The Cell Analysis Facility


The Cell Analysis Facility is located in the Department of Immunology at the UW Medicine South Lake Union Campus Room E-386.

The facility provides a broad range of instrumentation and expertise for addressing experimental issues related to single cells. The Facility makes available state-of-the-art instrumentation and techniques while developing new ways of answering questions about the nature and function of cells. Not only is instrumentation available to the University community, but it is intended that the Facility contribute to all aspects of the research process. This includes consultation in experimental design, technical assistance in the operation of the instruments, distribution of raw data files, archival data storage, offline data analysis workstations, interpretation of results, and the provision of facilities for the preparation and production of presentation graphics.

The facility hosts a server that provides over 10TB of on-line access to link investigators and their laboratories to their collected data from anywhere. Workstations for data analysis are available in the computer lab free of charge.

For instruction, introduction to flow cytometry courses are offered periodically, and individual instruction can be provided as needed. Please call us at 543-7006 for details.

For details of availability, time, and charges, please see the section on Frequently Asked Questions

Remember to acknowledge the valuable services provided by the Cell Analysis Facility in your research papers and publications: "This research was supported by the Cell Analysis Facility Flow Cytometry and Imaging Core in the Department of Immunology at the University of Washington."

CAF COVID-19 Policies

Data Analysis Computer Lab: The workstation in the Cell Analysis Facility are closer together than the recommended 6-foot social distancing outlined by the CDC. We are limiting the number of people that are in that space at any given time. Please give others sufficient space and if there are more than One other person using the computers please wait until they have left before entering the computer room and choose a workstation furthest away from others. Working on data on your personal computer outside of this shared space is preferred.
FlowJo has extended a free 30 day license for user so that they can work remotely instead of using computer that are in a shared lab. If you would like to get a temporary license that you can use from your personal computer, follow the link below. Please be patient as it may take a couple of days for FlowJo to grant access to everyone that is requesting it. Contact me by email for more information.
Instrumentation Use: Cell sorting and analysis users that are self-sufficient may sign up for time on the cytometers. If you require assistance for a critical experiment contact the facility prior to scheduling. All others please Do Not schedule, the facility staff is practicing social distancing which limits training or assisting users that need hands on help to run at this time. 
Self-user scheduling: Instruments that have been scheduling in advance will be setup by the facility staff. If you sign up on the same day and no one else has already scheduled time on that instrument the instrument will not be turned on by the staff. You may email flowlab@uw.edu or call the lab (206) 543-7006 to request it be turned on and QC’d.  If we are in the lab still setting up, we can turn on the cytometer for you.

Only one person at an instrument at a time. Do not come in groups to run samples. Designate a single person to enter the facility. Please review the facility map Here

Due to the close proximity of the CantoRUO and Canto2 do not sign up for time that coinsides with an other persons timeblock on the other instrument.


All users who run should be familiar with how to properly shutdown the instruments. Please follow the weekend self user guides for scheduling and being responsible for startup and shutdown if you are scheduling the same day. Any user regardless if same day or in advance scheduling should perform the instrument shutdown if they are the last user for the day.
Cancellations: If you have scheduled in advance and need to cancel day of, make sure to email flowlab@uw.edu 1st thing in the morning so that we can remove you from the schedule.
If you have a cough, sore throat, chills or fever STAY HOME.
Please follow these procedures when entering the Facility.


Before entering the lab, please have mask or face covering on.
Cytometer Usage:

  1. Don new gloves before using a cytometer (gloves located by the doors)
  1. Decontaminate the workstation.
    1. Spray the keyboard, mouse and surrounding surfaces with 70% EtOH let sit 5 minutes. Wipe dry with a paper towel and discard in the trash. 
  2. Login and run your experiment.
  3. When finished, follow the posted instrument cleaning procedure. In addition, decontaminate the workstation again.
    1. Lightly spray the keyboard, mouse and surrounding surfaces with Envirocide (white bottle with green and Purple writing) some people are more sensitive to this product and you should avoid getting it on your skin. 
    2. Let sit for 5 minutes
    3. Spray the same surfaces with 70% EtOH and wipe dry with paper towels, discard in the trash.
  4. Remove gloves and discard before leaving the instrumentation rooms.

Data analysis workstation:

  1. Sanitize hands 1st before sitting down at a workstation in the Computer lab.
  1. Decontaminate the workstation.
    1. Spray the keyboard, mouse and surrounding surfaces with 70% EtOH let sit 5 minutes. Wipe dry with a paper towel and discard in the trash. 
  2. When finished, decontaminate the workstation again.
    1. Lightly spray the keyboard, mouse and surrounding surfaces with Envirocide (white bottle with green and Purple writing) some people are more sensitive to this product and you should avoid getting it on your skin. 
    2. Let sit for 5 minutes
    3. Spray the same surfaces with 70% EtOH and wipe dry with paper towels, discard in the trash.

New Rates effective September 01, 2019

Rates are seeing an increase this year due to a combination of factors; lower that anticipated usage and higher operational costs. The Cell Analsysis Faciltiy is a recharge center and by federal law cannot make a profit and must cover all of its operational expenses by the rates charged to the users. In order to lower some expenses, the Canto 1 (2 laser system) has been retired. It was determined that there is ample room on the CantoRUO and Canto 2 (3 laser system) to accomodate the needs of current uses of the Canto 1. If you have questions, please contact Michele.

Our Cytek Aurora upgrade is complete!

The Aurora now has a UV laser. The 5 laser system is capable of running panels >30 colors. Check out the full list of dyes compatible with the system on the Instrumentation page. As with any cytometer, careful panel design and propper controls are still needed. Please contact the Flowlab for more information and help designing panels for any of the cytometers available in the lab.

 

 

Other News

Users can now Subscribe to facility Announcements: Using the new online calendar, users can now subscribe to general announcements or subscribe to specific instrument updates that will be sent via email. This will provide timely information and alerts. Users are free to sign up for one or all lists that are of an interest. Instructions on how to subscribe are here.

Scheduling Calendar: You will need to login with you UWNetID to schedule time. If you do not have a UWNetID or have not created an account with the facility, please see Michele Black to be added to the access list. See Scheduling for more detailed information.

Any material requiring BSL-2 containment or any viable cells that could be classified as BSL-2 (this is all human cell lines and primary human samples ) must be cleared through EH&S by submitting a Biological Use Authorization form (BUA) form prior to being sorted at the facility.

When signing up for a sort please indicates the cells origin and you must specify the Biosafety level of your material. If it falls under BSL-2 you may not sort unless your lab is approved.  

Approval will be given on a case by case basis through IBS commitee and EH&S depending on the risk assessment as it applies specifically to cell sorting in the Cell Analysis Facility. Contact ehsbio@uw.edu to obtain the necessary forms and instructions to add the Cell Analysis Facility to your approved protocol.

Please refere to Sorting Requirements for additional information.

 

If you have any questions or any concerns please contact Michele Black at mblack2@uw.edu
or  685-3014.

 

Top of Page