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Basic Molecular Biology Techniques |
Contents |
General Overview |
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| Primer on Molecular Genetics -- html format |
The Department of Energy's 44-page primer on basic molecular biology. Why DOE? Because they were among the first to get involved with the human genome project. The primer reflects that goal -- it is strongly slanted towards explaining the techniques behind the human genome project, but it does cover many standard techniques (in simple language) along the way. Note that this article was written in 1992, so some of the information (e.g., the amount of DNA sequence available) is out of date. |
| Primer on Molecular Genetics -- pdf | Same as above, as a downloadable PDF file (732 kilobytes). Printed versions of this file will be placed on reserve in Odegaard Library. |
| DNA Technology | A very comprehensive set of links to various web sites describing molecular biology techniques |
Restriction endonucleases |
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| Restriction enzymes | A very brief look at restriction enzymes and their discovery |
Gel electrophoresis, Southern blotting and hybridization |
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| Gel electrophoresis animation | Follow the link back from this animation page for more info on electrophoresis... |
| Gel Electrophoresis Movie | QuickTime animation depicting gel electrophoresis (1220 KB) |
| Gel electrophoresis, Southern blot, more... | Overview with static images |
| Southern Blotting: Gel Transfer | Diagram illustrating Southern transfer |
| Southern Blotting animation | Animation of the whole process -- gel electrophoresis, transfer of the DNA to the filter, hybridization. Requires Flash plug-in. |
Polymerase Chain Reaction (PCR) |
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| PCR | Diagram illustrating PCR |
| Bare bones PCR animation | Only one round of amplification shown; focus is on primer choice (with a sample DNA sequence shown). |
| PCR animation | Requires Flash plug-in. |
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