Frequently Asked Questions
- Whom to contact
- Where we are located
- Hours and Rates
- New User Information
- How to Access Data
To schedule time on an instrument, use the on-line calendar. For consultation on experimental design or interpretation of results, or for training to use any of the instruments in the facility, you can call Michele Black at 685-3014 or email her at firstname.lastname@example.org . Thane Mittelsteadt: email@example.com or Bryan Kennedy: firstname.lastname@example.org can be reached at the main lab phone # 543-7006.
We are located at the UW Medicine South Lake Union Campus in the 750 Rebublican St (E-building) on the 3rd floor. Please refer to this map for details of how to get to the facility. Please do not transport samples through the west hallway (office area). All samples must travel through the equipment corridor.
For all members of the SLU campus, your proxy key card will grant you access to the facility during normal business hours. All main campus users that use the facility on a regular basis can get a permanent card to the E building and to the flow facility. All afterhours users will need to obtain afterhours access by contacting Michele Black with your SLU keycard number.
Occasional users of the facility, both UW and external users will need to check in at the E building reception desk and obtain a temporary access card to be returned at the end of the day.
Regular Business Hours:
Monday - Fri day 9:00am - 5:00pm
The facility is intended to be self-supporting recharge center. The following rates are for internal UW accounts and external (non-UW affiliate) accounts. External accounts have an additional a 15.6% surcharge for UW overhead:
Time is billed to valid UW Budget numbers or PO for external users.
Internal Rate External Rate FACsAria Assisted Sorting: $97.00/hour $116.00/hour FACsAria After Hours Sorting: $65.00/hour* FACSCanto 2 laser: $53.00/hour $63.00/hour FACSCanto 3 laser: $55.00/hour $66.00/hour FACSCanto RUO: $62.00/hour $75.00/hour LSR II: $64.00/hour $77.00/hour ImageStreamX: $145.00/hour $174.00/hour NanoPro Run: $155.00/run $186.00/run NanoPro Cycle: $60.00/cycle $72.00/cycle FlowJo site License: $253.00/year
Computer use: no charge
* Must be trained by staff prior to being granted after hours access
Charges for time are rounded up to the nearest quarter hour. Rates are effective 07-01-2014.
Sorting may be scheduled between 9:00 A.M. to 5:00 P.M. (extended time available for trained after-hours users) Monday through Friday or by special arrangement with the operators. The facility also has multiple analyzers that can be used at any time. All users MUST be trained to operate these instruments and those who need to use the facility in off hours can be granted proxy card access. For information, please call the facility at 543-7006.
All new users need to complete the New User Form prior to gaining access to the facility and cytometers. The Staff will set up a new user account once the form is completed. The facility is accessible to all researchers at the University of Washington as well as users from other institutions. Please refer to the Rates and Hours for cost breakdown.
All users need to provide a current Budget # or PO# in order to set up an account. It is the users responsibility to enter that number in the Billing computer each time they use an instrument and to ensure that if the Budget # expires that they provide a replacement #.
Training on the cytometers is done by one on one instruction with the facility staff. It is most successful when the user has samples that they would like to analyze to customize the training to the individual. Users that are new to this facility but have previous cytometry experience still need to go through a brief orientation to become familiar with the Cell Analysis Facility operational practices. To set up training use the on-line calendar to book the instrument that you would like to use (see Scheduling for more information). If you are unsure of what instrument you need contact the facility or visit the Instrumentation page for information about each of the cytometers capabilities.
Training for sorting requires that you first have approval to sort. Please refer to Sorting Requirements prior to scheduling a sort.
We aim to train all user so that they are able to run the cytometer independently but are always available to help if needed.
After Hours Training
Users who wish to gain after hours access need to complete additional training. After hours access requires the user to have a Health Sciences Building Prox card. This is issued through the users Home Department, the facility cannot grant access without this card. Please plan ahead! Obtaining access can take several days and occasionally the building management system does not get the card activated on the first try. To verify that a card is active for the facility the user must attempt to open the main facility door after 5:30PM or weekends.
Steps to getting after hours access:
1. Contact facility staff and schedule an after hours training session. You will need to book this time on the instrument calendar for the instrument you are planning to use.
2. Bring your prox card! We cannot give you access without the prox card #.
3. After training, give your card number to Michele Black in person or through email.
4. Check that the card is active prior to needing access to the facility afterhours.
Gaining after hours sorting access requires demonstrating proficiency durring several sorts with staff supervision.
There are several ways to access your flow data.
The computer lab inside the facility has 5 iMAC's that are free to use to analyze data. Each workstation has the latest version of FlowJo. There is one PC in the lab with IDEAS imaging software.
On the Mac's, you can connect to the server through the "Go" menu and then “Connect to server". Select the immun-black.medicine.washington.edu/users share point and enter netid\UWNetID and password.
A "users" share will mount on the desktop. Open and find the folder with your userID. All of you current data from all instruments will be avaiable.
To access data from outside of the facility or from a PC follow these instructions to access from a Mac follow these instructions
Please contact the facility is you are having difficulties accesing your data.