Instrumentation
- Aria
- LSR II
- FACS CantoII
- FACS Canto
- FACScan
11* Color High speed 4-way Sorter (2 BSL-2 approved units)
$103.00/hour
$76.00/hour (after hours)
The Aria II combines the sensitivity of a digital bench-top analyzer with the functionality of a high precision sorter. Improvements in fludics and optics have allowed for higher speed sorting (25,000 events/sec at 70psi using a 70um nozzle) with less stress on the sorted cells and enhanced overall sensitivity.
Aria 1 uses fiber optic guided solid state 488nm 100mw, 637nm 150mw and 407nm 100mw diode lasers along with fiber guided emission collections. It has 3 detectors off of the violet laser.
Aria 2, has the added improvement of direct laser alignment to the flow cell eliminating the fiber optics while conserving more power from the source. It is configured very similarly to the Aria 1 but there are some minor differences that may require adjusting your settings in order for your samples to be properly compensated please refere to the optical configurations for the exact set up of each sorter. Please speak with Michele or Thane if you are having issues or need assistance in setting up.
Refer to Aria Sample Prep for information on what controls are needed and a guide to optimize samples for sorting. Both sorters are equipped with an aerosol management system (AMS) designed to decrease the risk of operating in a jet air sorter. Please review the Sorting Requirements prior to scheduling time on the sorters.
Aria 1 default optical configuration
Aria 2 default optical configuration
14 Color Analyzer
$71.00/hour
The LSR II is equipped with 5 lasers; 488nm blue laser, 561 nm yellow/green laser, 640nm red diode laser, 405nm violet laser, and 350nm UV laser. Capable of simultaneous detection of 14 fluorochromes and 16 parameter data acquisition. It is a digital instrument using FACSDiva software. The HTS plate reader accommodates 96-well plates for more automated high speed acquisition of samples.
8 Color Analyzer with HTS
$66.00/hour
The CantoII is equiped with 3 lasers; 488nm four colors detection and 633nm 2 color detection and 405nm 2 color detection. This unit is equipped with a High Throughput System (HTS) for collecting samples in a 96 well plate.
6 Color Analyzer (2 units)
$62.00/hour
The Canto's are equiped with 2 lasers; 488nm four colors detection and 633nm 2 color detection. They are digital instruments using FACSDiva software.
3 Color Analyzer
$34.00/hour
The FACScan is capable of 3 color detection off a 488nm laser. It is an economical choice for DNA cell cycle or screening for eGFP and other simple assays.
