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The expansion procedure will consist of using fresh or frozen tumor fragments collected from mice with passage 2, 3 or 4 xenografted tumors. The tumor fragments will be implanted into the subcutaneous tissue of the left flank of NSG (NOD scid IL2 receptor gamma chain knockout) mice using a trocar needle and sterile technique.  Depending on the experimental needs of the investigator, mice will be dispensed to the requesting investigator within five days of tumor implantation, or when the first tumor is palpable


Seattle Cancer Consortium Partner Institutions:

Seattle Children's .