Course: BIOEN 457: Advanced Molecular Bioengineering
Instructor: Patrick Stayton
Texts and Supplemental Materials: No single textbook required, uses Chapters from “Enzyme Structure and Mechanism” by Fersht, “Physical Chemistry” by Alberty, and from different reaction kinetics textbooks.
UW Catalog Description: Fundamentals of molecular recognition: thermodynamics, forces, kinetics. Manipulation of recognition processes for current molecular bioengineering research and development. Fundamental physical chemistry of molecular recognition in the context of biomedicine. Therapeutics based on cells.
Prerequisites by Course: BIOEN 315 and BIOEN 335
Prerequisites by Topic: Molecular bioengineering, general biochemistry
Required or Elective: Elective
Specific Outcomes: By the end of this course, students will be able to:
- Define thermodynamic principles in context of biomolecular recognition and stability
- Define kinetic theory and reaction kinetics in context of biomolecular recognition and stability
- Define how non-covalent bonding energetics relate to biomolecular recognition and stability
- Apply biomolecular energetics to biotherapeutic design from a modeling and computational standpoint
- Apply combinatorial and directed evolution approaches to biotherapeutic design
- Evaluate engineering systems analysis to receptor trafficking in context of how biological feedback systems affect therapeutic strategies
- Define molecular design strategies for modifying cells to develop cellular therapeutics
Outcomes Addressed by this Course:
A. An ability to apply knowledge of mathematics, science, and engineering.
C. An ability to design a system, component, r process to meet desired needs within realistic constraints.
G. An ability to communicate effectively.
- Molecular Recognition Fundamentals 1: thermodynamics of biomolecular interactions, non-covalent forces underlying bioenergetics: hydrogen bonding, van der Waals, hydrophobic effect, water in context of molecular recognition biomolecular stability
- Molecular Recognition Fundamentals 2: kinetic theory, reaction kinetics, enzyme energetics
- Rational Biotherapeutic Design: molecular modeling, computational approaches to predicting energetics
- Directed Evolution for Biotherapeutic Design: random mutagenesis approaches and techniques, phage display and selection techniques, combinatorial approaches and techniques
- Cellular Warfare: receptor-mediated recognition in immune system surveillance, macrophage-B-Cell collaboration, T-Cell and natural killer cell function, vaccines