Image Gallery

John Aitchison
Dynamics of biological responses are controlled through networks of interacting components. This concept lies at the heart of systems biology. The Aitchison laboratory exploits yeast to develop systems approaches to interrogate, model and understand complex biological processes, and applies the advances of systems biology to infectious diseases critical to global health.

Niels Andersen
Exploring Protein Folding Landscapes by Circular Permutation

Chip Asbury
The composition and movement of individual protein complexes can be measured simultaneously using total internal reflection fluorescence (TIRF) microscopy. Here, single Dam1 complexes (green) bind and move along a microtubule filament (red) without detaching. This observation shows that Dam1 can contribute to chromosome-microtubule coupling during cell division without necessarily forming a sixteen-membered ring encircling the filament.

 

David Baker
The crystal structure of a de novo designed protein is nearly identical to the in silico model.

Jesse Bloom
Understanding the molecular evolution of proteins and viruses.

 

Steve Carlson
Surface of the nerve terminal at the electric organ synapse. The red is the alpha subunit of the voltage-gated calcium channel. The green is the synaptic vesicle protein SV2 on the surface of the nerve terminal awaiting endocytosis.

Carlos Catalano
Assembly of an Infectious Virus. The terminase enzyme assembles at a cos site in a multi-genome concatemer. This maturation complex nicks the duplex and then binds to the portal ring of a procapsid shell to afford the packing motor complex. The motor packages DNA into the capsid to liquid-crystalline density generating over 30 atmospheres of pressure. Addition of finishing proteins and the phage tail complete the infectious virus.

 

Champak Chatterjee
Investigating the effect of histone modifications on chromatin
structure and function

Dan Chiu
Two-color fluorescence (top panels) and atomic force microscopy (bottom panels) images of individual synaptic vesicles

 

Valerie Daggett

Adrienne Fairhall

 

Mike Gelb
The enzyme phospholipase A2 sitting on the membrane interface. This is an interfacial enzyme that must bind to the phospholipid bilayer to access its water-insoluble phospholipid substrate. The x-ray structure of the enzyme was used together with a novel EPR method developed in the Gelb and Robinson labs to position the enzyme on the membrane surface.

Sharona Gordon
TRPV1 channels diffusing laterally through plasma membrane of F11 cells.

 

Steven Hahn
Structure for one form of the complex between the transcription activator Gcn4 and the coactivator Gal11 - figure credit Steven Hahn and Rachel Klevit

Wim Hol
Type II secretion system from Vibrio cholera, which translocates cholera toxin (yellow in the center) across the outer membrane.

 

Sarah Keller
Figure shows two-color fluorescence (top panels) and atomic force microscopy (bottom panels) images of individual synaptic vesicles; here, we developed a quantitative microscopy technique for counting the number of membrane proteins on synaptic vesicles.

Patrick Koelsch
Osteoclast Bones 36A 4x zoom z-stack 3D view

 

Kelly Lee
Cryo-electron tomography reveals influenza virus architecture including the virus' hemagglutinin fusion protein spikes on the surface. This technique is being used to understand how the viral membrane and a target host membrane fuse together during cell invasion.

Lutz Maibaum
Grand-canonical computer simulation of two actin filament bundles polymerizing into the cell membrane.

 

Dustin Maly
Protein kinases are dynamic enzymes that can exist in several different conformations. This image shows a fluorescently labeled probe binding to SRC kinase in the DFG-out conformation. This binding event causes an increase in fluorescence, which allows the thermodynamics and kinetics of this interaction to be determined.

Ethan Merritt
Key proteins in eukaryotic pathogens can be intriguingly
different from their human homologs, making them suitable
targets for structure-based drug design.

 

Alex Merz
Membrane tethering and fusion systems in eukaryotic cells. A range of biochemical and biophysical approaches are applied to understand how small G proteins and multisubunit tethering complexes control the assembly of membrane fusion machines.

Mike Regnier
Cardiac muscle sarcomere thin and thick filaments

 

Fred Rieke
Midget and parasol ganglion cells, two of the primary outputs of the retina.

Julian Simon
Embryonic zebrafish neuromasts labeled with fluorescent dyes serve as a useful screening model system for identification of compounds that protect mammalian auditory hair cells against drug-induced toxicity.

 

Pat Stayton
Smart Polymer-Enzyme Conjugates. Stimuli-responsive polymers are conjugated to engineered sites near the enzyme active site and used to switch enzyme activity via pH or temperature controlled structural transitions.

Ron Stenkamp
Diffraction pattern from x-ray crystallography

 

Barry Stoddard
Crystal structure of the I-AniI LAGLIDADG homing endonuclease. This protein and others like it are used for gene targeting in a number of biotechnology and medical applications

Roland Strong
Siderocalin is an antibacterial, innate immune system defense protein that functions by sequestering essential iron away from invading pathogens as complexes with bacterial siderophores - discovered completely serendipitously when recombinant protein co-crystallized with ferric enterochelin, the primary siderophore of E. coli.

 

Wendy Thomas
The bacterial adhesive protein FimH is a mechanical force sensor that switches to a tight-binding state when stretched

Gabriele Varani
By using ultrafast NMR methods, it is possible to observe changes in RNA spectra in real time with resolution of just a few seconds and establish the conformational pathway by which RNA changes structure in atomic detail.

 

joshuah vaughan

Joshua Vaughan
(read the paperĀ here)

Christophe Verlinde
Model of T. brucei MetRS with potent inhibitor docked in the active site.

 

Liguo Wang
Micrograph of BK proteoliposomes tethered to a streptavidin crystal

Paul Wiggins

 

Linda Wordeman
TIRF image of a GFP-labeled kinesins implicating in modulating the dynamics of microtubule ends (Motors: green; Microtubules: red).

Wenqing Xu

 

William Zagotta
Image of fluorescent ion channels in the membrane of a patch-clamp electrode. These recordings allow us to measure ion channel function with electrophysiology and structural rearrangements with fluorescence measurements simultaneously.

Ning Zheng
Cardiac troponin C + troponin I switch peptide

   

 

   

© UW Biological Physics, Structure and Design 2013