Congratulations to Chrissy Stachl (far right), who received one of three ASMS Undergraduate Poster Awards for her poster titled Supercharging of Native-Like Proteins and Protein Complexes: Effects of m-Nitrobenzyl Alcohol versus Sulfolane at this year’s ASMS Conference. Her award was reported in the News and Views section of the August 2014 issue of the Journal of the American Society for Mass Spectrometry.
The Bush Lab and collaborators will present the following talks at the 248th American Chemical Society Fall National Meeting, which will be held in San Francisco (August 10-14):
- Droplet sizes, electrospray currents, and nonspecific aggregation in electrokinetically controlled native nanoelectrospray ionization. Kimberly L Davidson, Derek Oberreit, Christopher J Hogan, Matthew F Bush. (Sunday at 3:10 PM in Moscone Center, North Bldg. Room: 130)
- Native electrospray ionization: From initial droplets to final ions. Matthew F. Bush (Monday at 3:20 PM in Moscone Center, North Bldg. Room: 125)
- Determining masses, separating mixtures, and probing structures of native-like ions using selected cation to anion proton transfer (SCAPT) reactions. Matthew F. Bush, Kenneth J. Laszlo. (Wednesday at 3:30 PM in Moscone Center, North Bldg. Room: 125)
Prof. Bush is also organizing the Advances in Ionization Techniques and Mechanisms Symposium, which will be held on Monday in Moscone Center, North Bldg. Room: 125. Prof. Bush thanks the ACS Division of Analytical Chemistry, Agilent, Bruker, Sutter Instrument, Thermo Scientific, and Waters for the generous sponsorship that made this symposium possible. (Symposium Program | 405 kB)
- Introductory Remarks (8:30)
- Development of Multi-Stage Reactive Transmission Mode Desorption Electrospray Ionization Mass Spectrometry for Characterizing Multicatalytic Reactions. Richard H. Perry, Kevin C. Peters, Kevin E. Parker, Troy J. Comi (8:35)
- Development of Solvent-Free Ambient Mass Spectrometry for Green Chemistry Applications. Hao Chen, Pengyuan Liu, Amanda Forni. (9:05)
- Ambient ionization mass spectrometric imaging with high spatiotemporal resolution. Richard N. Zare, Jae Kyoo Lee, Hong Gil Nam (9:35)
- Intermission (10:05)
- Development of a surface acoustic wave nebulization source for mass spectrometry. David Goodlett, Tao Liang, Scott R Heron, Shivangi Awasthi, Sung H Yoon, Yue Huang, Michael Wilson, David P A Kilgour. (10:05)
- Magic Matrices for Ionization in Mass Spectrometry. Sarah Trimpin. (10:55)
- Low zeptomole detection limits with capillary electrophoresis coupled to an improved nanospray. Norman J. Dovichi, Liangliang Sun, Guijie Zhu (11:25)
- Break for Lunch (11:55)
- Matrix-Assisted Laser Desorption Electrospray Ionization (MALDESI): From Fundamentals to Tissue and Chemical Imaging. David C. Muddiman. (1:30)
- Matrix-free, ambient pressure mass spectrometry via ultra-intense laser vaporization. Robert J. Levis. (2:00)
- Molecular modeling of the ejection mechanisms of macromolecules from charged nanodroplets. Styliani Consta. (2:30)
- Intermission. (3:00)
- Native Electrospray Ionization: From Initial Droplets to Final Ions. Matthew F. Bush. (3:20)
- Charges in protein electrospray ionization: Like or opposite? Rachel R. Ogorzalek Loo, Joseph A. Loo. (3:50)
- Supercharging, Fast Mixing and Charge Detection in Mass Spectrometry. Evan R. Williams. (4:20)
Kim Davidson is also co-organizing the Graduate Student Symposium on International Collaborations with International Impact: Chemistry for Global Change, which will be held on Tuesday in the Esplanade Ballroom 304 in the south building of the Moscone Center.
We look forward to seeing everyone in San Francisco!
Prof. Bush received a grant from the American Chemical Society Petroleum Research Fund (ACS PRF) through the Doctoral New Investigator program. Our group is grateful for this generous financial support.
The Bush Lab is a research group in the Department of Chemistry and the Biological Physics, Structure & Design Program at the University of Washington. Our research focuses on the development and application of mass spectrometry and ion mobility spectrometry techniques to elucidate the structures and assembly of protein complexes and subcellular machines.
- Interested in joining the Bush Lab, click here.
Erin and I hope that everyone enjoyed this year’s workshop! Mike Bowers, Colin Creaser, and Herb Hill have kindly agreed to share their slides (links below).
Applying Ion Mobility to Biological Problems
Erin Baker (PNNL) and Matt Bush (University of Washington), presiding
5:45 PM on Monday, June 16th. Room 314-317.
