Our Research: Faculty/Research Laboratories

Christiane Hampe, PhD

Research Associate Professor, Department of Medicine, Division of Metabolism, Endocrinology and Nutrition

Email Address: champe@u.washington.edu

Division of Metabolism, Endocrinology and Nutrition Website:  http://depts.washington.edu/metab/

Background:

Chris Hampe received her PhD at the Weizmann Institute of Science in Israel in 1997. She did her post-doctoral training in Dr. Åke Lernmark’s laboratory at the University of Washington, Seattle and is currently a Research Associate Professor at the Diabetes and Obesity Center of Excellence at the University of Washington.

Focus:

Dr. Hampe’s research interests center on human autoimmune diseases and the role of autoantibodies in disease pathogenesis with particular interest in Type 1 diabetes and Stiff Person Syndrome. More specifically, areas of focus are as follows.

The role of autoantibodies to GAD65 (GAD65Ab) in the pathogenesis of type 1 diabetes.

In marked contrast to the traditional view, our recent studies and preliminary results suggest that GAD65Ab are common in healthy individuals, but masked by epitope-specific anti-idiotypic antibodies.

Isolation and characterization of b96.11-specific B lymphocytes from human periperhal blood.

In collaboration with Dr. BenHar in Israel, we cloned and expressed five human monoclonal anti-idiptypic antibodies that bind specifically to b96.11. The antibodies will be used to specifically deplete b96.11-specific lymphocytes to interfere with the autoimmune pathogenesis leading to development of T1D.

Specific depletion of GAD65-specific B lymphocytes in NOD mice.

Injection with GAD65Ab-specific anti-Id-8E6G4 reduced the number of GAD65-specific B plasma cells by 85%. Investigation of GAD65-specific T cell responses in NOD mice injected with the above antibody are currently underway in collaboration with Dr. John Gebe, BRI.

GAD65Ab in Stiff Person Syndrome

GAD65Ab may have a pathogenic role in the disease process in patients with Stiff Person Syndrome (SPS). GAD65Ab in SPS patients inhibit the enzyme’s activity and result in a deficit in the major inhibitory neurotransmitter GABA, possibly causing clinical symptoms such as muscle spasms and stiffness. Often, the diagnosis of SPS is delayed due to its relative rareness and patients experience dilapidating clinical symptoms. Muscle stiffness and pain are common complaints in the general population. Often no physical cause can be found and appropriate treatment cannot be recommended. We hypothesize that autoimmunity to GAD65 is present in a sizeable portion of these cases.

In vivo administration of GAD65Ab into rat cerebellum

In collaboration with Drs. Honnorat and Manto in France and Belgium, we established that GAD65Ab play an active role in the pathogenesis of SPS. Such a role had been hypothesized based on the observation that SPS patients have high GAD65Ab titers which inhibit GAD65 enzyme activity and show decreased GABA concentrations. The mechanism of this pathogenesis remained however unclear. We continued our collaboration and investigation of the potential role of GAD65Ab in SPS. We found that GAD65Ab are internalized into a rat neuronal cell line. GAD65Ab injections induced behavioral changes in rats.

Representative Publications:

Ortqvist E, Brooks-Worrell B, Lynch K, Radtke J, Bekris LM, Kockum I, Agardh CD, Cilio CM, Lethagen AL, Persson B, Lernmark A, Reichow J, Oak S, Palmer JP, Hampe CS. Changes in GAD65Ab-specific anti-idiotypic antibody levels correlate with changes in C-peptide levels and progression to islet cell autoimmunity. J Clin Endocrinol Metab. 95(11):E310-318, 2010. PMCID: PMC2968730

Oak S, Gaur LK, Radtke J, Patel R, Iyer D, Ram N, Gaba R, Balasubramanyam A, Hampe CS Masked and Overt Autoantibodies Specific to the DPD Epitope of 65-kDa Glutamate Decarboxylase (GAD65-DPD) Are Associated With Preserved β-Cell Functional Reserve in Ketosis-Prone Diabetes J Clin Endocrinol Metab. 2014 Jun;99(6):E1040-4. doi: 10.1210/jc.2013-4189. PMCID: PMC4037721

Larsson HE, Jönsson I, Lernmark Å, Ivarsson S, Radtke JR, Hampe CS and the Diabetes Prediction in Skåne (DiPiS) and TrialNet studies. Decline in titers of anti-Idiotypic antibodies specific to the b96.11 GAD65-specific monoclonal antibody precedes development of GAD65 autoantibodies and type 1 diabetes. PLoS One. 2013 Jun 13;8(6):e65173.

