Course Overview: In this class, students will explore the mechanisms and fate of chemical interaction with biological systems. With the information gathered from this class, students will derive an enhanced appreciation for how foreign chemicals, including pharmaceuticals and environmental agents, interact with cellular pathways to effect toxicological insult. Guest lecturers will provide valuable coverage of subject areas within their respective areas of expertise.

ENVH 514 is the first quarter of a 3-quarter course series. Together, the 3-quarter sequence integrates core subject areas pertaining to environmental and occupational toxicology. In ENVH 514 we will discusses fundamental cellular processes and core areas of toxicology. The second quarter (ENVH 515) focuses primarily on organ system toxicology and the emphasis of the third quarter (ENVH 516) is on specific classes of toxicological agents.

ENVH 514 is organized with lectures pertaining to:

• Dose-response relationships · Genetic toxicology
• Absorption, distribution, metabolism and excretion of toxicants (i.e., toxicokinetics) · Biotransformation

• Signal transduction pathways · Induction & Polymorphism

• Biochemical mechanisms of toxicity · Risk Assessment
• · Biotech/Regulatory Toxicology

· Cell Death & Apoptosis · Toxicogenomics

Intended Student Audience: The ENVH 514/515/516 course sequence serves as the core of the toxicology program for Toxicology graduate students in the Department of Environmental Health. Graduate students from other allied biomedical science departments, e.g., Epidemiology, Medicinal Chemistry, Chemistry, Molecular & Cellular Biology, also participate in the course on a regular basis. Prerequisites for this class include a year of undergraduate general biology, two quarters of organic chemistry, Previous background or concurrent registration in mammalian physiology is strongly recommended.

Required Reading: The text for ENVH 514 is: Casarett and Doull's Toxicology: the Basic Science of Poisons; CD Klaassen, ed.; 6th Edition, and is available at the Health Sciences Branch of the University Bookstore. The same text is also used in the 515 and 516 sections of this course.

Additional handout materials will be provided for most classes and are required reading.

Discussion Groups: At selected points in the quarter, an optional discussion section will meet on selected Thursdays, from 3:30-4:20 PM, in R212. Julia Gholke will direct the discussion groups with assistance from class lecturers. These sessions will provide an opportunity for review of course materials prior to exams and other extended discussion of course subject matter.

Grading: The final grade for this class will be compiled from equally-weighted (?) scores from a midterm and a final exam plus 1 group presentation.

Group Presentation: Groups of three or four students will choose projects on basic environmental health issues for independent research and presentation to the class. Sample issues include global climate change, heavy metal exposure and toxicity, , and solid waste treatment methods. Each group is expected to outline the problems and discuss current and future methods of treating the problems, in 15-minute presentations and posters, presented at the last class.

09/30/02 Course Overview / Kavanagh Chapter 1

Dose-Response Chapter 2

10/02/02 Absorption Pierce Chapter 5

10/04/02 Distribution Pierce Chapter 6

10/07/02 Biotransformation I Pierce Chapter 6

10/09/02 Biotransformation II Pierce Chapter 6

10/11/02 Excretion Pierce Chapter 6

10/14/02 Toxicokinetics I Pierce Chapter 7

10/16/02 Toxicokinetics II Pierce Chapter 7

10/18/02 Toxicokinetics III Pierce Chapter 7

10/21/02 Biochemical Mechanisms of Kavanagh Chapter 3

Toxicity I (pp. 40-48)

10/23/02 Biochemical Mechanisms of Kavanagh Chapter 3

Toxicity II (pp. 57-61; 64-66)

10/24/02 Discussion/Review Gohlke

Roosevelt R212

10/25/02 In-Class Exam I

(Covers 09/30-10/23)

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10/28/02 Signal Transduction Franklin Chapter 3

Mechanisms I (pp. 48-57)

10/30/02 Signal Transduction Franklin Handouts

Mechanisms II

11/01/02 Cell Death & Apoptosis I Franklin Chapter 3

(pp. 61-64)

11/04/02 Cell Death & Apoptosis II Franklin Handouts

11/06/02 Carcinogenesis I Kavanagh Chapter 8

11/08/02 Carcinogenesis II Kavanagh Chapter 8

11/11/02 VETERANS DAY HOLIDAY - NO CLASS

11/13/02 Genetic Toxicology I Kavanagh Chapter 9

11/15/02 Genetic Toxicology II Kavanagh Chapter 9

11/18/02 Gene Induction Kavanagh Handouts

11/20/02 Toxicogenomics I Bammler Handouts

11/22/02 Ecogenetics/Env Epidemiology Eaton Handouts

11/25/02 Proteomics Farin/Sidhu? Handouts

11/27/02 Metabonomics Kavanagh Handouts

11/29/02 THANKSGIVING HOLIDAY - NO CLASS

12/02/02 Risk Assessment I Pierce Chapter 4

Handouts

12/04/02 Risk Assessment II Pierce Chapter 4

Handouts

12/06/01 Biotech/Regulatory Toxicology I Ponce/Bussiere Chapter 34

12/09/01 Biotech/Regulatory Toxicology II Ponce/Bussiere Chapter 34

12/11/02 Group Presentations Gohlke

12/17/02 Final Examination Kavanagh/Pierce

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