Results from ion mobility mass spectrometry studies are increasingly used to answer questions in biology, including applications to metabolomics, proteomics, targeted interactions, and large molecules. This is in part attributed to the increasing performance and selection of commercial ion mobility mass spectrometry instrumentation, which has made it easier to integrate ion mobility technologies into mass spectrometry workflows. In this workshop, 3 members of the community will present their perspectives on the future of ion mobility MS in biology. These perspectives will be followed by a panel discussion of the challenges that arise in different types of ion mobility studies, what is possible today, and opportunities for the future.
Perspectives on the Future of Ion Mobility MS
- Michael Bowers (University of California, Santa Barbara) (Slides | PDF | 2.3 mb)
- Colin Creaser (Loughborough University) (Slides | PDF | 220 kb)
- Herb Hill (Washington State University) (Slides | PDF | 5.9 mb)
- Michael Bowers (University of California, Santa Barbara)
- Colin Creaser (Loughborough University)
- Kevin Giles (Waters)
- Michael Grössl (TOFWERKS)
- Herb Hill (Washington State University)
- Mel Park (Bruker)
- Brad Schneider (AB SCIEX)
- George Stafford (Agilent)
- Danielle Toutoungi (Owlstone)
Please send questions or suggestions for the panel discussion to Erin Baker (Erin [dot] Baker [at] pnnl [dot] gov) or Matt Bush (mattbush [at] uw [dot] edu). We look forward to seeing everyone in Baltimore!
Chrissy Stachl has been selected to lead degree candidates from the College of Arts and Sciences at this year’s Commencement. From the University of Washington:
Each year the various schools and colleges select a student or students to lead their degree candidates in the procession at the University of Washington’s Commencement Ceremony. These students are called gonfalonieres because they carry the school’s gonfalon, a banner that hangs down from a crosspiece and bears that school’s name and symbol.
To learn more about the Gonfalonieres and Chrissy, click here.
Chrissy Stachl will present the following talk at the 17th Annual Undergraduate Research Symposium on Friday, May 16th:
Supercharging of Native-Like Proteins and Protein Complexes: Effects of m-Nitrobenzyl Alcohol versus Sulfolane
Chrissy Stachl, Samuel Allen, Matthew Bush
Session 1U | Chemistry of Materials and Biomolecules | 12:30 PM to 2:15 PM | 175 JHN
The Bush Lab and collaborators will present the following talks and posters at American Society for Mass Spectrometry Annual Conference in Baltimore, MD (June 15-19):
- Determining Masses, Separating Mixtures, and Probing Structures of Native-Like Ions Using Selected Cation to Anion Proton Transfer (SCAPT) Reactions. Laszlo, K. J.; Bush, M. F. (MOD 9:30-9:50)
- Supercharging of Native Protein Complexes: Effects of Polarity and Evidence for Multiple Mechanisms. Allen, S. J.; Stachl, C. N.; Bush, M. F. (MOC 3:30-3:50)
- Supercharging of Native-Like Proteins and Protein Complexes: Effects of m-Nitrobenzyl Alcohol versus Sulfolane. Stachl, C. N.; Allen, S. J.; Bush, M. F. (WP669)
- Probing Protein-Protein and Protein-DNA Interactions by Native Mass Spectrometry and Global Hydrogen Deuterium Exchange using Surface Acoustic Wave Nebulization (SAWN). Monkkonen, L.; Heron, S.; Bush, M. F.; Catalano, C. E.; Goodlett, D. R. (ThP93)
- Additional Models of a Mammalian Cryptochrome – E3 Ubiquitin Ligase Complex Restrained by Results from Ion Mobility Mass Spectrometry. Marionni, S. T.; Xing, W.; Zheng, N.; Bush, M. F. (ThP470)
- Droplet sizes, electrospray currents, and nonspecific aggregation in electrokinetically controlled native nanoelectrospray ionization. Davidson, K. L.; Oberreit, D. R.; Hogan, C. J.; Bush, M. F. (ThOC 4:10-4:30)
Matt Bush and Erin Baker (PNNL) are also organizing the “Applying Ion Mobility to Biological Problems Workshop”, which is organized by the Ion Mobility MS Interest Group. The workshop will be held on Monday from 5:45-7:00 pm in Rooms 314-317. Click here for more details.
We look forward to seeing everyone in Baltimore!
Sam Allen will present the following poster and talk at the US HUPO 10th Annual Conference: Frontiers in Proteomics which takes place at The Westin Seattle Hotel in Seattle, WA from April 6-9, 2014.
- Manipulating the Charge States of Native-Like Proteins and Protein Complexes: Charge Reduction, Supercharging, and Insights into Electrospray Mechanisms Samuel J. Allen, Alicia M. Schwartz, Christiane N. Stachl, Matthew F. Bush (Poster #082, Lightning Talk April 8 2:25 pm)