In Vitro Fertilization (IVF)

Introduction

Mice can be rederived using in vitro fertilization (IVF). Sperm from a frozen sample or a donor male supplied by the investigator can be used to fertilize embryos from superovulated females. Embryos are incubated overnight to the 2-cell stage, and then surgically re-implanted into pseudopregnant females.

Service Description

  • Generate pseudopregnant recipient females.
  • Thaw frozen sperm sample or collect fresh sperm from donor male.
  • Surgically implant fertilized embryos into pseudopregnant females.
  • Monitor pregnant females.
  • Wean pups.
  • The recipients will be tested for pathogens before the pups are transferred to your colony.

Prices and Guarantees

  • The cost of IVF for mouse rederivation includes thawing frozen sperm or harvesting sperm from a donor male.
  • The investigator is responsible for cage per diem costs.
  • If the recipients do not become pregnant for any reason, we will repeat the procedure at no additional cost.
  • Some inbred strains do not perform well for IVF.
  • Click here for current rates.

Reserve an In Vitro Fertilization Session

To order an IVF session, go to our TRP Forms page. Fill out a “Request for In Vitro Fertilization” form and e-mail it to Bob Hunter (bhunter@uw.edu).