Department of Biochemistry Box 357350 University of Washington Seattle, WA 98195
 



 
 
 
         
Guenter Daum                     

Adjunct Research Associate Professor of Biochemistry
Research Associate Professor of Surgery

206.897.1551. V
206.897.1553 F
daum@u.washington.edu

 

Research

We are interested in the regulation of signaling pathways that are activated in smooth muscle cells (SMCs) after vessel injury (e.g. after angioplasty) or in atherosclerosis. In the normal vessel wall, SMCs are quiescent because of the lack of activatory stimuli and the presence of growth inhibitory factors (e.g. nitric oxide). After injury, or in diseased vessels, SMCs are exposed to a different environment that may induce proliferation, migration, or programmed cell death (apoptosis). Modulation of SMC function in these circumstances may be of therapeutic value. We are particularly interested in the role of oxidative stress in SMC activation.


Selected Publications

Chen L, Daum G, Chitaley K, Coats SA, Bowen-Pope DF, Eigenthaler M, Thumati NR, Walter U, Clowes AW (2004) Vasodilator-stimulated phosphoprotein regulates proliferation and growth inhibition by nitric oxide in vascular smooth muscle cells. Arterioscler Thromb Vasc Biol 24, 1403-1408.

Millette E, Rauch BH, Defawe O, Kenagy RD, Daum G, Clowes AW (2005) Platelet-derived growth factor-BB-induced human smooth muscle cell proliferation depends on basic FGF release and FGFR-1 activation. Circ Res 96, 172-179.

Rauch BH, Millette E, Kenagy RD, Daum G, Fischer JW, Clowes AW (2005) Syndecan-4 is required for thrombin-induced migration and proliferation in human vascular smooth muscle cells. J Biol Chem 280, 17507-17511.

Chitaley K, Chen L, Galler A, Walter U, Daum G, Clowes AW (2004) Vasodilator-stimulated phosphoprotein is a substrate for protein kinase C. FEBS Lett 556, 211-215.

Englesbe MJ, Davies MG, Hawkins SM, Hsieh PC, Daum G, Kenagy RD, Clowes AW (2004) Arterial injury repair in nonhuman primates-the role of PDGF receptor-beta. J Surg Res 119, 80-84.

Englesbe MJ, Deou J, Bourns BD, Clowes AW, Daum G (2004) Interleukin-1beta inhibits PDGF-BB-induced migration by cooperating with PDGF-BB to induce cyclooxygenase-2 expression in baboon aortic smooth muscle cells. J Vasc Surg 39, 1091-1096.

Englesbe MJ, Hawkins SM, Hsieh PC, Daum G, Kenagy RD, Clowes AW (2004) Concomitant blockade of platelet-derived growth factor receptors alpha and beta induces intimal atrophy in baboon PTFE grafts. J Vasc Surg 39, 440-446.

Rauch BH, Millette E, Kenagy RD, Daum G, Clowes AW (2004) Thrombin- and factor Xa-induced DNA synthesis is mediated by transactivation of fibroblast growth factor receptor-1 in human vascular smooth muscle cells. Circ Res 94, 340-345.

Chan AK, Kalmes A, Hawkins S, Daum G, Clowes AW (2003) Blockade of the epidermal growth factor receptor decreases intimal hyperplasia in balloon-injured rat carotid artery. J Vasc Surg 37, 644-649.

Nathe TJ, Deou J, Walsh B, Bourns B, Clowes AW, Daum G (2002) Interleukin-1beta inhibits expression of p21(WAF1/CIP1) and p27(KIP1) and enhances proliferation in response to platelet-derived growth factor-BB in smooth muscle cells. Arterioscler Thromb Vasc Biol 22, 1293-1298.

Kalmes A, Daum G, Clowes AW (2001) EGFR transactivation in the regulation of SMC function. Ann N Y Acad Sci 947, 42-54; discussion 54-45.

Chen L, Daum G, Fischer JW, Hawkins S, Bochaton-Piallat ML, Gabbiani G, Clowes AW (2000) Loss of expression of the beta subunit of soluble guanylyl cyclase prevents nitric oxide-mediated inhibition of DNA synthesis in smooth muscle cells of old rats. Circ Res 86, 520-525.

Kalmes A, Vesti BR, Daum G, Abraham JA, Clowes AW (2000) Heparin blockade of thrombin-induced smooth muscle cell migration involves inhibition of epidermal growth factor (EGF) receptor transactivation by heparin-binding EGF-like growth factor. Circ Res 87, 92-98.

Kalmes A, Deou J, Clowes AW, Daum G (1999) Raf-1 is activated by the p38 mitogen-activated protein kinase inhibitor, SB203580. FEBS Lett 444, 71-74.