Introduction

Mice can be rederived using in vitro fertilization (IVF). Sperm from a frozen sample or a donor male supplied by the investigator can be used to fertilize embryos from superovulated females. Embryos are incubated overnight to the 2-cell stage, and then surgically re-implanted into pseudopregnant females.

Service Description

• generate pseudopregnant recipient females
• thaw frozen sperm sample or collect fresh sperm from donor male
• surgically implant fertilized embryos into pseudopregnant females
• monitor pregnant females
• wean pups
• The recipients will be tested for pathogens before the pups are transferred to your colony.

Prices and Guarantees

• The cost of IVF for mouse rederivation includes thawing frozen sperm or harvesting sperm from a donor male.
• The investigator is responsible for cage per diem costs.
• If the recipients do not become pregnant for any reason, we will repeat the procedure at no additional cost.
• Some inbred strains do not perform well for IVF.
• Click here for current rates.

Reserve an In Vitro Fertilization Session

To order an IVF session, go to our TRP Forms page. Fill out a “Request for In Vitro Fertilization” form and e-mail it to Bob Hunter bhunter@uw.edu