Oak S, Gilliam LK, Landin-Olsson M, Törn C, Kockum I, Pennington CR, Rowley MJ, Christie MR, Banga JP, Hampe CS. The lack of anti-idiotypic antibodies, not the presence of the corresponding autoantibodies to glutamate decarboxylase, defines type 1 diabetes. Proc Natl Acad Sci U S A. 2008 105:5471-6 PMCID: PMC2291139

Hampe CS, Petrosini L, De Bartolo P, Caporali P, Cutuli D, Laricchiuta D, Foti F, Radtke JR, Vidova V, Honnorat J, Manto M Monoclonal antibodies to 65kDa glutamate decarboxylase induce epitope specific effects on motor and cognitive functions in rats. Orphanet J Rare Dis. 2013 Jun 5;8:82.

 

  • Kopelowitz J, Hampe C, Goldman R, Reches M, Engelberg-Kulka H. Influence of codon context on UGA suppression and readthrough. J Mol Biol 225: 261-9, 1992.

    Hampe CS, Pecht I. Protein tyrosine phosphatase activity enhancement is induced upon Fc epsilon receptor activation of mast cells. FEBS Lett. 346: 194-8, 1994.

    Hampe CS, Pecht I. Purification and preliminary characterization of an Fcc-receptor-activated protein tyrosine phosphatase from mast cells. Euro J Biochem, 251: 964-70. 1998.

    Hampe CS, Örtqvist E, Rolandsson O, Landin-Olsson M, Törn C, Hallmans G, Persson B, Schranz DB, Lernmark Å. GAD65 autoantibodies in IDDM patients are specific for different GAD65 species. J. Clin. Endocrinol. Metab. 84: 643-8 1999.

    Hampe CS, Örtqvist E, Persson B, Schranz DB, Lernmark Å. Glutamate decarboxylase (GAD) autoantibody epitope shift during the first year of type 1 diabetes. Horm Metab Res. 31:553-7, 1999.

    Bieg. S., Hanlon, C., Hampe, CS., Benjamin, D., Mahoney, CP. GAD65 and Insulin B Chain Peptide (9-23) are not primary Autoantigens in the Type 1 Diabetes Syndrome of the BB Rat. Autoimmunity 31:15-24, 1999.

    Rolandsson O, Hagg E, Hampe CS., Sullivan EP, Nilsson M, Hallmans G, Lernmark Å. Glutamate decarboxylase (GAD65) and tyrosine phosphatase-like protein (IA-2) autoantibodies index in a regional population is related to glucose intolerance and body mass index. Diabetologia; 42:555-9, 1999.

    Hao W, Davis C, Daniels T, Hampe CS., Lernmark Å. Epitope-specific glutamic acid decarboxylase-65 autoantibodies in intravenous immunoglobulin preparations. Transfus Med 9: 307-310, 1999.

    Hampe CS, Hammerle LP, Bekris L, Örtqvist E, Kockum I, Rolandsson O, Landin-Olsson M, Törn C, Persson B, Lernmark Å. Recognition of glutamic acid decarboxylase (GAD) by autoantibodies from different GAD antibody-positive phenotype. J. Clin. Endocrinol. Metab. 85: 4671-9, 2000.

    Novak EJ, Örtqvist E, Nord E, Edwall L, Hampe CS, Bekris L, Persson BE, Lernmark Å. Stability of disease-associated antibody titers in pregnant women with type 1 diabetes with or without residual beta-cell function. Diabetes Care 23: 1019, 2000.

    Philosof-Oppenheimer R, Hampe CS, Schlessinger K, Fridkin, M and Pecht I. An immunoreceptor tyrosine-based inhibitory motif, with serine at site Y-2, binds SH-2-domain-containing phosphatases. Eur J Biochem 267: 703-711, 2000.

