Effects of Charge State on the Structures of Serum Albumin Ions in the Gas Phase: Insights from Cation-to-Anion Proton-Transfer Reactions, Ion Mobility, and Mass Spectrometry. Meagan M. Gadzuk-Shea, Matthew F. Bush. J. Phys. Chem. B 2018, 122, in press. (Link)ABSTRACT
On August 1st, Rae Eaton, a graduate student in the Bush Lab, presented a talk entitled “Out of the Attic, Into the Lab: building a shrink ray is harder than it looks” The talk was part of the Science and a Movie program, a series put on by Central Cinema and the Pacific Science Center. The series pairs talks from scientists in the Seattle area with silly or classic movies.
In the talk, Rae used stories from the world of bioanalytical chemistry to discuss the challenges home inventors can run into working in unusual spaces, and explores the benefits a lab offers versus working by oneself in their garage. The talk was followed by a screening of “Honey I Shrunk the Kids”.
Thanks to Central Cinema and the Pacific Science Center’s Meet a Scientist program for the opportunity!
The Bush Lab was just awarded a grant from the National Science Foundation for a project titled “Bridging the Gap Between Observables from Ion Mobility Mass Spectrometry and the Structures of Native Proteins” (1807382). We are excited to pursue this research and are grateful for this financial support from the NSF.
Ion Mobility of Proteins in Nitrogen Gas: Effects of Charge State, Charge Distribution, and Structure. Daniele Canzani, Kenneth J. Laszlo, Matthew F. Bush. J. Phys. Chem. A 2018, in press. (Link)
Ion mobility is emerging as a rapid and sensitive tool for structural characterization. Collision cross-section (Ω) values determined using ion mobility are often compared to values calculated for candidate structures generated through molecular modeling. Several methods exist for calculating Ω values, but the trajectory method explicitly includes contributions from long-range, ion–neutral interactions. Recent implementations of the trajectory method have significantly reduced its expense and have made applications to proteins far more tractable. Here, we use ion mobility experiments and trajectory method calculations to characterize the effects of charge state, charge distribution, and structure on the ion mobility of proteins in nitrogen gas. These results show that ion-induced dipole interactions Continue reading “New Publication: Ion Mobility of Proteins in Nitrogen Gas: Effects of Charge State, Charge Distribution, and Structure”
Members of the Bush Lab will present the following talks at American Society for Mass Spectrometry Annual Conference in San Diego (June 3 – 7, 2018):
- Ion Mobility of Proteins in Nitrogen Gas: Effects of Charge State, Charge Distribution, and Structure Characterized using Trajectory Method Calculations. Daniele Canzani; Matthew F, Bush. (MP 407)
- Collision-Induced Unfolding and Dissociation Reveal the Location of Ni(II) Binding in the Dimer of the Alpha-Crystallin Domain of HSPB5. Seoyeon Hong; Matthew F. Bush. (MP 745)
- Memory of the Condensed-Phase in the Gas-Phase: Effects of Solution, Charge, and Energy on Structures of Serum Albumin Ions. Meagan Gadzuk-Shea; Matthew F Bush. (WOB 10:10)
We look forward to seeing everyone in San Diego!
Congratulations to Julia Greenwald, who received the Bruce R. Kowalski Award for Multidisciplinary Research. The award is given to the best lightning talk at the Center for Process Analysis and Control annual meeting.
Congratulations to Rae Eaton, who just received a Graduate Fellowship from the American Chemical Society Division of Analytical Chemistry! This award is sponsored by Agilent Technologies.
Structural characterization of small molecular ions by ion mobility mass spectrometry in nitrogen drift gas: improving the accuracy of trajectory method calculations. Jong Wha Lee, Hyun Hee L. Lee, Kimberly L. Davidson, Matthew F. Bush, Hugh I. Kim. Analyst 2018, in press. (Link)
The investigation of ion structures based on a combination of ion mobility mass spectrometry (IM-MS) experiments and theoretical collision cross section (CCS) calculations has become important to many fields of research. However, the accuracy of current CCS calculations for ions in nitrogen drift gas limits the information content of many experiments. In particular, few studies have evaluated and attempted to improve the theoretical tools for CCS calculation in nitrogen drift gas. In this study, Continue reading “New Publication: Structural characterization of small molecular ions by ion mobility mass spectrometry in nitrogen drift gas”
Our research on “Fundamental Interactions Between Petroleum Ions and Gases” was featured in the most recent annual report from the American Chemical Society Petroleum Research Fund. Congratulations to Kim Davidson and Anna Bakhtina!