Project Description

Professor

yagerp@uw.edu
Phone: (206)543-8063
Office: Foege N530J

UW Bioengineering faculty Paul Yager

Our lab focuses on development of a new class of low cost point-of-care diagnostic technologies.  These utilize microfluidics, particularly paper-based systems imaged by cell phones.  Our long range goal is increasing access to healthcare in the developed and developing worlds. We are hoping see these technologies commercialized in the near future with partners inside and outside UW.

Microfluidics
Immunoassays
Nucleic acid assays
Paper
2DPN
Microfluidics 2.0
Uses of smart phones for bioassays
Point-of-care diagnostics
Decentralization of medical testing
Global health

The two major lines of research in the lab at this time focus on the use of what we call two-dimension paper networks (2DPNs):
1) Development of new high-sensitivity paper-based protein-binding assays for pathogens like the influenza and Ebola viruses
2) Development of the first instrument-free point-of-care technology for sample-to-result detection of pathogens using isothermal nucleic acid amplification.
Both projects involve collaborations with multiple organizations, including nonprofits and industry. Work in the lab includes physical biochemistry, immunoassay development, materials science, development of optical methods for sensitive chemical detection, nucleic acid amplification, microfabrication, cell-phone imaging, and computational fluid dynamics. Every effort is made to see to it that the technology being developed is compatibly with the technical, social and economic constraints the apply for use in low-resource communities.

Ph.D., University of Oregon, Chemistry, 1980
AB, Princeton University, Biochemistry, 1975
1980-82 NRC Resident Research Associate, Naval Research Laboratory

2015 UW College of Engineering Faculty Award: Research
2014 Seattle Business Magazine’s Leaders in Health Care Award
2013 Elected to Washington State Academy of Sciences
2012 Seattle Magazine’s Top Doctors 2012: Global Health Award, 2012
1999 Selected as a member of the College of Fellows, American Institute for Medical and Biological Engineering (AIMBE)

BIOEN 573 Biosensors, Principles and Practice
BIOEN 401 Capstone Design BioE 401
BIOEN 599C and D Microfabrication for Bioengineering Applications,
BIOEN 578 Biomembranes
Disposable hydrogen fuel cells for powering next-generation lateral flow, Esquivel, J.P., Buser, J.R., del Campo, F.J., Rojas, S., Yager, P. and Sabaté, N., Transducers 15, in press

One-step purification and concentration of DNA in porous membranes for point-of-care applications, Byrnes, S.A., Bishop, J.D., Lafleur, L.K., Buser, J.R., Lutz, B. and Yager, P., Lab on a Chip, 15: 2647-2659 (2015)

Enabling the development and deployment of next generation point-of-care diagnostics, Derda, R., Gitaka, J., Klapperich, C.M., Mace, C.R., Kumar, A.A., Lieberman, M., Linnes, J.C., Jores, J., Nasimolo, J., Ndung’u, J., Taracha, E., Weaver, A., Weibel, D.B., Kariuki, T.M. and Yager, P., PLoS Neglected Tropical Diseases, 9(5): e0003676. doi:10.1371/journal.pntd.0003676 (2015)

romechanical cell lysis using a portable audio device: enabling challenging sample preparation at the point-of-care, Buser, J.F., Wollen, A., Heiniger, E.K., Byrnes, S., Kauffman, P.C., Ladd, P.D. and Yager, P., Lab on a Chip, 15(9): 1994-1997 (2015)

A versatile valving toolkit for automating fluidic operations in paper microfluidic devices, Toley, B.J., Wang, J.A., Gupta, M., Buser, J.R., Lafleur, L.K., Lutz, B.R., Fu, E. and Yager, P., Lab on a Chip, 15(6): 1432-1444 (2015)

Microfluidic Diagnostics for Low-Resource Settings: Improving Global Health without a Power Cord, Buser, J.R., Holstein, C.A., and Yager, P., in Microfluidics for Medical Applications, Albert van den Berg and Loes Segerink, eds., RSC Press, 151-190 (2015)

Highly sensitive immunoassay based on controlled rehydration of patterned reagents in a 2-dimensional paper network, Fridley, G., Le, H. and Yager, P., Analytical Chemistry, 86(13): 6447-6453 (2014)

Long-term dry storage of an enzyme-based reagent system for ELISA in point-of-care devices, Ramachandran. S., Fu, E., Lutz, B. and Yager. P., Analyst, 139; 1456-1462 (2014)

Tunable-delay shunts for paper microfluidic devices, Toley, B.J., McKenzie, B., Liang, T., Buser, J., Yager, P. and Fu, E., Analytical Chemistry. 85(23): 11545-11552 (2013)

Dissolvable fluidic time delays for programming multi-step assays in instrument-free paper diagnostics, Lutz, B., Liang, T., Fu, E., Ramachandran, S., Kauffman, P. and Yager, P., Lab on a Chip, 13(14): 2840-2847 (2013)

 

In the News

  • paul-yager-coe-600-300

Paul Yager receives over $4M to continue developing paper-based diagnostic device, amongst top Q2 2015 UW Medicine research award recipients

August 11th, 2015|Comments Off on Paul Yager receives over $4M to continue developing paper-based diagnostic device, amongst top Q2 2015 UW Medicine research award recipients

  • busy-lab

Paul Yager, Leslie Chan and Christopher Adams receive 2015 College of Engineering Awards

April 30th, 2015|Comments Off on Paul Yager, Leslie Chan and Christopher Adams receive 2015 College of Engineering Awards

  • Krittika D'Silva in India July 2014

Student’s smartphone apps connect remote populations to health care, help

March 18th, 2015|Comments Off on Student’s smartphone apps connect remote populations to health care, help

  • paul-yager-TEDX

Paul Yager presents at TEDXRainier Nov. 22, 2014 (updated video)

November 25th, 2014|Comments Off on Paul Yager presents at TEDXRainier Nov. 22, 2014 (updated video)

  • Yager at-home test with cell phone

Making good: Desire to help others inspires diverse research

December 15th, 2014|Comments Off on Making good: Desire to help others inspires diverse research

Programming in Paper

December 15th, 2014|Comments Off on Programming in Paper