Multiple Investigators within CIBR have vast experience in optical imaging technology. As such, CIBR has committed extensive resources into building and developing a state-of-the-art Imaging Core. We are focused on advancing systems and techniques for high-resolution anatomical and physiological analyses of both fixed/stained tissues and sections, and in particular live organotypic brain slices in culture, and whole live-animal brain imaging. We have several high-resolution fluorescent microscopes that serve as our foundation for conducting detailed immunocytochemical analyses of both sectioned and whole-mounted tissues including a Zeiss Apotome with motorized XYZ-stage, a Zeiss 710 34-channel Quasar Laser Scanning Confocal Microscope, and two Olympus IX81-ZDC DSU Dual View Imaging Systems with motorized xyz-drive stages. We have several multiphoton microscope systems dedicated to high-resolution spatiotemporal live-cell imaging. As the 1st of its kind in the world, our Dual Olympus FV1000’s Split-Beam DeepSea Multiphoton Microscope is capable of simultaneously imaging two samples, capitalizing on identical beam-paths and dual Ultra-25X NA 1.05 objectives. Furthermore, one Olympus FV1000 is outfitted as a visible light Laser Scanning Confocal Microscope equipped with extra- and intracellular electrophysiological recording devices and optical/e-phys Mapping Software. Additionally, we have a Trim Scope II Multibeam Multiphoton Microscope from LaVision Biotech that serves as our basis for live whole-animal brain imaging. We have off-line image analysis workstations equipped with MetaMorph, Imaris, and FarSight software suites. This Imaging Core resource allows CIBR investigators access to the most cutting edge techniques in neuroscience basic and clinical research, such as optogenetics. We can assist other CIBR and SCRI investigators in experimental design and analysis.
Click Here For Movies from Nelson et al. (2013): Dynamic interactions between Intermediate Neurogenic Progenitors and Radial Glia in embryonic mouse neocortex: potential role in Dll1-Notch signaling.