MBT510/GENET551 Fall 2001

Syllabus Segment 3

Celeste A. Berg berg@genetics.washington.edu 543-1677 J-229B

Introduction to Genetic Analysis

Tues Nov 20 Introduction to segment 3. Why do genetic analysis? What is genetic analysis? Introduction to yeast system, mating pathway, nomenclature.

Assigned: Sullivan.

Optional: Yeast DataBase: http://www.mips.biochem.mpg.de/

Book Chapter/Gene: Yeast Experiments: http://www.phys.ksu.edu/gene/a1.html

OTHER REFERENCES:

Guthrie, C. and Fink, G.R., Editors. Guide to Yeast Genetics and Molecular Biology. Methods in Enzymology, Volume 194, Academic Press, New York, 1991.

Broach, J.R., Pringle, J.R. and Jones, E.W., Editors. The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1997.

Tues Nov 27
questions for thought
Isolation of mutants; phenotypic characterization.

Assigned: Hartwell. 1980. J. Cell Biol. 85: 811-822.

Thurs Nov 29
questions for thought
Double mutant analysis.

Assigned: Hereford and Hartwell. 1974. J. Mol. Biol. 84: 445-461.

Tues Dec 4
questions for thought
More double mutant analysis; redundancy; cloning.

Assigned: Whiteway et al. 1989. Cell 56: 467-477.

Thurs Dec 6
questions for thought
Suppressors, dominants, parallel pathways.

Assigned: Stevenson et al. 1992. Genes Dev. 6: 1293-1304.

Tues Dec 11
paper
questions for thought
Overexpression and knock-out strategies.

Assigned: Stevenson, Kennedy and Harlow. 2001. PNAS 98: 3946-3951.

Mon Dec 17
paper
Spellman paper
Exam Due, 5 pm.

Assigned: Cho, Campbell, Winzeler, et al. 1998. Molec. Cell 2: 65-73.

* Master copy of papers will be on reserve in the copy room, J205. Use budget number 0436 to xerox your copy. PLEASE DO NOT TAKE OR WRITE ON THE MASTER COPIES.

* “Questions for Thought” will be handed out in class and posted on the web: http://depts.washington.edu/genetics/courses/courses.html