Paul Yager

Professor

yagerp@uw.edu
Phone: (206)543-8063
Office: Foege N530J

UW Bioengineering faculty Paul Yager

Lab Website

How I am inventing the future of medicine

Our lab focuses on development of a new class of low cost point-of-care diagnostic technologies utilizing microfluidics, particularly paper-based systems imaged by cell phones, with a long range goal of increasing access to healthcare in the developed an developing worlds. We are hoping to help to commercialize these technologies in the near future with partners outside of UW.
Research Interests

Microfluidics
Immunoassays
Nucleic acid assays
Paper
2DPN
Microfluidics 2.0
Point-of-care diagnostics
Global health
Translational medicine.
Research Description

The two major projects in the lab at this time focus on the use of what we call two-dimension paper networks (2DPNs):
1) Development of paper-based immunoassays for pathogens like the influenza virus that have enhanced sensitivity
2) Development of the first instrument-free point-of-care technology for sample-to-result detection of pathogens using isothermal nucleic acid amplification.
Both projects involve collaborations with multiple organizations, including industry. Work in the lab includes physical biochemistry, immunoassay development, materials science, development of optical methods for sensitive chemical detection, nucleic acid amplification, microfabrication, cell-phone imaging, and computational fluid dynamics.
Education

Ph.D., University of Oregon, Chemistry, 1980
AB, Princeton University, Biochemistry, 1975
Postdoc Information

1980-82 NRC Resident Research Associate, Naval Research Laboratory
Awards and Honors

1999 Selected as a member of the College of Fellows, American Institute for Medical and Biological Engineering (AIMBE)
1988 Nominated by UW for Packard Fellowship
1985 Nominated for National Science Foundation Waterman Award
UW Bioengineering Courses Taught

BIOEN 573 Biosensors, Principles and Practice
BIOEN 401 Capstone Design BioE 401
BIOEN 599C and D Microfabrication for Bioengineering Applications,
BIOEN 578 Biomembranes
Selected Publications

Disposable hydrogen fuel cells for powering next-generation lateral flow, Esquivel, J.P., Buser, J.R., del Campo, F.J., Rojas, S., Yager, P. and Sabaté, N., Transducers 15, in press

One-step purification and concentration of DNA in porous membranes for point-of-care applications, Byrnes, S.A., Bishop, J.D., Lafleur, L.K., Buser, J.R., Lutz, B. and Yager, P., Lab on a Chip, 15: 2647-2659 (2015)

Enabling the development and deployment of next generation point-of-care diagnostics, Derda, R., Gitaka, J., Klapperich, C.M., Mace, C.R., Kumar, A.A., Lieberman, M., Linnes, J.C., Jores, J., Nasimolo, J., Ndung’u, J., Taracha, E., Weaver, A., Weibel, D.B., Kariuki, T.M. and Yager, P., PLoS Neglected Tropical Diseases, 9(5): e0003676. doi:10.1371/journal.pntd.0003676 (2015)

Electromechanical cell lysis using a portable audio device: enabling challenging sample preparation at the point-of-care, Buser, J.F., Wollen, A., Heiniger, E.K., Byrnes, S., Kauffman, P.C., Ladd, P.D. and Yager, P.,  Lab on a Chip, 15(9): 1994-1997 (2015)

A versatile valving toolkit for automating fluidic operations in paper microfluidic devices, Toley, B.J., Wang, J.A., Gupta, M., Buser, J.R., Lafleur, L.K., Lutz, B.R., Fu, E. and Yager, P.,  Lab on a Chip, 15(6): 1432-1444 (2015)

Microfluidic Diagnostics for Low-Resource Settings: Improving Global Health without a Power Cord, Buser, J.R., Holstein, C.A., and Yager, P., in Microfluidics for Medical Applications, Albert van den Berg and Loes Segerink, eds., RSC Press, 151-190 (2015)

Highly sensitive immunoassay based on controlled rehydration of patterned reagents in a 2-dimensional paper network, Fridley, G., Le, H. and Yager, P., Analytical Chemistry, 86(13): 6447-6453 (2014)

Long-term dry storage of an enzyme-based reagent system for ELISA in point-of-care devices, Ramachandran. S., Fu, E., Lutz, B. and Yager. P., Analyst, 139; 1456-1462 (2014)

Tunable-delay shunts for paper microfluidic devices, Toley, B.J., McKenzie, B., Liang, T., Buser, J., Yager, P. and Fu, E., Analytical Chemistry. 85(23): 11545-11552 (2013)

Dissolvable fluidic time delays for programming multi-step assays in instrument-free paper diagnostics, Lutz, B., Liang, T., Fu, E., Ramachandran, S., Kauffman, P. and Yager, P., Lab on a Chip, 13(14): 2840-2847 (2013)

 

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