NK Cell Functional Assays

  • NK cell immunophenotyping: can include inhibitory and activating receptors for NK cells.
  • Viral Suppression Assay (VSA): Measures the ability of NK cells to inhibit viral replication in autologous CD4 cells. This inhibition measures a combination of both direct cytotoxicity and chemokine/cytokine secretion.
  • Cytotoxicity Assay: Measures the direct killing activity of NK cells when they encounter HIV-infected targets. This is a flow-based assay.
  • Antibody-dependent Cell-mediated Viral Inhibition (ADCVI): Measures the ability of HIV-specific antibodies to mediate killing of HIV-infected cells via NK cell activation. Assay can be performed with autologous or heterologous NK cells.

For more information on how to access these services, contact Ana Gervassi at ana.gervassi@cidresearch.org