CMIC Services and Facilities
The CMIC has two main locations, at the VA Puget Sound Health Care System and in the Histology and Imaging Core at UW SLU. These two laboratories provide state-of-the art facilities to DRC Affiliates and enable the following services to be offered:
Tissue Processing and Embedding
Preparation of fixative solutions and tissue processing. Embedding of tissue for paraffin and cryostat sectioning, as well as mounting of sections on slides for subsequent staining.
Immunocytochemical Staining: The CMIC provides peroxidase and fluorescent-labeled antibodies and related methods, including multiple labeling applications. We offer automated immunohistochemistry using the Leica Autostainer.
Histochemical Stains: Routine hematoxylin and eosin (H&E) as well as specialized staining techniques such as thioflavin S and Congo red for islet amyloid, Oil red O for lipid staining, Movat’s pentachrome for extracellular matrix, ß-galactosidase for lacZ reporter gene expression and TUNEL for apoptosis.
Conventional Bright Field and Fluorescence Microscopy
The CMIC facilities at the VA and SLU have a wide variety of upright and inverted Nikon, Leica and Olympus microscopes. Contact Core Directors for more information.
An automated whole slide scanner (Olympus NanoZoomer Digital Pathology Virtual Microscope) scans and digitizes tissue sections.
The digital images are high resolution and preserve information from the original glass slide. Images can be visualized over a network or the internet.
A Leica Inverted SP5 II Advanced Confocal microscope system is available at the Core at VAPSHCS for use by all DRC affiliate investigators.
Live Cell and Wide Field Imaging
The DeltaVision imaging microscope may be used for live-cell imaging and 3D imaging of fluorescent probes It is also useful for fluorescence immunohistochemistry for weak signals that are difficult to image on a conventional confocal microscope.
Laser Capture Microdissection:
The CMIC offers DRC affiliate investigators a Leica LMD7000, housed in the CMIC facility at VAPSHCS.
The CMIC offers transmission electron microscopy through Dr. Alpers and the UW Department of Pathology Electron Microscopy facility.
The CMIC directorship has considerable experience with digital image acquisition and performing semi-quantitative and quantitative morphometry. The core laboratories are all equipped with a variety of imaging programs including Visiopharm Imaging Software, Image ProPlus Software, Nikon Image Analysis Software (NIS Elements), NIH ImageJ Software and Adobe Photoshop.
Visiopharm software is available at the CMIC SLU facility. This allows affiliates to perform automated image analysis on their digital images scanned on the Olympus Nanozoomer. Several protocols have already been developed by CMIC staff. The integration of a whole slide scanner with automated image analysis software greatly improves the speed of morphological data collection and analysis.
Core directors and staff provide consulting services on protocols needed to meet the scientific objectives of affiliate investigators that use the core facilities, as well on procedures for analyzing and interpreting the results of these methods.
A major part of the CMIC’s mission is training of students, fellows and laboratory staff in the techniques offered by the Core. Hands on training is offered at both CMIC laboratories, with workshops and didactic training opportunities occurring frequently.
A unique strength of the CMIC is access to veterinarians who have expertise in comparative pathology. Please contact Dr. Frevert for more information.