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Heinz G. Floss

Representative Publications

S. C. Wenzel, R. M. Williamson, C. Grünanger, J. Xu, K. Gerth, R. A. Martinez, S. J. Moss, B. J. Carroll, S. Grond, C. J. Unkefer, R. Müller, H. G. Floss, "On the Biosynthetic Origin of Methoxymalonyl-Acyl Carrier Protein, the Substrate for Incorporation of "Glycolate" Units Into Ansamitocin and Soraphen A", J. Am. Chem. Soc. 128, 14325-14336 (2006).

B. Srinivasulu, Y. - J. Kim, Y. - K. ChangT. - W. Yu, H. G. Floss, "Construction of asm2 Deletion Mutant of Actinosynnema pretiosum and Medium Optimization for Ansamitocin P-3 Production Using Statistical Approach" J. Microbiol. Biotechnol. 16, 1338-1346 (2006).

Y. Hu, H. G. Floss, "Starter Unit Specificity of the Asukamycin "Upper" Chain Polyketide Synthase and the Branched-Chain Fatty Acid Synthase of Streptomyces nodosus subsp. asukaensis" Heterocycles, 69, 133-149 (2006).

T. Kubota, M. Brünjes, T. Frenzel, J. Xu, A. Kirschning, H. G. Floss, "Determination of the Cryptic Stereochemistry of the First PKS Chain Extension Step in Ansamitocin Biosynthesis by Actinosynnema pretiosum" ChemBiochem, 7, 1221-1225 (2006).

L. Bai, L. Li, H. Xu, K. Minagawa, Y. Yu, Y. Zhang, H. G. Floss, X. Zhou, T. Mahmud, Z. Deng, "Functional Analysis of the Validamycin Biosynthetic Gene Cluster and Engineered Production of Validoxylamine A", Chem. Biol., 13, 387-397 (2006).

H. G. Floss, "From Ergot to Ansamycins - 45 Years in Biosynthesis", J. Nat. Prod. 69, 158-169 (2006).

H. G. Floss, "Combinatorial Biosynthesis - Potential and Problems", J. Biotechnol. 124, 242-257 (2006).

J. Xu, E. Wan, C. - J. Kim, H. G. Floss, T. Mahmud, "Identification of Tailoring Genes Involved in the Modification of the Polyketide Backbone of Rifamycin B by Amycolatopsis mediterranei", Microbiology SGM 151, 2515-2528 (2005).

Y. Yu, L. Bai, K. Minagawa, X. Jian, L. Li, J. Li, S. Chen, E. Cao, T. Mahmud, H. G. Floss, X. Zhou, Z. Deng, "A gene cluster encoding validamycin biosynthesis in Streptomyces hygroscopicus var. jinggangensis 5008", Appl. Environ. Microbiol.71, 5066-5076 (2005).

Z. Liu, H. G. Floss, J. M. Cassady, K. K. Chan, "Metabolism studies of the anti-tumor agent maytansine and its analog ansamitocin P-3 using liquid chromatography/tandem mass spectrometry" J. Mass Spect. 40, 389-399 (2005).

H. G. Floss, T. - W. Yu, "Rifamycin - Mode of Action, Resistance and Biosynthesis" Chem. Rev. 105, 621-632 (2005).

Z. Liu, H. G. Floss, J. M. Cassady, J. Xiao, K. K. Chan, "An API LC/MS/MS Quantitation Method for Ansamitocin P-3 (AP3) and Its Preclinical Pharmacokinetics" J. Pharm. Biomed. Anal. 36, 815-821 (2004)

Y. Hu, H. G. Floss, "Further Studies on the Biosynthesis of the Manumycin Type Antibiotic, Asukamycin, and the Chemical Synthesis of Protoasukamycin" J. Am. Chem. Soc. 126, 3837-3844 (2004)

T.-W. Yu, H. G. Floss, "Ansamitocins (Maytansinoids)" , Ch. 17 in "Antitumor Agents from Natural Products", G. M. Cragg, D. J. Newman, D. G. I. Kingston, eds., Taylor & Francis, Boca Raton, Florida, 2005, pp 321-337.

J. M. Cassady, K. K. Chan, H. G. Floss, E. Leistner, "Recent Developments in the Maytansinoid Antitumor Agents" Chem. Pharm. Bull. 52, 1-26 (2004).

"Isolation and characterization of 27-O-demethylrifamycin SV methyltransferase provides new insights into the post-PKS modification steps during the biosynthesis of the antitubercular drug rifamycin B by Amycolatopsis mediterranei S699.", J. Xu, T. Mahmud, and H. G. Floss,  Arch. Biochem. Biophys.,  411 277 (2003).

“Occurrence and Non-detectability of Maytansinoids in Individual Plants of the Genera Maytenus and Putterlickia.”, C. B. Pullen, P. Schmitz, D. Hoffmann, K. Meurer, T. Boettcher, D. v. Bamberg, A. M. Pereira, S. de Castro França, M. Hauser, H. Geertsema, A.van Wyk, T. Mahmud, H. G. Floss, E. Leistner, Phytochemistry, 62 377 (2003).

"Characterization of the Early Stage Aminoshikimate Pathway in the Formation of 3-Amino-5-hydroxybenzoic Acid (AHBA): The RifN Protein Specifically Converts Kanosamine into Kanosamine 6-Phosphate.", K. Arakawa, R. Müller, T. Mahmud, T.–W. Yu, H. G. Floss, J. Am. Chem. Soc., 124 10644 (2002). Click here to download a reprint of this article

"Identification of a Set of Genes Involved in the Formation of the Substrate for the Incorporation of the Unusual 'Glycolate' Chain Extension Unit in Ansamitocin Biosynthesis.", B. J. Carroll, S. J. Moss, L. Bai, Y. Kato, S. Toelzer, T.-W. Yu, H. G. Floss, J. Am. Chem. Soc., 124 4176 (2002).

