The Cell Analysis Facility


The Cell Analysis Facility is currently located in the Department of Immunology at the UW Medicine South Lake Union Campus Room E-386.

The facility provides a broad range of instrumentation and expertise for addressing experimental issues related to single cells. The Facility makes available state-of-the-art instrumentation and techniques while developing new ways of answering questions about the nature and function of cells. Not only is instrumentation available to the University community, but it is intended that the Facility contribute to all aspects of the research process. This includes consultation in experimental design, technical assistance in the operation of the instruments, distribution of raw data files, archival data storage, offline data analysis workstations, interpretation of results, and the provision of facilities for the preparation and production of presentation graphics.

The facility hosts a server that provides over 10TB of on-line access to link investigators and their laboratories to their collected data from anywhere using FTP. Workstations for data analysis are available in the computer lab free of charge.

For instruction, introduction to flow cytometry courses are offered periodically, and individual instruction can be provided as needed. Please call us at 543-7006 for details.

For details of availability, time, and charges, please see the section on Frequently Asked Questions

Remember to acknowledge the valuable services provided by the Cell Analysis Facility in your research papers and publications: "This research was supported by the Cell Analysis Facility Flow Cytometry and Imaging Core in the Department of Immunology at the University of Washington."

Important Announcements

Upcoming Events:SACSS talk

TBD

 

The Seattle Advanced Cytomerty Series is meant to highlight advancments in cytometry techniques and instrumentations. It is open to all in the Greater Seattle community. For more information or visit the website for all upcoming events. sacss.org

Introduction to Fluoresence and Flow Cytometry - August 2014 please contact Michele for date and times

Rm E-301 12:00 - 1:00pm: This is offered as an informal talk by Michele Black on the basics of flow cytometry. Future talks will elaborate on specific topics related to flow cytometry. Open to all.

 

FlowJo logoFlowJo site license is now available for the University of Washington. Click here for more information.
 

 

Users can now Subscribe to facility Announcements: Using the new online calendar, users can now subscribe to general announcements or subscribe to specific instrument updates that will be sent via email. This will provide timely information and alerts. Users are free to sign up for one or all lists that are of an interest. Instructions on how to subscribe are here.

Scheduling Calendar: You will need to login with you UWNetID to schedule time. If you do not have a UWNetID or have not created an account with the facility, please see Michele Black to be added to the access list. See Scheduling for more detailed information.

Any material requiring BSL-2 containment or any viable cells that could be classified as BSL-2 (this is all human cell lines and primary human samples ) must be cleared through EH&S by submitting a Biological Use Authorization form (BUA) form prior to being sorted at the facility.

When signing up for a sort please indicates the cells origin and you must specify the Biosafety level of your material. If it falls under BSL-2 you may not sort unless your lab is approved.  

Approval will be given on a case by case basis through IBS commitee and EH&S depending on the risk assessment as it applies specifically to cell sorting in the Cell Analysis Facility. Contact ehsbio@uw.edu to obtain the necessary forms and instructions to add the Cell Analysis Facility to your approved protocol.

Please refere to Sorting Requirements for additional information.

 

If you have any questions or any concerns please contact Michele Black at mblack2@u.washington.edu
or  685-3014.

 

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