The Cell Analysis Facility
The Cell Anaysis Faciltiy is located in the Department of Immunology in Room H-581 HSC. The facility provides a broad range of instrumentation and expertise for addressing experimental issues related to single cells. The Facility makes available state-of-the-art instrumentation and techniques while developing new ways of answering questions about the nature and function of cells. Not only is instrumentation available to the University community, but it is intended that the Facility contribute to all aspects of the research process. This includes consultation in experimental design, technical assistance in the operation of the instruments, distribution of raw data files, archival data storage, offline data analysis workstations, interpretation of results, and the provision of facilities for the preparation and production of presentation graphics.
The facility hosts a server that provides over 1TB of on-line access to link investigators and their laboratories to their collected data from anywhere using FTP. Workstations for data analysis are available in the computer lab free of charge.
For instruction, introduction to flow cytometry courses are offered periodically, and individual instruction can be provided as needed. Please call us at 543-7006 for details.
For details of availability, time, and charges, please see the section on Frequently Asked Questions
Important Announcements
Upcoming Events:
Multicolor Flow Cytometry talk-May 16th: 12:15 – 1:30 pm South Campus Center Room 354
Optimizing Performance and Experimental Design
Elizabeth Trahan, Technical Applications Specialist BD Biosciences. For more information click here .
Seattle Advanced Cytometry Seminar-May 1st:The third of the monthly seminar series will be held here at the Department of Immunology. Dr. Jordan Jarjour the Director of Research and Development at Pregenen Inc. will be speaking. His talk entitled "Engineering enzymes using flow cytometry" will address a unique way of using flow to perform targeted gene repair in cells. Check out the flyer for more information.
This series is meant to highlight advancments in cytometry techniques and instrumentations. It is open to all in the Greater Seattle community. For more information or visit the website for all upcoming events. www.sacss.org
Users can now Subscribe to facility Announcements:Using the new online calendar, users can now subscribe to general announcements or subscribe to specific instrument updates that will be sent via email. This will provide timely information and alerts. Users are free to sign up for one or all lists that are of an interest. Instructions on how to subscribe are here.
New Scheduling Calendar:New online calendar will be going live in November. Please update your bookmarks to the new location. Note you will need to login with you UWNetID to schedule time. If you do not have a UWNetID, please see Michele Black to be added to the access list. See Scheduling for more detailed information.
Any material requiring BSL-2 containment or any viable cells that could be classified as BSL-2 (this is all human cell lines and primary human samples ) must be cleared through EH&S by submitting a Research Project Hazard Assessment (RPHA) form prior to being sorted at the facility.
When signing up for a sort please indicates the cells origin and you must specify the Biosafety level of your material. If it falls under BSL-2 you may not sort unless your lab is approved.
Approval will be given on a case by case basis through EH&S depending on the risk assessment as it applies specifically to cell sorting in the Cell Analysis Facility. Contact ehsbio@uw.edu to obtain the necessary forms and instructions to add the Cell Analysis Facility to your approved protocol.
Please refere to Sorting Requirements for additional information.
If you have any questions or any concerns please contact Michele Black at mblack2@u.washington.edu,
or 685-3014.
