Situ Characterization of Thermo-Responsive Poly(N-Isopropylacrylamide) Films with Sum-Frequency Generation Spectroscopy. ChemPhysChem 2010, 11, (7), 1425-1429.

Diesner, M. O.; Howell, C.; Kurz, V.; Verreault, D.; Koelsch, P., In Vitro Characterization of Surface Properties Through Living Cells. J. Phys. Chem. Lett. 2010, 1, (15), 2339-2342.

Howell, C.; Schmidt, R.; Kurz, V.; Koelsch, P., Sum-frequency-generation spectroscopy of DNA films in air and aqueous environments. Biointerphases 2008, 3, (3), Fc47-Fc51.

Howell, C.; Diesner, M. O.; Grunze, M.; Koelsch, P., Probing the Extracellular Matrix with Sum-Frequency-Generation Spectroscopy. Langmuir 2008, 24, (24), 13819-13821.

Koelsch, P.; Viswanath, P.; Motschmann, H.; Shapovalov, V. L.; Brezesinski, G.; Mohwald, H.; Horinek, D.; Netz, R. R.; Giewekemeyer, K.; Alditt, T. S.; Schollmeyer, H.; von Klitzing, R.; Daillant, J.; Guenoun, P., Specific ion effects in physicochemical and biological systems: Simulations, theory and experiments. Colloids Surf., A 2007, 303, (1-2), 110-136.

Koelsch, P.; Motschmann, H., An experimental route to Hofmeister. Curr. Opin. Colloid Interface Sci. 2004, 9, (1-2), 87-91.


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