Research

We are interested in the structure and function of the spindle pole body in the budding yeast, Saccharomyces cerevisiae. Our focus is on the requirements for microtubule nucleation. We are also engaged in worldwide collaborations, funded by the NIH National Center for Research Resources (NCRR), to map the arrangement of protein components within large macromolecular complexes using microscopy-based FRET (fluorescence resonance energy transfer). As part of this effort we have developed a new FRET standard and FRET analysis software. See the Yeast Resource Center (YRC) website for additional information.

Selected publications

Kollman, J.M., Zelter, A., Muller, E.G., Fox, B., Rice, L.M., Davis, T.N., and Agard, D.A. (2007). The Structure of the {gamma}-Tubulin Small Complex: Implications of Its Architecture and Flexibility for Microtubule Nucleation. Mol Biol Cell.

Mc Intyre, J., Muller, E.G., Weitzer, S., Snydsman, B.E., Davis, T.N., and Uhlmann, F. (2007). In vivo analysis of cohesin architecture using FRET in the budding yeast Saccharomyces cerevisiae. Embo J 26, 3783-3793.

Shimogawa, M.M., Graczyk, B., Gardner, M.K., Francis, S.E., White, E.A., Ess, M., Molk, J.N., Ruse, C., Niessen, S., Yates, J.R., 3rd, Muller, E.G., Bloom, K., Odde, D.J., and Davis, T.N. (2006). Mps1 phosphorylation of Dam1 couples kinetochores to microtubule plus ends at metaphase. Curr Biol 16, 1489-1501.

Yoder, T.J., McElwain, M.A., Francis, S.E., Bagley, J., Muller, E.G., Pak, B., O'Toole, E.T., Winey, M., and Davis, T.N. (2005). Analysis of a spindle pole body mutant reveals a defect in biorientation and illuminates spindle forces. Mol Biol Cell 16, 141-152.

Muller, E.G., Snydsman, B.E., Novik, I., Hailey, D.W., Gestaut, D.R., Niemann, C.A., O'Toole, E.T., Giddings, T.H., Jr., Sundin, B.A., and Davis, T.N. (2005). The organization of the core proteins of the yeast spindle pole body. Mol Biol Cell 16, 3341-3352.

Sundin, B.A., Chiu, C.H., Riffle, M., Davis, T.N., and Muller, E.G. (2004). Localization of proteins that are coordinately expressed with Cln2 during the cell cycle. Yeast 21, 793-800.

Flory, M.R., Carson, A.R., Muller, E.G., and Aebersold, R. (2004). An SMC-domain protein in fission yeast links telomeres to the meiotic centrosome.

Muller, E.G. (1996). A glutathione reductase mutant of yeast accumulates high levels of oxidized glutathione and requires thioredoxin for growth. Mol Biol Cell 7, 1805-1813.

Muller, E.G. (1991). Thioredoxin deficiency in yeast prolongs S phase and shortens the G1 interval of the cell cycle. J Biol Chem 266, 9194-9202.