    Hampe CS, Lundgren P, Daniels TL, Hammerle LP, Marcovina SM, and Lernmark Å. A Novel Monoclonal Antibody Specific for the N-Terminal End of GAD65. J Neuroimmunol. 113:63-71, 2001.

    Hampe CS, Hammerle LP, Falorni A, Robertson J and Lernmark Å. Site-Directed Mutagenesis of K396R of the 65 kDa Glutamic Acid Decarboxylase active Site Inhibits Enzyme Activity but not Antibody Binding. FEBS Lett. 488:185-9, 2001.

    Plesner A, Hampe CS, Daniels TL, Hammerle LP, Lernmark Å. Preservation of Enzyme Activity and Antigenicity After Mutagenesis of the Membrane Anchoring Domain of GAD65. Autoimmunity, 34: 221-230, 2001.

    Hampe CS, Hammerle LP, Bekris L, Örtqvist E, Persson B, and Lernmark Å. Stable GAD65 Autoantibody Epitope Patterns in Type 1 Diabetes Children Five Years after Onset. J Autoimmun. 18: 49-53, 2002.

    Hampe CS, Kockum I, Landin-Olsson M, Törn C, Örtqvist E, Persson B, Rolandsson O, Palmer JP, and Lernmark Å. GAD65 antibody epitope patterns of patients with Type 1.5 differ from that of Type 1 diabetes patients. Diabetes Care 25:1481-1482, 2002.

    Chessler SD; Hampe CS; Örtqvist E; Persson P; Bekris L; Lernmark Å. Role of the Islet Variant of GAD67, GAD25, as an Autoantigen in Type 1 Diabetes Mellitus. Autoimmunity 35: 335-341, 2002.

    Niklasson B; Heller KE; Schonecker B; Bildsoe M; Daniels T; Hampe CS; Widlund P; Simonson WT; Schaefer JB; Rutledge E; Bekris L; Lindberg AM; Johansson S; Ortqvist E; Persson B; and Lernmark A. Development of Type 1 Diabetes in Wild Bank Voles Associated With Islet Autoantibodies and the Novel Ljungan Virus. Int. Jnl. Experimental Diab. Res,. 4:1-10, 2003. PMCID: PMC2480497

    Elfvin AM, Lindberg BA, M. Landin-Olsson M, Hampe CS, Lernmark A, Ivarsson SA Islet Cell Autoantibodies in Cord Blood from Children with Blood Group Incompatibility or Hyperbilirubinemia Autoimmunity, 36:111-115, 2003.

    Maldonado M, Hampe CS, Iyer D, Gaur LK, Hammerle LP, Bolgiano D, Rodriguez L, Rajan A, Lernmark Å, Balasubramanyam Ketosis-prone diabetes: dissection of a heterogeneous syndrome using an immunogenetic and beta-cell functional classification, prospective analysis, and clinical outcomes. J Clin Endocrinol Metab., 88:5090-8, 2003.

    Luo D, Rogers CN, Hampe CS, Lernmark Å. Molecular engineering of biotin-glutamic acid decarboxylase 65 fusion protein (Biotin-GAD65) for non-radioactive GAD65 antibody assay. J Biotechnol., 103:249-55, 2003.

    Padoa CJ, Banga JP, Madec AM, Ziegler M, Schlosser M, Oertqvist E, Kockum I, Palmer J, Rolandsson O, Foote J, Hampe CS. Recombinant Fab of human mAbs specific to the middle epitope of GAD65 inhibit type 1 diabetes-specific GAD65Ab. Diabetes, 52:2689-95, 2003.

    Luo, D., Rogers, CN, Steed JT, Hampe CS, Lernmark, A Development of glutamic acid decarboxylase 65 (GAD65) autoantibody assay using biotin-GAD65 fusion protein. J. Biotechnol. 111: 97-104, 2004.

