The cell sorting room H-581C is approved for work at Biosafety Level 1 and 2 (BSL-1 and BSL-2). Room H-581C is maintained under negative pressure relative to the larger lab space.  All cells exposed to or infected with bacterial or viral agents must be approved by EH&S on a case by case basis.

Approval to Sort

Please refer to the Sorting Requirements page for complete details on obtaining approval. Once a lab has recieved approval from EH&S user from that lab may sign up to sort in the facility.

Sign-up 

Each user is responsible for signing up using the on-line calendar

Please contact the facility if you are having difficulties logging on to the new calendar.
There are new features and requirements when signing up.

Your phone # is a mandatory field.
Enter your email address in the popup window along with anyother information you would like to convey.

The Aria’s require that you select the nozzle size and other options for sorting. They still require approval but will be visible once you enter a request.

The Canto 3 and LSRII have a HTS option, the default is NO:  if you are using the HTS select YES: and remember to add 30 minutes total (15 minutes setup and 15 minutes clean) to your scheduled time.

You can now send email reminders to yourself or send staff notifications about your scheduled time through the entry form. You can also subcribe to facility updates and instrument specific notifications. Use the Email Subscription function on the calendar.

When you are done using the calendar make sure to completely close your browser to logout if you are using a shared computer.

New Instrument Calendar

Sign up for sorting time in 15 minute increments, with an extra 15 minutes included at the end of the sort for decontamination and cleaning. 

1. Sign-up information MUST include: user name, sample description, contact phone number and email.
2. All BSL-2 sorts must also include a distinct BSL-2 notation as well as the PI name that the BUA approval is under. Please refere to the Biosafety Table for examples of samples that require BSL-2 containment and the safety requirements.
3. Each entry is approved after the Facility Staff has looked at the request and made sure that there will be appropriate staff and all the required information is provided.

Cell Analysis Facility staff is available for presort consultation.

You MUST contact CAF staff at 543-7006 prior to scheduling a same day sort to ensure you can be accommodated.

All sorts scheduled are forwarded to the staff via e-mail for approval prior to appearing on the calendar. To expedite your request to reserve time provide detailed information regarding your sort including;

Once you have submitted a request that time slot is secured and will appear on the calendar after the staff verifies that you can be accommodated.

Please note the following:

If you cancel less than 24 hours in advance, you will be charged for 1 hour of set-up time.

No shows will be charged for the full amount of time they have reserved.

Type of cell/ procedure	Exempt BSL-1	BSL-1	BSL-2
Examples of Cells	Wild type cells from murine or other non-human / non-primate species that have NOT been exposed to any microbial agent (e.g., viral, bacterial, fungal, protozoan, parasitic) and have not been genetically modified or Cells determined by EH&S and the IBC to be recombinant NIH-exempt BSL-1.	Cells from murine or other non-human / non-primate species that have NOT been exposed to any microbial agent but have been genetically modified using non-viral methods (e.g. cells from transgenic animals or cells treated with nucleic acids). or Cells determined by EH&S and the IBC to be approved as non-recombinant BSL-1 or recombinant BSL-1.	Cells from human or non-human primates or Cells that have been genetically modified using viral methods or Cells exposed to microbial  agents (e.g., viral, bacterial, fungal, protozoan, parasitic) AND Have been approved by EH&S and the IBC for BSL-2 containment and sorting
BUA update to sort in the Immunology CAF	Not required.	Required.	Required.
Bloodborne Pathogen (BBP) Training	Not required.	Not required.	Initial and annual renewal required for work with human cells and other cells exposed to bloodborne pathogens.
Sort Sign-up	Required. Include name, phone number, sample description.	Required. Include name, phone number, sample description and a clear BSL-1 notation.	Required. Include name, phone number, sample description and a clear BSL-2 notation.
Sample Transport	No requirement	Leak-proof secondary container.	Leak-proof secondary container.
Room Restriction	None.	None.	YES – door to H-581C must be closed during sort and the BSL-2 Biohazard sign posted on the outside.
Personal Protective Equipment (PPE)	Closed toe shoes.  When manipulating samples (i.e. loading/unloading samples), gloves, lab coat, and face protection are recommended.	Closed toe shoes. When manipulating samples (i.e. loading/unloading samples), gloves, lab coat, and face protection are strongly recommended. Spills: Lab coat, nitrile gloves, goggles/faceshield required.	Closed toe shoes, lab coat and gloves required at all times. When manipulating samples (i.e. loading/unloading samples), face protection is also required. Spills: Lab coat, nitrile gloves, goggles/faceshield required.
Waste	Contaminated materials must be decontaminated prior to disposal.	Gloves and other waste must be disposed of as biohazardous. See CAF SOP.	Gloves and other waste must be disposed of as biohazardous. See CAF SOP.

 

Calendar Entry Instructions

The calendar will default to the current day. Choose the day you would like to schedule and appointment on the Menu bar. Once on the the correct day click on the "+" next to the instrument you would like to reserve (notice the arrow cursor changes to a finger), a pop-up window will appear. Fill out the form as indicated. Click the "Submit" button when you're done to post your information to the calendar.

Individual monthly calendars are available by clicking on the “Select” button on the Daily Schedule a popup window will open to select the monthly calendar needed. To schedule from the monthly calendars, click on the underlined number for the day , a list of already scheduled appointments will appear in a pop-up window. Click on "Create" a pop-up window like the one below will appear. Fill out the form as indicated. Click the "Submit" button when you're done to post your information to the calendar. * Reminder that time scheduled on the Aria's will not appear until the staff varifies we have all the needed information

 

Daily Instrument Schedule