"The biosynthetic gene cluster of the maytansinoid antitumor agent ansamitocin from Actinosynnema pretiosum.", T.–W. Yu, L. Bai, D. Clade, D. Hoffmann, S. Toelzer, K. Q. Trinh, J. Xu, S. J. Moss, E. Leistner, and H. G. Floss, Proc. Nat. Acad. Sci. USA, 99 7968 (2002). Click here to download a reprint of this article

"Functional Expression of Genes Involved in the Biosynthesis of the Novel Polyketide Chain Extension Unit, Methoxymalonyl-Acyl Carrier Protein, and Engineered Biosynthesis of 2-Desmethyl-2-Methoxy-6-Deoxyerythronolide B.", Y. Kato, L. Bai, Q. Xue, W. P. Revill, T.-W. Yu, H. G. Floss, J. Am. Chem. Soc., 124 5268 (2002). Click here to download a reprint of this article

"Biosynthesis of the Antibiotic Echinosporin by a Novel Branch of the Shikimate Pathway.", A. Dübeler, P. Krastel, H. G. Floss, A. Zeeck, Eur. J. Org. Chem., 254 983 (2002).

"Identification of Asm19 as an Acyltransferase Attaching the Biologically Essential Ester Side Chain of Ansamitocins Using N-Desmethyl-4,5-desepoxymaytansinol, not Maytansinol, as its Substrate.", S. J. Moss, L. Bai, S. Toelzer, B. J. Carroll, T. Mahmud, T.-W. Yu, H. G. Floss, J. Am. Chem. Soc., 124 6544 (2002).

"Biosynthetic Studies on the a-Glucosidase Inhibitor Acarbose: The Chemical Synthesis of dTDP-4-amino-4,6-dideoxy-a-D-Glucose.", S. G. Bowers, T. Mahmud, H. G. Floss, Carbohydr. Res., 337 297 (2002).

"Mutational Analysis and Reconstituted Expression of the Biosynthetic Genes Involved in the Formation of 3-Amino-5-hydroxybenzoic Acid, the Starter Unit of Rifamycin Biosynthesis in Amycolatopsis mediterranei S699.", T.– W. Yu, R. Müller, M. Müller, H. Zhang, G. Draeger, C.– G. Kim, E. Leistner, H. G. Floss, J. Biol. Chem., 276 12546 (2001). Click here to download a reprint of this article

"Biosynthesis of the Validamycins: Identification of Intermediates in the Biosynthesis of Validamycin A by Streptomyces hygroscopicus var. limoneus.", H. Dong, T. Mahmud, I. Tornus, S. Lee, H. G. Floss, J. Am. Chem. Soc., 123 2733 (2001).

"Phenazine Biosynthesis in Pseudomonas fluorescens: Branchpoint from the Primary Shikimate Biosynthetic Pathway and Role of Phenazine-1,6-dicarboxylic Acid." M. McDonald, D. V. Mavrodi, L. S. Thomashow, H. G. Floss. J. Am. Chem. Soc., 123 9459 (2001).

"New Type II Manumycins Produced by Streptomyces nodosus ssp. asukaensis and Their Biosynthesis.", Y. Hu, H. G. Floss, J. Antibiot, 54 340 (2001).

"Intramolecular proton transfer in the cyclization of geranylgeranyl diphosphate to the taxadiene precursor of taxol catalyzed by recombinant taxadiene synthase.", D. C. Williams, B. J. Carroll, Q. Jin, C. Rithner, S. R. Lenger, H. G. Floss, R. M. Coates, R. M. Williams, R. Croteau, Chem. & Biol., 45 1 (2000).

"Direct evidence that the rifamycin polyketide synthase assembles polyketide chains processively.", T.-W. Yu, Y. Shen, Y. Doi-Katayama, L. Tang, C. Park, B. S. Moore, C. R. Hutchinson, H. G. Floss, Proc. Nat. Acad. Sci. USA, 96 9051 (1999). Click here to download a reprint of this article

"Ectopic Expression of the Minimal whiE Polyketide Synthase Generates a Library of Aromatic Polyketides of Diverse Sizes and Shapes.", Y. Shen, P. Yoon, T.-W. Yu, H. G. Floss, D. Hopwood, B. S. Moore, Proc. Nat. Acad. Sci. USA, 96 3622 (1999).

"The AcbC Protein from Actinoplanes sp. Is a C7-Cyclitol Synthase Related to 3-Dehydroquinate Synthases and Is Involved in the Biosynthesis of the alpha-Glucosidase Inhibitor Acarbose.", A. Stratmann, T. Mahmud, S.-S. Lee, J. Distler, H. G. Floss, W. Piepersberg, J. Biol. Chem., 274 10889 (1999).

"Mechanism of the 2-Deoxygenation Step in the Biosynthesis of the Deoxyhexose Moieties of the Antibiotics Granaticin and Oleandomycin.", G. Draeger, S.-H. Park, H. G. Floss, J. Am. Chem. Soc., 121 2611 (1999).

Back to Research Interest

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Awards & Activities

Elected Honorary Member of the Kitasato Institute, Tokyo

Honorary Doctor of Science Degree, University of Bonn

White Magnolia Commemoration Award and Medal, City of Shanghai

Research Achievement Award, American Scociety of Pharmacognosy

Honorary Doctor of Science Degree, Purdue University

Humboldt Senior Scientist Award

Volwiler Award, American Association of Colleges of Pharmacy

Research Achievement Award in Natural Products, Academy of Pharmaceutical Sciences

 

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