Instruments and Other Equipment

Mass Spectrometers

Synapt G2 HDMS

The Bush Lab has a Waters Synapt G2 HDMS quadrupole/ion-mobility/time-of-flight hybrid mass spectrometer. In addition to the standard features, our Synapt G2 is equipped with ion/ion chemistry capabilities, a quadrupole mass filter capable of selecting precursor ions that have m/z of up to 32k, and a radio-frequency confining drift cell developed by our lab to accurately probe the shapes of protein complexes. This instrument enables the characterization of stoichiometry, subunit interactions, and shapes for heterogeneous, dynamic, and/or membrane-bound protein complexes that have masses of up to ~1 MDa.

    • Ion mobility mass spectrometry of peptide, protein, and protein complex ions using a radio-frequency confining drift cell. Samuel J. Allen, Kevin Giles, Tony Gilbert, Matthew F. Bush. Analyst 2016141, 884-891. (Link|PUBMED|PDF)
    • Radio-Frequency (RF) Confinement in Ion Mobility Spectrometry: Apparent Mobilities and Effective Temperatures. Samuel J. Allen, Matthew F. Bush. J. Am. Soc. Mass Spectrom. 201627, 2054–2063. (Link)

Bush Lab Synapt G2 HDMS

Sam Allen and Ken Laszlo using our Synapt G2.
Sam Allen and Ken Laszlo using our Synapt G2.

Structures for Lossless Ion Manipulations

The Bush Lab develops novel ion mobility instrumentation that contains Structures for Lossless Ion Manipulation (SLIM). SLIM are batch-fabricated printed circuits boards that contain planar electrodes for ion confinement and transmission. Individual SLIM boards are implemented as stacked-board pairs that are assembled into modular arrays that enable a variety of ion manipulations, including ion mobility-based separation, selection, and trapping. This instrument enables ion mobility mass spectrometry analysis of native-like proteins and protein complexes.

    • Analysis of Native-Like Ions using Structures for Lossless Ion Manipulations. Samuel J. Allen, Rachel M. Eaton, and Matthew F. Bush. Anal. Chem. 2016, 88, 9118–9126. (Link)
    • Structural Dynamics of Native-Like Ions in the Gas Phase: Results from Tandem Ion Mobility of Cytochrome c. Samuel J. Allen, Rachel M. Eaton, Matthew F. Bush. Anal. Chem. 201789, 7527–7534. (Link)

Waters Synapt G1 HDMS. We have a Waters Synapt HDMS quadrupole/ion-mobility/time-of-flight mass spectrometer. The quadrupole mass filter on this instrument can select precursor ions that have m/z of up to 32k.

Other Equipment

Chromatography. We use various chromatography systems for online and offline separations.

Centrifuges.

Incubators.

Other Resources

Members of the Bush Lab also uses instruments based at the UW Proteomics Resource, the Chemistry Mass Spectrometry Facility, and the Chemistry Spectroscopic and Analytical Instrumentation Facility. These facilities all provide complementary resources that we use to further our research objectives.