HSC Services and Facilities
Hyperinsulinemic-euglycemic clamps can be performed with or without tracers by a specially trained registered nurse practitioner for measurement of hepatic, muscle and adipose tissue insulin sensitivity. Training in these techniques can also be provided upon request.
Hyperglycemic clamps with or without arginine and stepped hyperglycemic clamps are provided by the HSC to measure insulin secretion and beta-cell function.
The HSC also can provide training in or performance of oral glucose or meal tolerance tests and intravenous glucose tolerance tests.
Data from frequently sampled intravenous glucose tolerance tests (FSIGT) can be modeled using MinMod, developed by Bergman et al (Pacini G et al, Computer methods and programs in biomedicine 1986;23:113-122). This provides measures of insulin sensitivity, insulin release and glucose disposal.
Oral glucose and meal tolerance data will be modeled using SAAM II software (Cobelli C, Foster DM, Adv Exptl Med Biol 1998;445:79-101) based on the model developed by Cobelli et al (Dalla Man C et al, Diabetes 2005;54:3265-3273). This model provides various measures of the beta cell’s ability to respond to glucose as measures of beta-cell function.
Metabolic phenotyping procedures are conducted at the UW Medical Center (General Clinical Research Center) or at VA Puget Sound Health Care Center (Clinical Research Unit and in space occupied by the Diabetes Research Group).
The HSC can facilitate access and streamline the process for identifying potential study subjects through Electronic Medical Record (EMR) system. The Human Studies Core can review VA EMR to identify potential subjects for recruitment into both observational and clinical trial research to investigators or a study team that includes a VA investigator. EMR data can be accessed for an individual medical center, a VISN, or nationally. The Human Studies Core also has an agreement with the Group Health Research Institute (GHRI) to facilitate access to the approximately 600,000 members who receive care from Group Health Cooperative. The Human Studies Core will assist with facilitating interactions with GHRI investigators and staff to achieve research recruitment and data procurement objectives. The Human Studies Core also maintains information on numbers of persons with diabetes and their salient characteristics on persons receiving care from the University of Washington medical centers and neighborhood clinics, and can provide this information to investigators. Those wishing to recruit subjects or use data from the University of Washington system will be directed to the ITHS EMR patient recruitment service.
Expert assistance with the design and conduct of clinical research, subject recruitment, and statistical analysis.
The HSC provides diabetes focused consultation on research design, methods, and data analysis not easily obtained from elsewhere in the UW research community. This resource takes advantage of the extensive experience of the HSC director and associate directors. See How to Use for more information.
The Japanese American Community Diabetes Study repository has collected extensive information and to date has yielded over 60 scientific publications authored by not only local scientists but also investigators from around the world. It includes extensive phenotyping and stored plasma and white blood cells from a 10 year prospective evaluation of 658 Japanese Americans who resided in King County, Washington State. Measurements were conducted at baseline, 5- and 10-years and include glucose, insulin, and C-peptide concentrations for a 180-minute oral glucose tolerance test; CT-measurements of visceral and multiple subcutaneous fat depots; lipid profile; and a focused physical examination and medical history.