Faculty

Sheila A. Lukehart, PhD

• Professor of Medicine and Global Health
• Adjunct Professor of Microbiology
• Director, University of Washington STD & HIV Research Training Program
• Assistant Dean for Research & Graduate Education
• Harborview Medical Center

The Lukehart laboratory studies the pathogenesis of syphilis and the immune response to Treponema pallidum in humans and in animal models. Our current major interest is the 12-membered tpr gene family of T. pallidum, which is hypothesized to encode surface-exposed antigens that are major targets of the protective immune response, may be involved in immune evasion, and are promising vaccine candidates. We have demonstrated that one member of the Tpr family, TprK, undergoes antigenic variation; studies related to the immunological relevance and molecular mechanism of this variation are ongoing.  New studies are .focused on TprC and TprD, which are also surface exposed and which differ in sequence among T. pallidum strains and subspecies. The laboratory is also working to identify surface molecules that are targets of opsonization and to define the kinetics of and requirements for bactericidal activity by macrophages. Many of the projects described above involve collaborations with Drs. Arturo Centurion-Lara and Lorenzo Giacani.

Additionally, our laboratory is involved in studies of clinical aspects of syphilis. With Dr. Christina Marra (Neurology), the laboratory is exploring the molecular basis for neuroinvasion, the immunologic response to T. pallidum within the CNS, and the efficacy of recommended therapy for CNS syphilis in immunocompetent and HIV-infected patients. Other ongoing studies involve the investigation of emerging macrolide resistance, and application of a molecular typing method for T. pallidum to epidemiological studies of syphilis.

Selected Publications

Palmer GH, Bankhead T, Lukehart SA. 'Nothing is permanent but change'- antigenic variation in persistent bacterial pathogens. Cell Microbiol. 11(12):1697-705, 2009.
[ PubMed Abstract ]

Giacani L, Molini BJ, Kim EY, Godornes BC, Leader BT, Tantalo LC, Centurion-Lara A, Lukehart SA. Antigenic variation in Treponema pallidum: TprK sequence diversity accumulates in response to immune pressure during experimental syphilis. J Immunol. 184(7):3822-9. 2010.
[ PubMed Abstract ]

Marra CM, Sahi SK, Tantalo LC, Godornes C, Reid T, Behets F, Rompalo A, Klausner JD, Yin YP, Mulcahy F, Golden MR, Centurion-Lara A, Lukehart SA. Enhanced molecular typing of treponema pallidum: geographical distribution of strain types and association with neurosyphilis. J Infect Dis. ;202(9):1380-8, 2010.
[ PubMed Abstract ]

Ho EL, Lukehart SA. Syphilis: using modern approaches to understand an old disease. J Clin Invest. 121:4584-92, 2011.
[ PubMed Abstract ]

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