New Publication: Effects of Solution Structure on the Folding of Lysozyme Ions in the Gas-Phase

Effects of Solution Structure on the Folding of Lysozyme Ions in the Gas-Phase. Kenneth J. Laszlo, Eleanor B. Munger, Matthew F. Bush. J. Phys. Chem. B 2017, 121, 2759–2766. (Link)

The fidelity between the structures of proteins in solution and protein ions in the gas phase is critical to experiments that use gas-phase measurements to infer structures in solution. Here we generate ions of lysozyme, a 129-residue protein whose native tertiary structure contains four internal disulfide bonds, from three solutions that preserve varying extents of the original native structure. We then use cation-to-anion proton-transfer reactions (CAPTR) to reduce the charge states of those ions in the gas phase and ion mobility to probe their structures. The collision cross section (Ω) distributions of Continue reading

Congratulations to Julia Greenwald, who was awarded an NSF Graduate Research Fellowship!

Julia Greenwald, a first year graduate student in the Bush Lab, was recently awarded a National Science Foundation Graduate Research Fellowship! From the NSF:

The NSF Graduate Research Fellowship Program recognizes and supports outstanding graduate students in NSF-supported science, technology, engineering, and mathematics disciplines who are pursuing research-based Master’s and doctoral degrees at accredited United States institutions. (For additional information, click here.)

Congratulations to Sam Allen, who just defended his dissertation!

Prof. Bush to receive the 2017 Arthur F. Findeis Award

Prof. Bush has been named the recipient of the 2017 Arthur F. Findeis Award for Achievements by a Young Analytical Scientist. The Findeis Award is given annually by the American Chemistry Society Division of Analytical Chemistry, to recognize and encourage outstanding contributions to the fields of analytical chemistry by a young analytical scientist. The award will be presented at the 254th ACS National Meeting to be held August 20-24, 2017 in Washington DC. (Link to additional information about the award)

New Publication: Native-Like and Denatured Cytochrome c Ions Yield Cation-to-Anion Proton Transfer Reaction Products with Similar Collision Cross-Sections


Native-like and Denatured Cytochrome c Ions Yield Cation-to-Anion Proton-Transfer Products with Similar Collision Cross Sections. Kenneth J. Laszlo, John H. Buckner, Eleanor B. Munger, Matthew F. Bush. J. Am. Soc. Mass Spectrom. 2017, in press. (Link)

The relationship between structures of protein ions, their charge states, and their original structures prior to ionization remains challenging to decouple. Here, we use cation-to-anion proton transfer reactions (CAPTR) to reduce the charge states of cytochrome c ions in the gas phase, and ion mobility to probe their structures. Ions were formed using a new temperature-controlled nanoelectrospray ionization source Continue reading

New Lab Member: Daniele Canzani

The Bush Lab welcomes Daniele Canzani! Click here to learn more about Daniele.

New Publication: Effects of drift gas selection on the ambient-temperature, ion mobility mass spectrometry analysis of amino acids

Effects of drift gas selection on the ambient-temperature, ion mobility mass spectrometry analysis of amino acids. Kimberly L. Davidson and Matthew F. Bush. Anal. Chem. 2017, DOI: 10.1021/acs.analchem.6b04605. (Link)

Ion mobility (IM) separates ions based on their response to an electric field in the presence of a drift gas. Due to its speed and sensitivity, the integration of IM and mass spectrometry (MS) offers many potential advantages for the analysis of small molecules. To determine the effects that drift gas selection has on the information content of IM separations, absolute collision cross sections (Ω) with He, N2, Ar, CO2, and N2O were measured for the 20 common amino acids using low-pressure, ambient-temperature ion mobility experiments performed in a radio-frequency-confining drift cell. Continue reading

Analyst Most Accessed Articles in 2016

Congratulations to Sam Allen, whose article was one of the top 25 most downloaded articles published in the Analyst in 2016. That article is now featured in the Analyst 2016 Most Accessed Articles Collection.

  • Ion mobility mass spectrometry of peptide, protein, and protein complex ions using a radio-frequency confining drift cell. Samuel J. Allen, Kevin Giles, Tony Gilbert, Matthew F. Bush. Analyst 2016141, 884-891. (Link|PUBMED|PDF)

New Instrument

We are fabricating a new instrument for ion mobility mass spectrometry, which was designed by Sam Allen and Rae Eaton. Here is a video of some recent work by the machine shop in UW Department of Chemistry.

New Lab Member: Julia Greenwald

The Bush Lab welcomes Julia Greenwald! Click here to learn more about Julia.