    Gilliam LK, Binder K.A, Ortqvist E, Banga J.P, Madec A.M, Luo D, Hampe C.S. Multiplicity of the Antibody Response to GAD65 in Type 1 Diabetes Clin Exp Immunol 138:337–341, 2004 PMCID: PMC1809224

    Luo D, Schranz DB, Bekris L, Hampe CS, Daniel TL, Richter W, and Lernmark Å. A GAD65-E517P Mutant Identifies Conformation-dependent Autoantibodies As Risk Marker for Type 1 Diabetes. Diabetologia, 47: 1581-1591, 2004

    Maioli M, Alejandro E, Tonolo G, Gilliam LK, Bekris L, Hampe CS, Obinu DA, Manconi A, Puddu L, Lynch K, Lernmark A; Study Group for the Genetics of Diabetes in Sardinia. Epitope-restricted 65-kilodalton glutamic acid decarboxylase autoantibodies among new-onset Sardinian type 2 diabetes patients define phenotypes of autoimmune diabetes. JCEM. 89:5675-82 2004.

    Binder K.A., Banga J.P., Madec A-M, Ortqvist E, Luo D, Hampe C.S. Epitope analysis of GAD65Ab using fusion proteins and rFab. J of Immunol Meth 295: 101-109 2004.

    Schlosser M, Banga JP, Madec AM, Binder KA, Strebelow M, Rjasanowski I, Wassmuth R, Gilliam LK, Luo D, Hampe CS Dynamic Changes of GAD65Ab Epitope Specificities during Progression to Type 1 Diabetes. Diabetologia. 48: 922-30 2005.

    Padoa CJ, Crowther NJ, Thomas JW, Lernmark A, Ortqvist E, Palmer JP, Luo D, Hampe CS Epitope mapping of Insulin Autoantibodies using recombinant Fab. Clin Exp Immunol. 140: 564-71 2005. PMCID: PMC1809383

    Hampe CS, Wallen A, Binder KA, Schlosser M, Ziegler M, Sweet IR Quantitative evaluation of a monoclonal antibody and its fragment as potential markers for pancreatic beta cell mass. Exp Clin Endocrinol Diabetes. 113: 381-7 2005.

    Waldrop MA, Sucko AT, Hall TR, Hampe CS, Marcovina SM, Chessler SD A highly sensitive immunoassay resistant to autoantibody interference for detection of the diabetes-associated autoantigen GAD65 in blood and other biological samples. Diabetes Technol Ther. 2:207-18. 2006

    Raju R, Foote J, Banga JP, Hall TR, Padoa CJ, Dalakas MC, Ortqvist E, Hampe CS Analysis of GAD65 Autoantibodies in Stiff Person Syndrome Patients. J Immunol. 2005 175:7755-62 2005

    Palmer JP, Hampe CS, Chiu H, Goel A, Brooks-Worrell BM Is LADA Distinct from T1DM or Just T1DM at an Older Age? Diabetes 54(suppl):S62-7 2005

    Pihoker C, Gilliam LK, Hampe CS, Lernmark A. Autoantibodies in Diabetes Diabetes 54(suppl):S52-61 2005

    Balasubramanyam A, Garza G, Rodriguez L, Hampe CS, Gaur L, Lernmark A, Maldonado MR. Accuracy and predictive value of classification schemes for ketosis-prone diabetes. Diabetes Care. 29:2575-9, 2006

    Hall TR, Thomas JW, Padoa CJ, Torn C, Landin-Olsson M, Ortqvist E, Hampe CS. Longitudinal epitope analysis of insulin-binding antibodies in type 1 diabetes. Clin Exp Immunol. 146:9-14, 2006 PMCID: PMC1809737

    Hampe CS, Nalini R, Maldonado MR, Hall TR, Garza G, Iyer D, Balasubramanyam A. Association of amino-terminal-specific anti-glutamate decarboxylase (GAD65) autoantibodies with beta cell functional reserve and a milder clinical phenotype in patients with GAD65 antibodies and ketosis prone diabetes mellitus. J Clin Endocrinol Metab. 92:462-7, 2007

    Fenalti G, Hampe CS, O’connor K, Banga JP, Mackay IR, Rowley MJ, El-Kabbani O. Molecular characterization of a disease associated conformational epitope on GAD65 recognised by a human monoclonal antibody b96.11. Mol Immunol. 44:1178-89, 2007

    Rui M, Hampe CS, Wang C, Ling Z, Gorus FK, Lernmark A, Pipeleers DG, De Pauw PE. Species and epitope specificity of two 65 kDa glutamate decarboxylase time-resolved fluorometric immunoassays. J Immunol Methods. 319:1-2, 133-143, 2007

