Norman J. Dovichi

Representative Publications

Single cell analysis

 “Cell cycle-dependent characterization of single MCF-7 breast cancer cells by 2-D CE.” Harwood MM, Bleecker JV, Rabinovitch PS, Dovichi NJ.  Electrophoresis; 28: 932-937 (2007).

 “Single-cell protein analysis of a single mouse embryo by two-dimensional capillary electrophoresis.” Harwood MM, Christians ES, Fazal MA, Dovichi NJ. J Chromatogr A.; 1130: 190-4 (2006).

 “Repeatability of chemical cytometry: 2-DE analysis of single RAW 264.7 macrophage cells.” Sobhani K, Fink SL, Cookson BT, Dovichi NJ. Electrophoresis. 2007; 28: 2308-13. “Analysis of differential detergent fractions of an AtT-20 cellular homogenate using one- and two-dimensional capillary electrophoresis.” Fazal MA, Palmer VR, Dovichi NJ.  J Chromatogr A.; 1130: 182-9 (2006).

"Capillary sieving electrophoresis-micellar electrokinetic chromatography fully automated two-dimensional capillary electrophoresis analysis of Deinococcus radiodurans protein homogenate.", D.A. Michels, S. Hu, K.A. Dambrowitz, M.J. Eggertson, K. Lauterbach, N.J. Dovichi, Electrophoresis, 25 3098 (2004).

"Capillary sieving electrophoresis/micellar electrokinetic capillary chromatography for two-dimensional protein fingerprinting of single mammalian cells.", S. Hu, D. Michels, M. A. Fazal, C. Ratisoontorn, M.L. Cunningham, N.J. Dovichi, Anal. Chem., 76 4044 (2004)

"Cell-cycle dependent protein fingerprint from a single cancer cell: image cytometry coupled with single-cell capillary sieving electrophoresis.", S. Hu, L. Zhang, S. N. Krylov, N. J. Dovichi, Anal. Chem., 75 3495 (2003). Click here to download a reprint of this article

"Identification of proteins in single-cell capillary electrophoresis fingerprints based on co-migration with standard proteins.", S. Hu, L. Zhang, R. Newitt, R. Aebersold, J. R. Kraly, M. Jones, N. J. Dovichi, Anal. Chem., 75 3502 (2003). Click here to download a reprint of this article

"Capillary SDS-DALT electrophoresis with laser-induced fluorescence for size-based analysis of proteins in human colon cancer cells.", S. Hu, J. Jiang, L. Cook, D. P. Richards, L. Horlick, B.Wong, and N. J. Dovichi, Electrophoresis, 23 3136 (2002). Click here to download a reprint of this article

"Fully automated two-dimensional capillary electrophoresis for high-sensitivity protein analysis.", D. Michels, S. Hu, R. Schoenherr, M. J. Eggertson, N. J. Dovichi, Mol. Cell. Proteomics, 1 69 (2002). Click here to download a reprint of this article

"Protein analysis of an individual Caenorhabditis elegans single-cell embryo by capillary electrophoresis.", S. Hu, R. Lee, Z. Zhang, S. N. Krylov, and N. J. Dovichi, J. Chrom., 752 307 (2001).

"One-dimensional protein analysis of an HT29 human colon adenocarcinoma cell.", Z. Zhang, S. Krylov, E. A. Arriaga, R. Polakowski, and N. J. Dovichi, Anal. Chem., 72 318 (2000).

"Correlating cell cycle with metabolism in single cells: the combination of image and metabolic cytometry.", S. N. Krylov, Z. Zhang, N.W. C. Chan, E. Arriaga, M. M. Palcic, and N.J. Dovichi, Cytometry, 37 15 (1999). Click here to download a reprint of this article

DNA sequencing and analysis

"Two-Dimensional Direct-Reading Fluorescence Spectrograph for DNA Sequencing by Capillary Array Electrophoresis.", J. Zhang, M. Yang, X. Puyang, Y. Fang, L. M. Cook, N. J. Dovichi, Anal. Chem., 73 1234 (2001).

"How capillary electrophoresis sequenced the human genome.", N. J. Dovichi and J. Z. Zhang, Angew. Chem. Int. Ed., 39 4463 (2000).

"Capillary array DNA sequencer based on a micromachined sheath-flow cuvette.", H. John Crabtree, S. J. Bay, D. F. Lewis, L. D. Coulson, G. Fitzpatrick, D. J. Harrison, S. L. Delinger, J. Z. Zhang, and N. J. Dovichi, Electrophoresis, 21 1329 (2000).

"A multiple-capillary electrophoresis system for small-scale DNA sequencing and analysis.", J. Z. Zhang, K. O. Voss, D. F. Shaw, K. P. Roos, D. F. Lewis, J. Yan, R. Jiang, H. Ren, J. Y. Hou, Y. Fang, X. Puyang, H. Ahmadzadeh, and N. J. Dovichi, Nuc. Acids Res., 27 E36 (1999).

"The limiting mobility of DNA sequencing fragments for both cross-linked and noncross-linked polymers in capillary electrophoresis: DNA sequencing at 1200 Vcm-1.", J. Y. Yan, N. Best, J. Z. Zhang, H. J. Ren, R. Jiang, J. Hou, and N. J. Dovichi, Electrophoresis, 17 1037 (1996).

"Three DNA sequencing methods using capillary gel electrophoresis and laser-induced fluorescence.", H. Swerdlow, J. Z. Zhang, D. Y. Chen, H. R. Harke, R. Grey, S. Wu, C. Fuller, and N. J. Dovichi, Anal. Chem., 63 2835 (1991).

"Capillary gel electrophoresis for DNA sequencing: laser-induced fluorescence detection with the sheath flow cuvette.", H. P. Swerdlow, S. Wu , H. R. Harke, and N. J. Dovichi, J. Chrom., 516 61 (1990).

Ultrasensitive analysis

 “Yoctomole Analysis Of Ganglioside Metabolism in PC12 Cellular Homogenates” Whitmore CD, Olsson U, Larsson EA, Hindsgaul O, Palcic M, Dovichi NJ Electrophoresis.; 28: 3100-4 (2007).

"Single Molecule Enzymology.", N. J. Dovichi, R. Polakowski, A. Skelley, D. B. Craig, J. Wong, Nobel Conference on Single Molecule Spectroscopy in Physics, Chemistry and Biology; Basche, Orrit and Rigler, Eds. Springer: Berlin, 14 242 (2001).

"Single molecules of highly purified bacterial alkaline phosphatase have identical activity.", R. Polakowski, D. Craig, A. Skelley, N. J. Dovichi, J. Am. Chem. Soc., 122 4853 (2000).

"The life and death of a single enzyme molecule.", D. B. Craig, E. Arriaga, J. C. Y. Wong, H. Lu, and N. J. Dovichi, Anal. Chem., 70 39A (1998).

"Single-molecule detection in capillary electrophoresis: molecular shot noise as a fundamental limit to chemical analysis.", D. Y. Chen and N. J. Dovichi, Anal. Chem. 68 690 (1996).

“Subattomole amino acid analysis by capillary zone electrophoresis and laser induced fluorescence.”, Y. F. Cheng, N. J. Dovichi, Science, 242 562 (1988).

"Laser-induced fluorescence of flowing samples as an approach to single-molecule detection in liquids.", N. J. Dovichi, J. C. Martin, J. H. Jett, M. Trkula, and R. A. Keller, Anal. Chem., 56 348 (1984).

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