Sam Allen

Mobility-Cell_edit

Contact: sjallen [at] uw [dot] edu

Bio

  • Sam Allen graduated with honors from California State University Chico, obtaining a B.S. in Chemistry and a minor in Physics and Mathematics. In his undergraduate research with Prof. Lisa Ott, HPLC and GC-MS techniques were used to investigate almond nut oil as an alternative feedstock for the preparation of biodiesel fuel. Sam also obtained a Summer Undergraduate Research Fellowship (SURF) at the National Institute of Standards and Technologies (NIST) in Boulder, CO where he worked with Dr. Thomas J. Bruno on “Prediction and Standardization of Fire Debris Evaporation Patterns with the Advanced Distillation Curve Method”. Sam is continuing his studies in Prof. Matthew Bush’s lab at the University of Washington.

Research Interests

  • Development of New Ion Mobility Devices. A new RF-confining drift tube was developed to study the structure of biomolecular species. This device was implemented in a Waters Synapt G2 HDMS which controls all voltages and regulates gas flow. The RF-confining drift tube can be used to directly determine collision cross section values of unknown species, without the use of drift time calibration.
  • Cation and Anion Structure. Using an RF-confining drift tube, collision cross section values of small molecule, polymer, and protein complex anions were determined. These values can be used for anion traveling-wave calibrations. Currently, I am using charge manipulation methods and ion mobility to compare the structures of anions and cations in the gas phase.
  • Ion Transport Fundamentals. Using the ion optics package SIMION, I am investigating ion transport in the gas phase. Current work has been focused on the effects of radial confinement on the mobility of ions.

Awards

  • ACS Division of Analytical Chemistry Graduate Fellowship, 2014.
  • Graduate Student Merit Fellowship, UW Department of Chemistry, 2013.
  • 1st Prize, Poster Competition, Cascadia Proteomics Symposium, Seattle, WA, 7/16/2013.
  • Richard A. Schaeffer Memorial Fund Travel Award, 61st American Society for Mass Spectrometry Conference, 2013.
  • Gordon Research Conference Chair Travel Award, Gaseous Ions: Structures, Energetics & Reactions, 2013.
  • 2nd Prize, Poster Competition, Cascadia Proteomics Symposium, Seattle, WA, 7/21/2012. Award Info

Publications

Oral Presentations

  • Allen, S. J.; Stachl, C. N.; Bush, M. F. “Supercharging of Native Protein Complexes: Effects of Polarity and Evidence for Multiple Mechanisms” 62nd American Society for Mass Spectrometry Conference, Baltimore, MD, 6/2014.
  • Allen, S. J.; Schwartz, A. M.; Stachl, C. N.; Bush, M. F. “Manipulating the Charge States of Native-Like Proteins and Protein Complexes: Charge Reduction, Supercharging, and Insights into Electrospray Mechanisms” US HUPO, Seattle, WA , 4/8/2014 (Lightning Talk).
  • Allen, S. J.; Schwartz, A. M.; Bush, M. F. “Investigation of Protein Charging and Structure using a New Ion Mobility Device” Cascadia Proteomics Symposium, Seattle, WA, 7/16/2013.
  • Allen, S. J.; Marionni, S. T.; Giles, K.; Gilbert, T.; Bush, M. F. “Design and Characterization of a New Ion Mobility Cell for Protein Complexes” Cascadia Proteomics Symposium, Seattle, WA, 7/21/2012.

Poster Presentations

  • Allen, S. J.; Schwartz, A. M.; Stachl, C. N.; Bush, M. F. “Manipulating the Charge States of Native-Like Proteins and Protein Complexes: Charge Reduction, Supercharging, and Insights into Electrospray Mechanisms” US HUPO, Seattle, WA , 4/8/2014.
  • Allen, S. J.; Schwartz, A. M.; Bush, M. F. “Ion Mobility Mass Spectrometry of Native Protein Complex Anions” Cascadia Proteomics Symposium, Seattle, WA, 7/16/2013.
  • Allen, S. J.; Schwartz, A. M.; Bush, M. F. “Ion Mobility Mass Spectrometry of Native Protein Complex Anions” 61st American Society for Mass Spectrometry Conference, Minneapolis, MN, 6/2013.
  • Allen, S. J.; Bush, M. F. “The Effects of Radial Confinement on the Apparent Mobility of Ions in Gases” GRC: Gaseous Ions: Structures, Energetics & Reactions, Galveston, TX, 2/27/2013.
  • Allen, S. J.; Marionni, S. T.; Giles, K.; Gilbert, T.; Bush, M. F. “Design and Characterization of a New Ion Mobility Cell for Protein Complexes” PACMASS Members’ Night, Seattle, WA, 10/29/2012.
  • Allen, S. J.; Marionni, S. T.; Giles, K.; Gilbert, T.; Bush, M. F. “Design and Characterization of a New Ion Mobility Cell for Protein Complexes” Cascadia Proteomics Symposium, Seattle, WA, 7/19/2012.
  • Allen, S. J.; Marionni, S. T.; Giles, K.; Gilbert, T.; Bush, M. F. “Design and Characterization of a New Ion Mobility Cell for Protein Complexes” 60th American Society for Mass Spectrometry Conference, Vancouver, BC, 5/2012.
  • Marionni, S. T.; Allen, S. J.; Bush, M. F. “Gas-Phase Structural Biology of Proteins and their Complexes” Biological Physics, Structure and Design Retreat, Seattle, WA, 11/28/11.
  • Marionni, S. T.;* Allen, S. J.; Bush, M. F. “Gas-Phase Structural Biology of Proteins and their Complexes” PACMASS Members’ Night, Seattle, WA, 11/8/11.

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