    Hoeldtke RD, Hampe CS, Bekris LM, Hobbs G, Bryner KD, Lernmark A, Antibodies to GAD65 and Peripheral Nerve Function in the DCCT. J Neuroimmunol. 185:182-9 2007

    Hampe, CS. Hall, TR, Agren, A., Rolandsson O. Longitudinal changes in Epitope recognition of Autoantibodies against Glutamate decarboxylase 65 (GAD65Ab) in Prediabetic Adults Developing Diabetes. Clin Exp Immunol. 148:72-8 2007 PMCID: PMC1868852

    Bekris, LM, Jensen, RA, Lagerquist, E, Hall, TR, Agardh CD, Cilio CM, Lethagen AL, Lernmark, A, Robertson RA, Hampe CS. GAD65 autoantibody epitopes in adult patients with latent autoimmune diabetes following GAD65 vaccination. Diabet Med. 24:521-6 2007

    Fenalti G, Law RHP, Buckle AM, Langendorf C, Tuck K, Rosado CJ, Faux NG, Mahmood K, Hampe CS, Banga JP, Wilce M, Schmidberger J, Rossjohn J, El-Kabbani O, Pike RN, Smith AI, Mackay IR, Rowley MJ, Whisstock JC GABA production by glutamic acid decarboxylase is regulated by a dynamic catalytic loop. Nat Struct Mol Biol. 14:280-6 2007

    Elfving M, Lindberg B, Lynch K, Ivarsson SA, Lernmark A, Hampe CS Anti-GAD65 immunoglobulin epitopes at birth differ from both the mother’s and serum taken at diagnosis of Type I diabetes Horm Metab Res. 2007; 39:790-6

    Lewy H, Hampe CS, Kordonouri O, Haberland H, Landin-Olsson M, Torn C, Laron Z. Seasonality of Month of Birth differs between Type 1 Diabetes patients with pronounced beta cell autoimmunity and individuals with lesser or absent beta cell autoimmunity Pediatr Diabetes. 2008 9(1):46-52.

    Hall T R, Bogdani M, LeBoeuf R, Kirk, E, Maziarz M, Banga J P, Oak, S, Pennington C, Hampe CS Modulation of diabetes in NOD mice by GAD65-specific monoclonal antibodies is epitope specific and accompanied by anti-idiotypic antibodies. Immunology. 2008 ;123(4):547-54.

    Maruyama T, Oak S, Hall TR, Banga JP, Ortqvist E, Ettinger RA, Endl J, Hampe CS Autoantibody epitopes to the smaller isoform of glutamate decarboxylase do not differ in Swedish and Japanese type 1 diabetes patients and may be associated with high risk HLA class II alleles. Clin Exp Immunol. 2007 150(3):416-21

    Fenalti G, Hampe CS, Arafat Y, Law RH, Banga JP, Mackay IR, Whisstock JC, Buckle AM, Rowley MJ. C-terminal clustering of autoantibody and T cell determinants on the structure of GAD65 provide insights into the molecular basis of autoreactivity. Diabetes. 2008 57:1293-301

    Balasubramanyam A, Nalini R, Hampe CS, Maldonado M. Syndromes of Ketosis-Prone Diabetes Mellitus. Endocr Rev. 2008 29:292-302

    Raju R, Hampe CS. Immunobiology of stiff-person syndrome. Int Rev Immunol. 2008;27:79-92.

    Nalini R, Gaur LK, Maldonado M, Hampe CS, Rodriguez L, Garza G, Lernmark A, Balasubramanyam A. HLA class II alleles specify phenotypes of Ketosis-Prone Diabetes (KPD). Diabetes Care. 2008;31(6):1195-200.

    Steed J, Gilliam LK, Harris RA, Lernmark A, Hampe CS. Antigen presentation of detergent-free glutamate decarboxylase (GAD65) is affected by human serum albumin as carrier protein. J Immunol Methods. 2008 334:114-21

    Oak S, Gilliam LK, Landin-Olsson M, Törn C, Kockum I, Pennington CR, Rowley MJ, Christie MR, Banga JP, Hampe CS. The lack of anti-idiotypic antibodies, not the presence of the corresponding autoantibodies to glutamate decarboxylase, defines type 1 diabetes. Proc Natl Acad Sci U S A. 2008 105:5471-6.

