Genetics 551 FALL 2000
Segment 1
Celeste A. Berg berg@genetics.washington.edu
543-1677 J-229B
Segment 2
Colin Manoil manoil@u.washington.edu
543-7800 J-127
Syllabus
Segment 1 Berg
Part A Introduction to Genetic Analysis
M Sep 25 Introduction to course. Why do genetic analysis? Introduction to yeast system, mating pathway, nomenclature.
Assigned: Sullivan.
Optional:
Yeast DataBase: http://www.mips.biochem.mpg.de/
Book Chapter/Gene: Yeast Experiments: http://www.phys.ksu.edu/gene/a1.html
OTHER REFERENCES:
Guthrie, C. and Fink, G.R., Editors. Guide to Yeast Genetics and Molecular Biology. Methods in Enzymology, Volume 194, Academic Press, New York, 1991.
Broach, J.R., Pringle, J.R. and Jones, E.W., Editors. The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1997.
W Sep 27 Isolation of mutants; phenotypic characterization.
Assigned: Hartwell. 1980. J. Cell Biol. 85: 811-822.
F Sep 29 Double mutant analysis.
Assigned: Hereford and Hartwell. 1974. J. Mol. Biol. 84: 445-461.
M Oct 02 More double mutant analysis; redundancy.
Assigned: Whiteway et al. 1989. Cell 56: 467-477.
W Oct 04 Suppressors, dominants.
Assigned: Stevenson et al. 1992. Genes Dev. 6: 1293-1304.
F Oct 06 Pleiotropy.
Assigned: Roberts and Fink. 1994. Genes Dev. 8: 2974-2985.
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Part B Genetic Analysis in Multi-cellular Organisms
M Oct 09 Introduction to genetics in multi-cellular organisms; Specifics of fly genetics and nomenclature; Introduction to Drosophila eye development.
Assigned: Greenspan. 1997. Fly Pushing Chapter 1: 3-17.
W Oct 11 Isolation of mutants; mosaic analysis.
Assigned: Reinke and Zipursky. 1988. Cell 55: 321-330.
F Oct 13 Defining the pathway: enhancer screens.
Assigned: Simon et al. 1991. Cell 67: 701-716.
M Oct 16 Gain of function mutations: the complications of pleiotropy.
Assigned: Brunner et al. 1994. Cell 76: 875-888.
W Oct 18 Parallel pathways: suppressor screens.
Assigned: Raabe et al. 1996. Cell 85: 911-920.
F Oct 20 Redundancy of function; redundancy of pathway.
Assigned: Kockel et al. 1997. Genes Dev. 11: 1748-1758.
M Oct 23 Recap.
Assigned: Pathway analysis in Hallucigenia creativa
October 25 Two-part take home Exam; individual effort only.
October 27 Work on Exam.
M October 30 Exam Due, BEGINNING of class.