Office: Foege N233B
My laboratory is investigating ways of ameliorating DNA damage and apoptosis in somatic and germ cells in order to slow the aging process and prevent diseases and we are using novel techniques to visualize cells with DNA damage and cells undergoing apoptosis in the wrong place at the wrong time.
We are also investigating compounds such as artemisinin (and its derivatives and novel conjugates and dimers) for killing cancer cells.
Sperm DNA integrity and fetal origins of adult disease
The ultimate goal of my research is the elucidation of the mechanisms responsible for aging and disease processes and application of these findings to ameliorate or prevent aging and age-related diseases, including cancer.
MBBS (KGMC, Lucknow University, India, 1972)
MS (KGMC, Lucknow University, India, 1976)
Post-doctoral Fellowship, DNA damage/repair, Ohio State University, 1979-1981
Special Issue of Mutation Research celebrating the 20th anniversary of the publication of the paper on Comet Assay (Singh N.P., McCoy M., Tice R.R. and Schneider E. (1988) A simple technique for quantitation of low levels of DNA damage in single cells. Experimental Cell Research 175: 184-191)
Fellow of the Japanese Society for Promotion of Science
Editorial board: Mutation Research, Biogerontology
Colgate-Palmolive Visiting Professor Award, Yale University.
Bioengineering 499: Special Projects 2008-present
Bioengineering 598: Research Rotation 2012-present
Bioengineering 600: Independent Study/Research 2013-present
Bioengineering Senior Capstone Project Mentorships 2013-present
Singh N.P., McCoy M., Tice R.R. and Schneider E. (1988) A simple technique for quantitation of low levels of DNA damage in single cells. Experimental Cell Research 175: 184-191.
Singh N.P., Danner D.B., Tice R.R., Brant L. and Schneider E.L. (1990) DNA damage and repair with age in individual human lymphocytes. Mutation Research 237: 123-130.
Singh N.P., Danner D.B., Tice R.R., Pearson J.D., Brant L.J., Morrell C.H. and Schneider E.L. (1991) Basal DNA damage in human lymphocytes with age. Mutation Research 256: 1-6.
Lai H and Singh NP (1995). Selective cancer cell cytotoxicity from exposure to dihydroartemisinin and holotransferrin. Cancer Letters, 91: 41-46.
Singh NP. Stephens RE. X-ray induced DNA double-strand breaks in human sperm. Mutagenesis, 13:75–9, 1998.
Singh NP, Stephens RE, Singh H and Lai H. Visual quantification of DNA double-strand breaks in bacteria. Mutation Research, 429:159–168, 1999.
Singh NP. A simple method for accurate estimation of apoptotic cells. Experimental Cell Research, 256: 328–337, 2000.
Singh NP and Lai H (2001). Selective toxicity of dihydroartemisinin and holotransferrin toward human breast cancer cells. Life Sciences 70:49-56.
Singh NP, Ogburn CE, Wolf NS, van Belle G and Martin MG. DNA double-strand breaks in mouse kidney cells with age. Biogerontology, 2:261–270, 2001.
Duty SM, Singh NP, Ryan L, Chen Z, Lewis C, Huang T and Hauser R. Reliability of the comet assay in cryopreserved human sperm. Human Reproduction, 17:1274–1280, 2002.
Singh NP, Penn PE, Pendergrass WR and Wolf NS. White light-mediated DNA strand breaks in lens epithelial cells. Experimental Eye Research, 75:555–560, 2002.
Duty SM, Singh NP, Silva MJ, Barr DB, Brock JW, Ryan L, Herrick RF, Christiani DC and Hauser R. The relationship between environmental exposure to phthalates and DNA damage in human sperm using the neutral comet assay. Environmental Health Perspectives, 111:1164–9, 2003.
Singh NP, Muller CH and Berger RE. Effects of age on DNA double strand breaks and apoptosis in human sperm. Fertility and Sterility, 18:1420–1430, 2003.
Singh NP and Lai H (2004). Artemisinin Induces Apoptosis in Human Cancer Cells. AntiCancer Research 24: 2277-2280.
Lai H, Sasaki T, Singh NP and Messay A (2005). Effects of artemisinin-tagged holotransferrin on cancer cells. Life Sciences 76:1267-1274
Singh NP and Lai H (2005). Synergistic Cytotoxicity of Artemisinin and Sodium Butyrate on Human Cancer Cells. Anticancer Research 25: 4325-
Lai H and Singh NP (2006). Oral artemisinin prevents and delays the development of 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancer in the rat. Cancer Letters 231:43-48.
Singh NP and Panwar VK (2006). Case Report of a Pituitary Macroadenoma Treated With Artemether. Integrative Cancer Therapies 5: 391-394.
Toraason M., Lynch D.W., DeBord D.G., Singh N., Krieg E., Butler M.A., Toennis C.A. and Nemhauser J.B. (2006) DNA damage in leukocytes of workers occupationally exposed to 1-bromopropane. Mutation Research 603: 1-14.
Oh S, Kim BJ, Singh NP, Lai H, and Sasaki T (2009). Synthesis and anti-cancer activity of covalent conjugates of artemisinin and a transferrin-receptor targeting peptide. Cancer Letters 274:33-39.
Nakase I, Gallis B, Takatani-Nakase T, Oh S, Lacoste E, Singh NP, Goodlet DR, Tanaka S, Futaki S, Lai H, and Sasaki T (2009). Transferrin receptor-dependent cytotoxicity of artemisinin-transferrin conjugates on prostate cancer cells and induction of apoptosis. Cancer Letters 274:290-298.
Singh NP, Ferreira, JFS, Park, JS and Lai, HC (2011). Cytotoxicity of Ethanolic Extracts of Artemisia annua to Molt-4 Human Leukemia Cells. Planta Medica 77: 1788-1793.
Singh NP, Lai HC, Park JS, Gerhardt TE, Kim BJ, Wang, S and Sasaki, T (2011) Effects of Artemisinin Dimers on Rat Breast Cancer Cells in vitro and in vivo. AntiCancer Research 31:4111-4114.
Krieg EF, Mathias PI, Toennis CA, Clark JC, Marlow KL, B’Hymer C, Singh NP, Egeghy P, Gibson RL, Butler MA (2012) Detection of DNA damage in workers exposed to JP-8 jet fuel. Mutation Research 747:218-227.
Lai HC, Singh, NP and Sasaki T. (2013) Development of artemisinin compounds for cancer treatment. Investigational New Drugs 31:230-46.
Chan HW, Singh NP and Lai HC (2013) Cytotoxicity of Dihydroartemisinin Toward Molt-4 Cells Attenuated by N-Tert-butyl-alpha-phenylnitrone and Deferoxamine. Anticancer Research 33: 4389-4394.
Park J, Lai HC, Singh M, Sasaki T and Singh NP (2014) Development of a Dihydroartemisinin-resistant Molt-4 leukemia cell line. Anticancer Research 34: 2807-2810.
Wickerath and Singh (2014) Additive Cytotoxic Effects of Dihydroartemisinin and Sodium Salicylate on Cancer Cells Anticancer Research 34: 3399-401