NMR
Agilent DD2 500 MHz
The Department of Medicinal Chemistry houses a 3 channel Agilent DD2 500 MHz spectrometer, which comes equipped with z-axis pulse field gradients (PFGs) and several sets of probes. The instrument provides the most up-to-date multidimensional, multinuclear NMR techniques for studying the structure and dynamics of single biomolecules or biomolecular complexes in solution. Although training in the use of the NMR is not routinely provided to users outside the Department of Medicinal Chemistry, access to the NMR instrument is possible through the Analytical Biopharmacy Core on a fee for service basis. The magnet resides in room I093C of HSB.
Features
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• Agilent DD2 500 MHz instrument
• 1H Frequency: 499.732 MHz
• 2H field-frequency lock system (76.7119 MHz)
• Magnet and Shim system
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o Oxford Standard bore (500/51, 51mm) Cryomagnet
o 28/51/40 shim system with 28 room temperature (RT) shim gradients
o TMC anti-vibration pneumatic system
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• Host Operating System: RedHat Enterprise Linux, Version 6.1
• Variable Temperature (VT) module: achieve stability of ± 0.1°C (the max Temp. range depends on the probehead used)
• RF Section
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o Three RF channels
o Dual (50 W pulsed, 200-500 MHz + 300W pulsed , 6-220 MHz) linear amplifiers
o Dedicated Broadband 300W,linear amplifier for third channel (6-220 MHz)
o Waveform generator (30MB memory) for pulse shaping capability with amplitude and phase modulation. It can create an infinite number of waveforms for experiments.
- o 14 bit digitizer with max oversampling rate of 80 MHz (eff. 20 bit @ 10kHz)
o 100 Hz to 5 MHz spectral width with 0.1 Hz resolution
o Digital dead time of 0.4 μs
- • PFG
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o Performa IV gradient amplifier that can generate a maximum of 70 Gauss/cm Z-axis gradient field strength
o Waveform generator
o Timing resolution of 12.5 ns
o Automated Deuterium Gradient shimming module allows the use of deuterium gradient shimming under automated operation conditions
- o Runs on Vnmrj 3.2
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• 5-mm indirect detection triple resonance 1H{13C/15N} PFG Probe (Z-axis only). Variable temperature range: -20 to +80°C.
• 5-mm direct detection 1H-19F/15N-31P AutoX Dual Broadband PFG Probe (Z-axis only). Variable temperature range: -80 to +130°C
• 5-mm direct detection Broad Band (50-202/500 MHz) probe
For more information about access to this instrument contact the Core manager.
Please acknowledge all work done on core facility instruments by citing the Analytical Biopharmacy Core, its contributing scientists, the Center for Intracellular Delivery of Biologics and the Washington State Life Sciences Discovery Fund. Core personnel directly involved in performing and designing experiments should be considered for authorship.