CMIC Services

Tissue fixation and processing for light microscopy: Preparation of fixative solutions, performing vascular perfusions, collecting tissues, and freezing tissues.
Embedding and sectioning: Paraffin embedding of fixed tissues and embedding in OTC compound for cryostat sectioning. Paraffin sectioning as well as cryostat sectioning of frozen tissues. Sections are mounted on slides for staining.
Histological and histochemical staining: Routine methods such as hematoxylin and eosin, and specialized histochemical staining techniques such as thioflavin S and Congo red for islet Amyloid, Oil red O for adipocytes, ß-galactosidase for lacZ reporter gene expression, and TUNEL for apoptosis.
Immunocytochemical staining: Peroxidase and fluorescent-labeled antibodies and related methods, including multiple labeling applications.
In situ hybridization: mRNA localization using isotopic (film and emulsion autoradiography) and non-isotopic methods and with labeled riboprobes and oligonucleotides, including multiple ISH labeling.
Flow cytometry: The core oversees the operation and maintenance of flow cytometry instruments for DERC investigators and trains users.
Digital image acquisition: Images of cellular and histological preparations by various microscopic optical modalities including confocal microscopy.
Technical assistance: Personnel to perform services and train users in protocols and use of analytical equipment for cellular and molecular imaging.
Consultation: Consulting assistance with protocols and procedures, as well as with design of their studies and interpretation of results. Consultation is a major function of the core and involves collaborative interactions of affiliates with the core directors.