DRC Viral Vector and Transgenic Mouse Core

The overall goal of the Viral Vector and Transgenic Mouse Core (VTMC) is to provide Diabetes Research Center (DRC) affiliate investigators with state-of-the-art vectors necessary to overexpress, knockdown, knockout, or alter expression of RNAs and proteins of interest in cultured cells, isolated tissues, and animals.

To accomplish this goal the VTMC provides the following to DRC affiliate investigators:

  • Production of lentiviral, adenoviral and retroviral vectors for use in animals, tissues, and cells;
  • Production of lentiviral constructs for generation of transgenic and knockdown mice;
  • Production of non-viral vectors for generation of transgenic, knockout, and knockin mice;
  • Specialized molecular biology methods not routinely performed in affiliate investigators’ laboratories;
  • Cost-effective production of genetically engineered mice through the University of Washington (UW) Transgenic Resources Program;
  • Consultation and training

For further information see https://depts.washington.edu/diabetes/vtmc or contact:

Karin Bornfeldt, PhD, Director, 206-543-1681, bornf@uw.edu

Bill Osborne, PhD, Associate Director, 206-685-3315, wosborne@u.washington.edu

For Core service information contact:

Virus Core – Kelly Kernan, 206-543-4735, kelk@uw.edu

Molecular Biology and Vector Production Core – Brian van Yserloo, 206-221-6508,brianvy@uw.edu