Inflammation Core Services
The Inflammation Core is located within the Center for Lung Biology (CLB) at the South Lake Union (SLU) campus of the University of Washington School of Medicine. Inflammation Core provides support to assess endpoints and processes of inflammation and leukocyte activity relevant to CF. The Inflammation Core will utilize the CLB’s Histology and Imaging Core to provide a full range of automated histological services, digital microscopy, and veterinary pathology. In addition, the Inflammation Core will use the Flow Cytometry/FACS Core in the Institute for Stem Cell and Regenerative Medicine at SLU. The primary services of Core C will be:
- Routine histology, immunohistochemistry, and in situ hybridization.
- State-of-the-art imaging modalities, data storage, and morphometric analysis.
- Assays of the host response to infection, inflammatory factors, and leukocyte function.
- Flow cytometry, cell sorting, and analysis.
- Expertise, consultation, and training in comparative pathology.
- Training and consultation in all technologies available through this core.
- Leukocyte differentials in tissue and fluid samples.
- Quantification of chemokines, cytokines, and other factors.
- Imaging assays to localize sites of leukocyte influx and identify cell types.
- In vivo infection assay to assess host response to clinical isolates.
- Live-cell imaging approaches and morphometric analyses.
- Cytometric assays to quantify, isolate, and phenotype specific cell types.
- Assays on leukocyte function and activation status.