When designing a flow cytometry experiment, it is often useful to take advantage of prior experience in terms of combinations of colors and markers that work well. We have worked with markers to examine most leukocyte cell types in blood. In addition to cell phenotyping, we have experience measuring cell function using the intracellular cytokine staining assay. We hope that our expertise can help streamline the design of your experiments.
For more information on how to access this service, contact Stephen De Rosa at sderosa at fhcrc.org.