Administration of High Fat Diet
The high-fat diet was designed by Richard Surwit to be diabetogenic in C57BL/6 mice. The diet is high in both carbohydrates and fat, consisting of 20% protein, 35.5% lard, 32.7% table sugar, moisture, and fiber. Typical mouse chow is 20% protein, 9% lard, 4% fiber, 6.5% ash, and 2.5% minerals.
Mice will be weighed prior to the study to establish baseline weights, then have their diet switched from standard chow to the SURWIT diet. At every specified timepoint, the mice will be monitored by recording the mouse weight, or any other gross abnormality.