    Maruyama T, Oak S, Shimada A, Hampe CS. GAD65 autoantibody responses in Japanese latent autoimmune diabetes in adults (LADA) patients. Diabetes Care. 2008 31:1602-7

    Maruyama T, Koyama A, Hampe CS. Latent autoimmune diabetes in an adult. Ann N Y Acad Sci. 2008 1150:267-9.

    Haaland WC, Scaduto DI, Maldonado MR, Mansouri DL, Nalini R, Iyer D, Patel S, Guthikonda A, Hampe CS, Balasubramanyam A, Metzker ML. The “A-{beta}-” subtype of Ketosis-Prone Diabetes (KPD) is not predominantly a monogenic diabetic syndrome. Diabetes Care. 2009 32:873-7.

    Hampe CS. Engineered antibodies for type 1 diabetes. Curr Opin Investig Drugs. 2009 10:336-45.

    Oak S, Phan TH, Gilliam LK, Hirsch IB, Hampe CS. Animal insulin therapy induces a biased insulin antibody response that persists for years after introduction of human insulin. Acta Diabetol. 2010;47(2):131-5.

    Padoa CJ, Larsen SA, Hampe CS, Gilbert JA, Dagdan E, Hegedus L, Dunn-Walters D, Banga JP. Clonal relationships between TSH-receptor stimulating antibodies illustrate the effect of hypermutation on antibody function Immunology 2010;129(2):300-8.

    Oak, S, Radtke J, Landin-Olsson M, Törn C, Lernmark A, Hampe CS. Comparison of three assays for the detection of GAD65Ab-specific anti-idiotypic antibodies. J Immunol Methods. 2009 351:55-61

    Fariba Vaziri Sani F-V, Oak S, Radtke J, Lernmark A, Lynch K, Agardh CD, Cilio CM, Lethagen AL, Örtqvist E, Landin-Olsson M, Törn C, Hampe CS ZnT8 autoantibody titers in type 1 diabetes patients decline rapidly after clinical onset. 2010 Autoimmunity 43(8):598-606

    Nalini R, Maldonado M Ozer K, Patel SG, Guthikonda A, Villanueva J, Pham VS, Hampe CS, Gaur LK, Balasubramanyam A Presence or absence of a known DKA precipitant defines distinct syndromes of “A-<beta>+” Ketosis-Prone Diabetes (KPD) based on long-term beta cell function, HLA class II alleles, and gender predilection. 2010 Metabolism 59(10):1448-55

    Hänninen A, Soilu-Hänninen M, Hampe CS, Deptula A, Geubtner K, Ilonen J, Knip M, Reijonen H 2010 Characterization of CD4+ T cells specific for glutamic acid decarboxylase (GAD65) and proinsulin in a patient with stiff-person syndrome but without type 1 diabetes Diabetes/Metabolism Research and Reviews 26(4):271-9.

    Chéramy M, Skoglund C, Johansson I, Ludvigsson J, Hampe CS, Casas R. GAD-alum treatment in patients with type 1 diabetes and the subsequent effect on GAD65 enzyme activity, GADA IgG subclass distribution and humoral response. Clin Immunol. 2010 137(1):31-40.

    Rizzi M, Knoth R, Hampe CS, Lorenz P, Gougeon ML, Lemercier B, Venhoff N, Ferrera F, Salzer U, Thiesen HJ, Peter HH, Walker UA, Eibel H. Long-lived plasma cells and memory B cells produce pathogenic anti-GAD65 autoantibodies in Stiff person syndrome. PLoS One. 2010 26;5(5):e10838.

    E. Örtqvist, B. Brooks-Worrell, K. Lynch, J. Radtke, L. M. Bekris, I. Kockum, C.-D. Agardh, C. M. Cilio, A. L. Lethagen, B. Persson, Å. Lernmark, J. Reichow, S. Oak, J. P. Palmer, C. S. Hampe Changes in GAD65Ab-specific anti-idiotypic antibody levels correlate with changes in C-peptide levels and progression to islet cell autoimmunity. 2010 J Clin Endocrinol Metab 2010 95(11):E310-8

    Korff CM, Parvex P, Cimasoni L, Wilhelm-Bals A, Hampe CS, Schwitzgebel VM, Michel M, Siegrist CA, Lalive PH, Seeck M. Encephalitis associated with GAD-autoantibodies in a child: a treatable condition? 2011 Arch Neurol; 68(8):1065-8.

