CFAC Services and Facilities

Flow Culture System

This system is optimal for real time measurements of cellular function. It is currently optimized for pancreatic islets, retinal cells, stem cells, macrophages, adipocytes, endothelial cells, neurons/brain slices and hepatocytes

The following measures can be made at the single cell level:

  • Calcium
  • NAD(P)H
  • Mitochondrial Membrane Potential
  • Reactive Oxygen Species
  • Insulin (Zn++)
  • Oxygen
  • Potassium
  • Protein Kinase A activity
  • Protein Kinase C activity

 

The following measurements can be made in bulk:

  • Insulin secretion
  • Oxygen consumption
  • Cytochrome C
  • Lactate production

 

Static Culture System

This system is preferable where a large number of conditions need to be analyzed in parallel, or measures need to be made in the absence of flow.

This system is also optimized for pancreatic islets, retinal cells, stem cells, macrophages, adipocytes, endothelial cells, neurons and hepatocytes

The following measurements can be made:

  • Insulin secretion and content
  • Ion transport (e.g. K+ and Ca++)
  • Glycolysis and CO2 production
  • Lactate production

 

In addition, assessment of metabolite levels, binding studies and enzyme activity assays are also offered.

Pancreatic Islets

The CFAC can provide investigators with isolated islets from rodents. The core has experience isolating islets from Fischer, Sprague-Dawley, Wistar and BB rat islets, and C57BL/6 mouse islets. Other strains have also been successfully used. Although it is strain-dependent, rodents yield about 700 islets/rat, and 200 islets/mouse. Once prepared, islets may be cultured under desired conditions or treatments until being either shipped or further analyzed by methods listed above.  The Core can also assist investigators with acquiring monkey islets from the Oregon National Primate Research Center and culturing the islets upon arrival.

Dispersed islet cells can also be generated for subsequent culture, analysis or flow cytometry via the Immunology and Inflammation and Core.

Consultation and Training

Input on study design and data analysis together with training in the techniques offered through the core are offered by the core. Contact the CFAC director, Dr. Ian Sweet, for more details.

If the DRC has supported your research in any way, please support us by citing the DRC grant number P30DK017047 in the acknowledgment section of your publications.

DRC Administrative Office, University of Washington Box 358285 | Phone: 206 764-2688 | FAX: 206 764-2693 | DERC@u.washington.edu

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