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Directory >> Dorothy Patton, PhD

Faculty

Dorothy Patton, PhD

  • Professor, Department of Obstetrics and Gynecology
  • University of Washington

The Patton Lab utilizes several macaque models to study the pathogenesis, treatment and prevention of chlamydial infections. First, the role of the topical microbicides in prevention of sexual transmission of Chlamydia is investigated in both vaginal or rectal flora, changes in the vaginal, cervical, and rectal epithelial tissues, and efficacy in preventing chlamydial infection, both cervical and rectal. Recently, efficacy in preventing Trichomonas vaginalis has been added to the vaginal model. The development of a microbicidal gel which women could use intravaginally or rectally would provide a much needed female-controlled means of STI prevention. We have also initiated studies examining the effects of post coital events and their effects on the cervicovaginal environment. Next, we will assess the effects of topical microbicide use with coital activity.

Second, the macaque model of pelvic inflammatory disease (PID) models upper genital tract disease resulting from ascending cervical infection. This model has been used to evaluate antibiotic and anti-inflammatory treatments for existing chlamydial disease, the immunologic responses to chlamydia, and genetic predisposition to PID. The model is also evaluating safety, immunogenicity and protective efficacy of a number of C. trachomatis vaccine candidates. A new focus of this model is to investigate immunologic factors including the role of Toll-like receptors, which may contribute to ascending versus local cervical chlamydial genital tract infection.

Third, the salpingeal auto transplant ("pocket") model is being used to evaluate the role of various chlamydial antigens and of host immune factors (cytokines) in evoking the typical delayed-type hypersensitivity (DTH) response seen n PID. In collaborations with Dr. W. Van Voorhis and Dr. W. Stamm, we have shown that chlamydial heat shock protein 60 (cHSP60) is a major factor eliciting the DTH response in salpingeal tissues. This finding suggests that the immunologic response seen in PID, a TH1 cytokine pattern, may be one of the host factors that exacerbates the disease by encouraging production of cHSP60. In collaboration with Dr. Patricia Totten, the autotransplant model is also being used to investigate the pathogenesis of Mycoplasma genitalium infection. All studies in the research program are aimed at improving women's reproductive health care, and ultimately eradicating chlamydial infection.


Selected Publications


Coleman J, Hitti J, Coombs R, Lockhart D, Mwachari C, Bukusi E, Gausman R, Jensen S, Patton D, Cohen C. Infectious correlates of HIV-1 shedding in the female upper and lower genital tracts. AIDS. 2007; 21(6): 755-9.
PubMed Abstract


Patton DL, Cosgrove Sweeney YT, Balkus JE, Rohan LC, Moncla BJ, Parniak MA, Hillier SL. Preclinical safety assessments of UC781 anti-HIV topical microbicide formulations. Antimicrob Agents Chemother. 2007; 51(5): 1608-15.
PubMed Abstract


Vincent KL, Bell BA, Rosenthal SL, Stanberry LR, Bourne N, Cosgrove-Sweeney YT, Patton DL, Montamedi M. Application of optical coherence tomography for monitoring changes in cervicovaginal epithelial morphology in macaques: potential for assessment of microbicide safety. Sex Trans Dis. 2008; 35(3): 269-75.
PubMed Abstract


Patton DL, Cosgrove Sweeney YT, Paul KJ. A summary of preclinical topical microbicide vaginal safety and Chlamydial efficacy evaluations in a pigtailed Macaque model. Sex Trans Dis. 2008; 35(10): 889-97.
PubMed Abstract


Patton DL, Cosgrove Sweeney YT, Paul KJ. A summary of preclinical topical microbicide rectal safety and efficacy evaluations in a pigtailed macaque model. Sex Trans Dis. 2009; 36(6): 350-6.
PubMed Abstract


Miyairi I, Ramsey KH, Patton DL. Duration of untreated Chlamydia infection and factors associated with clearance: Review of animal studies. J Infect Dis.2010; 201(S2): S96-103.
• PubMed Abstract

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