    Manto MU, Hampe CS, Rogemond V, Honnorat J. Respective implications of glutamate decarboxylase antibodies in stiff person syndrome and cerebellar ataxia. Orphanet J Rare Dis. 2011 4;6:3.

    Yarlagadda A, Hampe CS, Clayton AH. The blood brain barrier and the role of ratiometric molecular analysis in schizophrenia. Psychiatry (Edgmont). 2010;7(12):20-3.

    S. Oak, J. Radtke, C. Törn, M. Landin-Olsson, C. S. Hampe Immunoglobulin subclass profiles of anti-idiotypic antibodies to GAD65Ab differ between type 1 diabetes patients and healthy individuals. Scand J Immunol. 2011; 74(4):363-7

    Yarlagaclcla A, Taylor JH Jr, Hampe CS, Alfson E, Clayton AH. GAD65 Antibodies, Chronic Psychosis, and Type 2 Diabetes Mellitus. Innov Clin Neurosci. 2011;8(8):34-36.

    Skoglund C, Chéramy M, Casas R, Ludvigsson J, Hampe CS. GAD autoantibody epitope pattern after GAD-alum treatment in children and adolescents with type 1 diabetes. Pediatr Diabetes. 2011

    Hampe CS Protective Role Of Anti-Idiotypic Antibodies In Autoimmunity – Lessons For Type 1 Diabetes, Autoimmunity. 2012 45(4):320-31.

    Wang X, Zhang A, Liu Y, Yu J, Feng Z, Shang W, Radtke JR, Hampe CS Modulation of Type 1 Diabetes by a Novel Murine Anti-idiotypic Antibody specific to Human Monoclonal GAD65Ab b96.11. PLoS One. 2012;7(2):e32515.

    C.S. Hampe B Cells in Autoimmunity. 2012 Scientifica

    Chéramy M, Hampe CS, Ludvigsson J, Casas R Characteristics of GAD65 autoantibodies (GADA) in high titer positive individuals. Clin Exp Immunol. 2013,171(3):247-54.

    Diabetes Patel S, Hsu J, Jahoor F, Coraza I, Bain J, Stevens R, Iyer D, Nalini R, Ozer K, Hampe CS, Newgard C, Balasubramanyam A Pathogenesis of A-β+ Ketosis-Prone Diabetes. 2013 62(3):912-22.

    C. S. Hampe, M. E. Maitland, L. K. Gilliam, T.-H. Thi Phan, I. R. Sweet, J. R. Radtke, V. Bota, B. R. Ransom, I. B. Hirsch High titers of autoantibodies to glutamate decarboxylase in Type 1 Diabetes Patients: Epitope Analysis and Inhibition of Enzyme Activity. Endocr Pract. 2013,19:1-20.

    Vega-Flores G, Rubio SE, Jurado-Parras MT, Gómez-Climent MA, Hampe CS, Manto M, Soriano E, Pascual M, Gruart A, Delgado-García JM. The GABAergic Septohippocampal Pathway Is Directly Involved in Internal Processes Related to Operant Reward Learning. Cereb Cortex. 2013.

    Fernandez R, Misra R, Nalini R, Hampe CS, Ozer K, Balasubramanyam A. Characteristics of Patients with Ketosis-Prone Diabetes (KPD) Presenting with Acute Pancreatitis: Implications for the Natural History and Etiology of a KPD Subgroup. Endocr Pract. 2013 25:1-24.

    Chantal E Hargreaves, Marco Grasso, Steve Atkinson, Christiane S Hampe, Brendan Wren, Deborah Dunn-Walters, J Paul Banga Antibodies to thyrotropin hormone receptor in Graves’ disease, maturing from non-autoreactive germline precursor genes, display binding to envelope proteins of Yersinia enterocolitica, J Immunol. 2013;190(11):5373-81.

    Brooks-Worrell BM, Iyer D, Coraza I, Hampe CS, Nalini R, Ozer K, Narla R, Palmer JP, Balasubramanyam A. Islet Specific T cell Responses and Pro-inflammatory monocytes define Specific Subtypes of autoantibody-negative Ketosis-prone diabetes (KPD) Diabetes Care. 2013 Dec;36(12):4098-103.

    Hampe CS, Petrosini L, De Bartolo P, Caporali P, Cutuli D, Laricchiuta D, Foti F, Radtke JR, Vidova V, Honnorat J, Manto M. Monoclonal antibodies to 65kDa glutamate decarboxylase induce epitope specific effects on motor and cognitive functions in rats. Orphanet J Rare Dis. 2013 Jun 5;8:82.

    Larsson HE, Jönsson I, Lernmark Å, Ivarsson S, Radtke JR, Hampe CS and the Diabetes Prediction in Skåne (DiPiS) and TrialNet studies. Decline in titers of anti-Idiotypic antibodies specific to the b96.11 GAD65-specific monoclonal antibody precedes development of GAD65 autoantibodies and type 1 diabetes. PloS One 2013;13;8(6)

    Lester Shulman, Christiane S. Hampe, Avi Ben-Haroush, Yuri Perepliotchikov, Fariba Vaziri-Sani, Shoshana Israel, Keren Miller, Bari Kaplan, Zvi Laron Antibodies to Beta Cell Autoantigens, Rotaviruses, and/or Enteroviruses in Cord Blood And Healthy Mothers in Relation to the 2010-2011 Winter Viral Seasons in Israel – A Pilot Study. Diabet Med. 2014

    C. S. Hampe, R.i Patel, R. Nalini, L. Gaur A. Balasubramanyam Masked” and overt autoantibodies specific to the DPD epitope of 65 kDa glutamate decarboxylase (GAD65-DPD) are associated with preserved beta cell functional reserve in Ketosis-Prone Diabetes. JCEM 2014

    High Prevalence of Type 2 Diabetes-Associated TCF7L2 SNP rs7903146 TT Carriers among Single Islet Autoantibody Positive Children with Type 1 Diabetes Maria J. Redondo, Jesse Muniz, Luisa M. Rodriguez, Dinakar Iyer, Fariba Vaziri-Sani, Morey W. Haymond, Christiane S. Hampe, Michael L. Metzker, Ashok Balasubramanyam 2014 (in print)

    Serum Adiposity-Induced Biomarkers in Obese and Lean Children with Recently Diagnosed Autoimmune Type 1 Diabetes Maria J. Redondo, Luisa M. Rodriguez, O’Brian E. Smith, Morey W. Haymond, Christiane S. Hampe, Sridevi Devaraj , Ashok Balasubramanyam Pediatric Diabetes 2014 (in print)

    Rountree AM, Neal AS, Lisowski M, Rizzo N, Radtke J, White S, Luciani DS, Kim F, Hampe CS, Sweet IR. Control Of Insulin Secretion By Cytochrome C and Calcium Signaling In Islets With Impaired Metabolism. Biol Chem. 2014 May 19. pii: jbc.M114.556050. [Epub ahead of print]

Current Collaborations:

Within the Diabetes and Obesity Center of Excellence and its Affiliated Members

Irl Hirsch, MD
Jay Taborsky, PhD
Ian Sweet, PhD
Jerry Palmer, MD
Jerry Nepom, MD, PhD
Barabara Brooks-Worrell, PhD
John Gebe, PhD
William Banks, MD

Outside the Diabetes and Obesity Center of Excellence

John Neumaier MD. PhD, Seattle
Ake Lernmark, MD, PhD, Sweden
Olov Rolandsson MD, PhD, Sweden
Annelie Carlsson, MD, Sweden
Helena Larsson, MD, PhD, Sweden
Eva Ortqvist, MD, PhD, Sweden
Ashok Balasubramanyam, MD, Houston
Maria Redondo, MD, Houston
Paul Banga, PhD, UK
Merrill Rowley, PhD, Australia
Mario Manto, MD, PhD. Belgium
Jerome Honnorat, MD, PhD, France
Hiroshi Mitoma, MD, PhD, Japan
Carl-Henrik Brogren, PhD, Denmark
Zvi Laron, MD, Israel
Itai Benhar, PhD, Israel,
Lester Shulman, PhD, Israel

 

Lab Members:

Jared Radtke, Lab Manager
Xin Wang, Visiting Scholar